Experiment: Ammonium chloride (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11450 and PGA1_c11460 are separated by 498 nucleotides PGA1_c11460 and PGA1_c11470 are separated by 37 nucleotides PGA1_c11470 and PGA1_c11480 overlap by 4 nucleotides PGA1_c11480 and PGA1_c11490 overlap by 4 nucleotides
PGA1_c11450: PGA1_c11450 - hypothetical protein, at 1,185,339 to 1,185,614
_c11450
PGA1_c11460: PGA1_c11460 - hypothetical protein, at 1,186,113 to 1,186,331
_c11460
PGA1_c11470: PGA1_c11470 - acetyltransferase domain-containing protein, at 1,186,369 to 1,186,818
_c11470
PGA1_c11480: PGA1_c11480 - Uncharacterized conserved protein, at 1,186,815 to 1,187,342
_c11480
PGA1_c11490: PGA1_c11490 - pyrimidine-specific ribonucleoside hydrolase RihA, at 1,187,339 to 1,188,280
_c11490
Position (kb)
1186
1187 Strain fitness (log2 ratio)
-2
-1
0
1 at 1185.413 kb on - strand, within PGA1_c11450 at 1185.645 kb on + strand at 1185.650 kb on - strand at 1185.664 kb on + strand at 1185.668 kb on - strand at 1185.685 kb on + strand at 1185.704 kb on + strand at 1185.735 kb on - strand at 1185.735 kb on - strand at 1185.818 kb on - strand at 1185.917 kb on + strand at 1185.920 kb on + strand at 1185.930 kb on + strand at 1185.938 kb on - strand at 1185.964 kb on + strand at 1186.008 kb on - strand at 1186.031 kb on + strand at 1186.031 kb on + strand at 1186.031 kb on + strand at 1186.089 kb on + strand at 1186.111 kb on + strand at 1186.111 kb on + strand at 1186.152 kb on - strand, within PGA1_c11460 at 1186.169 kb on - strand, within PGA1_c11460 at 1186.169 kb on - strand, within PGA1_c11460 at 1186.173 kb on + strand, within PGA1_c11460 at 1186.180 kb on + strand, within PGA1_c11460 at 1186.197 kb on - strand, within PGA1_c11460 at 1186.305 kb on - strand, within PGA1_c11460 at 1186.450 kb on - strand, within PGA1_c11470 at 1186.507 kb on - strand, within PGA1_c11470 at 1186.512 kb on - strand, within PGA1_c11470 at 1186.617 kb on - strand, within PGA1_c11470 at 1186.719 kb on - strand, within PGA1_c11470 at 1186.772 kb on + strand, within PGA1_c11470 at 1186.782 kb on + strand at 1186.785 kb on - strand at 1186.785 kb on - strand at 1186.785 kb on - strand at 1186.785 kb on - strand at 1186.897 kb on - strand, within PGA1_c11480 at 1186.897 kb on - strand, within PGA1_c11480 at 1186.899 kb on - strand, within PGA1_c11480 at 1186.961 kb on + strand, within PGA1_c11480 at 1186.999 kb on - strand, within PGA1_c11480 at 1187.032 kb on + strand, within PGA1_c11480 at 1187.077 kb on + strand, within PGA1_c11480 at 1187.197 kb on - strand, within PGA1_c11480 at 1187.278 kb on - strand, within PGA1_c11480 at 1187.301 kb on - strand at 1187.303 kb on + strand at 1187.311 kb on - strand at 1187.311 kb on - strand at 1187.313 kb on + strand at 1187.337 