Experiment: MRS + kanamycin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14015 and BBR_RS14020 are separated by 105 nucleotides BBR_RS14020 and BBR_RS20815 are separated by 67 nucleotides BBR_RS20815 and BBR_RS14025 are separated by 225 nucleotides
BBR_RS14015: BBR_RS14015 - hypothetical protein, at 921,135 to 921,809
_RS14015
BBR_RS14020: BBR_RS14020 - TrkA family potassium uptake protein, at 921,915 to 922,337
_RS14020
BBR_RS20815: BBR_RS20815 - potassium transporter Trk, at 922,405 to 922,824
_RS20815
BBR_RS14025: BBR_RS14025 - NAD kinase, at 923,050 to 924,030
_RS14025
Position (kb) 922
923 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 921.430 kb on + strand, within BBR_RS14015 at 921.430 kb on + strand, within BBR_RS14015 at 921.436 kb on + strand, within BBR_RS14015 at 921.528 kb on + strand, within BBR_RS14015 at 921.637 kb on + strand, within BBR_RS14015 at 921.759 kb on - strand at 921.777 kb on + strand at 921.869 kb on - strand at 921.918 kb on + strand at 921.992 kb on + strand, within BBR_RS14020 at 921.992 kb on + strand, within BBR_RS14020 at 922.049 kb on - strand, within BBR_RS14020 at 922.073 kb on + strand, within BBR_RS14020 at 922.121 kb on + strand, within BBR_RS14020 at 922.204 kb on + strand, within BBR_RS14020 at 922.217 kb on - strand, within BBR_RS14020 at 922.225 kb on + strand, within BBR_RS14020 at 922.238 kb on + strand, within BBR_RS14020 at 922.277 kb on + strand, within BBR_RS14020 at 922.384 kb on + strand at 922.429 kb on + strand at 922.437 kb on + strand at 922.503 kb on - strand, within BBR_RS20815 at 922.528 kb on + strand, within BBR_RS20815 at 922.581 kb on - strand, within BBR_RS20815 at 922.624 kb on - strand, within BBR_RS20815 at 922.648 kb on + strand, within BBR_RS20815 at 922.665 kb on - strand, within BBR_RS20815 at 922.738 kb on - strand, within BBR_RS20815 at 922.742 kb on - strand, within BBR_RS20815 at 922.754 kb on + strand, within BBR_RS20815 at 922.814 kb on - strand at 922.832 kb on + strand at 922.832 kb on + strand at 922.847 kb on - strand at 922.851 kb on + strand at 922.851 kb on + strand at 922.859 kb on + strand at 922.859 kb on - strand at 922.903 kb on + strand at 922.903 kb on + strand at 922.903 kb on + strand at 922.911 kb on + strand at 922.911 kb on - strand at 922.920 kb on + strand at 922.964 kb on + strand at 922.995 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + kanamycin Time48hr remove 921,430 + BBR_RS14015 0.44 -1.7 921,430 + BBR_RS14015 0.44 -0.7 921,436 + BBR_RS14015 0.45 -0.3 921,528 + BBR_RS14015 0.58 +0.3 921,637 + BBR_RS14015 0.74 +0.3 921,759 - -0.9 921,777 + -0.0 921,869 - -1.0 921,918 + -0.7 921,992 + BBR_RS14020 0.18 -1.2 921,992 + BBR_RS14020 0.18 +0.3 922,049 - BBR_RS14020 0.32 +3.7 922,073 + BBR_RS14020 0.37 -1.6 922,121 + BBR_RS14020 0.49 -1.6 922,204 + BBR_RS14020 0.68 -0.7 922,217 - BBR_RS14020 0.71 +3.6 922,225 + BBR_RS14020 0.73 -0.3 922,238 + BBR_RS14020 0.76 +1.0 922,277 + BBR_RS14020 0.86 +0.3 922,384 + +0.3 922,429 + -1.7 922,437 + +1.3 922,503 - BBR_RS20815 0.23 +1.3 922,528 + BBR_RS20815 0.29 +1.5 922,581 - BBR_RS20815 0.42 +1.0 922,624 - BBR_RS20815 0.52 +0.3 922,648 + BBR_RS20815 0.58 -1.0 922,665 - BBR_RS20815 0.62 +1.9 922,738 - BBR_RS20815 0.79 -0.1 922,742 - BBR_RS20815 0.80 +1.9 922,754 + BBR_RS20815 0.83 +0.9 922,814 - +1.5 922,832 + -1.6 922,832 + +2.3 922,847 - +1.1 922,851 + -0.7 922,851 + +3.3 922,859 + +1.0 922,859 - -3.0 922,903 + +1.3 922,903 + +0.7 922,903 + -0.7 922,911 + +0.7 922,911 - +2.0 922,920 + +0.3 922,964 + -1.3 922,995 - -0.0
Or see this region's nucleotide sequence