Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c23780 and PGA1_c23790 are separated by 37 nucleotides PGA1_c23790 and PGA1_c23800 are separated by 139 nucleotides PGA1_c23800 and PGA1_c23810 are separated by 168 nucleotides PGA1_c23810 and PGA1_c23820 overlap by 1 nucleotides
PGA1_c23780: PGA1_c23780 - putative integral membrane protein, at 2,481,094 to 2,482,056
_c23780
PGA1_c23790: PGA1_c23790 - thiamine pyrophosphokinase-like protein, at 2,482,094 to 2,482,759
_c23790
PGA1_c23800: PGA1_c23800 - hypothetical protein, at 2,482,899 to 2,485,169
_c23800
PGA1_c23810: PGA1_c23810 - Protein of unknown function (DUF2842)., at 2,485,338 to 2,485,583
_c23810
PGA1_c23820: PGA1_c23820 - adenylosuccinate synthetase PurA, at 2,485,583 to 2,486,896
_c23820
Position (kb)
2482
2483
2484
2485
2486 Strain fitness (log2 ratio)
-1
0
1
2 at 2481.902 kb on - strand, within PGA1_c23780 at 2481.981 kb on + strand at 2482.022 kb on - strand at 2482.100 kb on - strand at 2482.743 kb on + strand at 2482.840 kb on + strand at 2482.848 kb on - strand at 2482.924 kb on - strand at 2482.936 kb on - strand at 2482.951 kb on + strand at 2483.087 kb on + strand at 2483.106 kb on + strand at 2483.120 kb on - strand at 2483.125 kb on - strand at 2483.131 kb on + strand, within PGA1_c23800 at 2483.176 kb on + strand, within PGA1_c23800 at 2483.435 kb on + strand, within PGA1_c23800 at 2483.435 kb on + strand, within PGA1_c23800 at 2483.435 kb on + strand, within PGA1_c23800 at 2483.435 kb on + strand, within PGA1_c23800 at 2483.450 kb on + strand, within PGA1_c23800 at 2483.458 kb on - strand, within PGA1_c23800 at 2483.480 kb on + strand, within PGA1_c23800 at 2483.503 kb on - strand, within PGA1_c23800 at 2483.580 kb on - strand, within PGA1_c23800 at 2483.702 kb on - strand, within PGA1_c23800 at 2483.809 kb on + strand, within PGA1_c23800 at 2483.819 kb on + strand, within PGA1_c23800 at 2483.914 kb on - strand, within PGA1_c23800 at 2483.949 kb on + strand, within PGA1_c23800 at 2483.965 kb on + strand, within PGA1_c23800 at 2484.046 kb on - strand, within PGA1_c23800 at 2484.331 kb on - strand, within PGA1_c23800 at 2484.344 kb on - strand, within PGA1_c23800 at 2484.549 kb on + strand, within PGA1_c23800 at 2484.635 kb on + strand, within PGA1_c23800 at 2484.643 kb on - strand, within PGA1_c23800 at 2484.919 kb on + strand, within PGA1_c23800 at 2484.999 kb on + strand at 2485.007 kb on - strand at 2485.021 kb on + strand at 2485.030 kb on + strand at 2485.071 kb on - strand at 2485.123 kb on + strand at 2485.170 kb on + strand at 2485.170 kb on + strand at 2485.170 kb on + strand at 2485.178 kb on - strand at 2485.178 kb on - strand at 2485.178 kb on - strand at 2485.178 kb on - strand at 2485.178 kb on - strand at 2485.237 kb on - strand at 2485.238 kb on + strand at 2485.238 kb on + strand at 2485.246 kb on - strand at 2485.305 kb on - strand at 2485.366 kb on + strand, within PGA1_c23810 at 2485.367 kb on + strand, within PGA1_c23810 at 2485.411 kb on + strand, within PGA1_c23810 at 2485.414 kb on - strand, within PGA1_c23810 at 2485.474 kb on - strand, within PGA1_c23810 at 2485.526 kb on + strand, within PGA1_c23810 at 2485.545 kb on - strand, within PGA1_c23810
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 2,481,902 - PGA1_c23780 0.84 +0.1 2,481,981 + -0.0 2,482,022 - +0.3 2,482,100 - -0.9 2,482,743 + +0.2 2,482,840 + +0.5 2,482,848 - +0.5 2,482,924 - +1.7 2,482,936 - +0.8 2,482,951 + +0.4 2,483,087 + +0.4 2,483,106 + +1.5 2,483,120 - -0.1 2,483,125 - +0.1 2,483,131 + PGA1_c23800 0.10 +1.2 2,483,176 + PGA1_c23800 0.12 +0.8 2,483,435 + PGA1_c23800 0.24 +2.7 2,483,435 + PGA1_c23800 0.24 +2.2 2,483,435 + PGA1_c23800 0.24 +0.5 2,483,435 + PGA1_c23800 0.24 +1.8 2,483,450 + PGA1_c23800 0.24 +1.0 2,483,458 - PGA1_c23800 0.25 +0.7 2,483,480 + PGA1_c23800 0.26 -0.1 2,483,503 - PGA1_c23800 0.27 +1.7 2,483,580 - PGA1_c23800 0.30 +0.3 2,483,702 - PGA1_c23800 0.35 +0.5 2,483,809 + PGA1_c23800 0.40 +0.3 2,483,819 + PGA1_c23800 0.41 +0.4 2,483,914 - PGA1_c23800 0.45 +2.0 2,483,949 + PGA1_c23800 0.46 +0.0 2,483,965 + PGA1_c23800 0.47 -0.1 2,484,046 - PGA1_c23800 0.51 +0.5 2,484,331 - PGA1_c23800 0.63 +0.5 2,484,344 - PGA1_c23800 0.64 +0.4 2,484,549 + PGA1_c23800 0.73 +0.2 2,484,635 + PGA1_c23800 0.76 +0.0 2,484,643 - PGA1_c23800 0.77 +0.8 2,484,919 + PGA1_c23800 0.89 +0.4 2,484,999 + +0.2 2,485,007 - +0.5 2,485,021 + -0.3 2,485,030 + -0.6 2,485,071 - +0.8 2,485,123 + +0.1 2,485,170 + +0.8 2,485,170 + -0.2 2,485,170 + -0.2 2,485,178 - -1.4 2,485,178 - +0.4 2,485,178 - +0.7 2,485,178 - -1.2 2,485,178 - -1.1 2,485,237 - +0.8 2,485,238 + +0.8 2,485,238 + -1.0 2,485,246 - +0.4 2,485,305 - +1.1 2,485,366 + PGA1_c23810 0.11 -0.4 2,485,367 + PGA1_c23810 0.12 -0.2 2,485,411 + PGA1_c23810 0.30 -0.1 2,485,414 - PGA1_c23810 0.31 -0.5 2,485,474 - PGA1_c23810 0.55 +0.1 2,485,526 + PGA1_c23810 0.76 -0.6 2,485,545 - PGA1_c23810 0.84 -0.3
Or see this region's nucleotide sequence