kb on + strand at 1187.337 kb on + strand at 1187.397 kb on + strand at 1187.405 kb on - strand at 1187.405 kb on - strand at 1187.515 kb on + strand, within PGA1_c11490 at 1187.515 kb on + strand, within PGA1_c11490 at 1187.515 kb on + strand, within PGA1_c11490 at 1187.518 kb on - strand, within PGA1_c11490 at 1187.523 kb on - strand, within PGA1_c11490 at 1187.542 kb on + strand, within PGA1_c11490 at 1187.595 kb on - strand, within PGA1_c11490 at 1187.666 kb on - strand, within PGA1_c11490 at 1187.707 kb on + strand, within PGA1_c11490 at 1187.725 kb on + strand, within PGA1_c11490 at 1187.725 kb on + strand, within PGA1_c11490 at 1187.735 kb on - strand, within PGA1_c11490 at 1187.740 kb on - strand, within PGA1_c11490 at 1187.740 kb on - strand, within PGA1_c11490
Per-strain Table
Position Strand Gene LocusTag Fraction Ammonium chloride (N) remove 1,185,413 - PGA1_c11450 0.27 +0.0 1,185,645 + +1.6 1,185,650 - -0.5 1,185,664 + +0.1 1,185,668 - +0.1 1,185,685 + +0.2 1,185,704 + -2.0 1,185,735 - +0.1 1,185,735 - +0.4 1,185,818 - +0.7 1,185,917 + -0.2 1,185,920 + -1.2 1,185,930 + +0.7 1,185,938 - -0.7 1,185,964 + -0.7 1,186,008 - -0.6 1,186,031 + -0.2 1,186,031 + +0.0 1,186,031 + -0.4 1,186,089 + -0.1 1,186,111 + +0.1 1,186,111 + +0.2 1,186,152 - PGA1_c11460 0.18 -1.0 1,186,169 - PGA1_c11460 0.26 +0.6 1,186,169 - PGA1_c11460 0.26 +0.2 1,186,173 + PGA1_c11460 0.27 -0.1 1,186,180 + PGA1_c11460 0.31 -1.0 1,186,197 - PGA1_c11460 0.38 +1.6 1,186,305 - PGA1_c11460 0.88 +0.7 1,186,450 - PGA1_c11470 0.18 +0.7 1,186,507 - PGA1_c11470 0.31 -0.4 1,186,512 - PGA1_c11470 0.32 -0.2 1,186,617 - PGA1_c11470 0.55 +1.1 1,186,719 - PGA1_c11470 0.78 +0.5 1,186,772 + PGA1_c11470 0.90 +0.5 1,186,782 + +0.6 1,186,785 - +0.2 1,186,785 - +1.0 1,186,785 - +0.3 1,186,785 - -0.1 1,186,897 - PGA1_c11480 0.16 -0.0 1,186,897 - PGA1_c11480 0.16 -0.7 1,186,899 - PGA1_c11480 0.16 -0.3 1,186,961 + PGA1_c11480 0.28 -0.3 1,186,999 - PGA1_c11480 0.35 +0.9 1,187,032 + PGA1_c11480 0.41 +0.4 1,187,077 + PGA1_c11480 0.50 -1.3 1,187,197 - PGA1_c11480 0.72 +1.6 1,187,278 - PGA1_c11480 0.88 +0.1 1,187,301 - +0.6 1,187,303 + +0.1 1,187,311 - +0.8 1,187,311 - -0.7 1,187,313 + -0.0 1,187,337 + +0.6 1,187,337 + +0.3 1,187,397 + +0.7 1,187,405 - +0.5 1,187,405 - +0.0 1,187,515 + PGA1_c11490 0.19 +0.1 1,187,515 + PGA1_c11490 0.19 +1.5 1,187,515 + PGA1_c11490 0.19 +0.7 1,187,518 - PGA1_c11490 0.19 +0.5 1,187,523 - PGA1_c11490 0.20 +0.7 1,187,542 + PGA1_c11490 0.22 -0.6 1,187,595 - PGA1_c11490 0.27 -0.0 1,187,666 - PGA1_c11490 0.35 -0.2 1,187,707 + PGA1_c11490 0.39 +0.3 1,187,725 + PGA1_c11490 0.41 -0.5 1,187,725 + PGA1_c11490 0.41 +0.7 1,187,735 - PGA1_c11490 0.42 +0.9 1,187,740 - PGA1_c11490 0.43 -0.4 1,187,740 - PGA1_c11490 0.43 +0.5
Or see this region's nucleotide sequence