Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c14260 and PGA1_c14270 are separated by 119 nucleotides PGA1_c14270 and PGA1_c14280 are separated by 3 nucleotides PGA1_c14280 and PGA1_c14290 are separated by 5 nucleotides PGA1_c14290 and PGA1_c14300 are separated by 124 nucleotides
PGA1_c14260: PGA1_c14260 - peptidase M50, at 1,480,403 to 1,481,752
_c14260
PGA1_c14270: PGA1_c14270 - hypothetical protein, at 1,481,872 to 1,482,195
_c14270
PGA1_c14280: PGA1_c14280 - outer membrane protein Omp, at 1,482,199 to 1,484,595
_c14280
PGA1_c14290: PGA1_c14290 - putative outer membrane protein, at 1,484,601 to 1,485,251
_c14290
PGA1_c14300: PGA1_c14300 - (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase FabZ, at 1,485,376 to 1,485,840
_c14300
Position (kb)
1482
1483
1484
1485 Strain fitness (log2 ratio)
-2
-1
0
1 at 1481.237 kb on + strand, within PGA1_c14260 at 1481.401 kb on + strand, within PGA1_c14260 at 1481.401 kb on + strand, within PGA1_c14260 at 1481.401 kb on + strand, within PGA1_c14260 at 1481.401 kb on + strand, within PGA1_c14260 at 1481.646 kb on + strand at 1481.771 kb on + strand at 1481.779 kb on - strand at 1481.779 kb on - strand at 1481.786 kb on + strand at 1481.786 kb on + strand at 1481.786 kb on + strand at 1481.786 kb on + strand at 1481.786 kb on + strand at 1481.794 kb on - strand at 1481.794 kb on - strand at 1481.874 kb on + strand at 1481.938 kb on + strand, within PGA1_c14270 at 1481.945 kb on + strand, within PGA1_c14270 at 1481.947 kb on + strand, within PGA1_c14270 at 1481.948 kb on - strand, within PGA1_c14270 at 1482.018 kb on - strand, within PGA1_c14270 at 1485.230 kb on + strand at 1485.238 kb on - strand at 1485.285 kb on + strand at 1485.293 kb on - strand at 1485.295 kb on + strand at 1485.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 1,481,237 + PGA1_c14260 0.62 -2.1 1,481,401 + PGA1_c14260 0.74 -1.8 1,481,401 + PGA1_c14260 0.74 -1.8 1,481,401 + PGA1_c14260 0.74 +1.6 1,481,401 + PGA1_c14260 0.74 -1.6 1,481,646 + -1.6 1,481,771 + +0.5 1,481,779 - +0.4 1,481,779 - -0.0 1,481,786 + +0.4 1,481,786 + -0.1 1,481,786 + -0.1 1,481,786 + +0.8 1,481,786 + +0.4 1,481,794 - -0.6 1,481,794 - -0.6 1,481,874 + +0.6 1,481,938 + PGA1_c14270 0.20 -2.0 1,481,945 + PGA1_c14270 0.23 -0.1 1,481,947 + PGA1_c14270 0.23 -0.5 1,481,948 - PGA1_c14270 0.23 -1.4 1,482,018 - PGA1_c14270 0.45 -0.4 1,485,230 + -0.2 1,485,238 - -0.6 1,485,285 + +0.1 1,485,293 - +0.4 1,485,295 + -0.1 1,485,313 - +0.4
Or see this region's nucleotide sequence