Strain Fitness in Bifidobacterium breve UCC2003 around BBR_RS17525

Experiment: MRS + kanamycin Time48hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	MRS + kanamycin Time48hr
1,708,007	+				-1.3
1,708,007	+				+1.5
1,708,017	-				-1.5
1,708,017	-				-2.1
1,708,103	+		BBR_RS17515	0.18	+2.6
1,708,103	+		BBR_RS17515	0.18	+1.0
1,708,103	+		BBR_RS17515	0.18	-1.1
1,708,103	+		BBR_RS17515	0.18	-2.1
1,708,111	-		BBR_RS17515	0.19	+0.3
1,708,111	-		BBR_RS17515	0.19	+0.3
1,708,169	-		BBR_RS17515	0.29	+1.3
1,708,203	-		BBR_RS17515	0.35	-0.5
1,708,213	-		BBR_RS17515	0.37	+0.3
1,708,213	-		BBR_RS17515	0.37	-0.3
1,708,242	+		BBR_RS17515	0.42	-2.5
1,708,242	+		BBR_RS17515	0.42	+0.6
1,708,242	+		BBR_RS17515	0.42	+0.3
1,708,310	+		BBR_RS17515	0.54	+0.3
1,708,310	+		BBR_RS17515	0.54	+0.3
1,708,318	-		BBR_RS17515	0.55	+2.7
1,708,318	-		BBR_RS17515	0.55	+0.3
1,708,331	+		BBR_RS17515	0.58	-2.0
1,708,341	+		BBR_RS17515	0.60	-0.7
1,708,369	+		BBR_RS17515	0.64	-1.5
1,708,377	-		BBR_RS17515	0.66	-0.7
1,708,377	-		BBR_RS17515	0.66	+0.3
1,708,377	-		BBR_RS17515	0.66	+1.3
1,708,377	-		BBR_RS17515	0.66	+0.3
1,708,382	-		BBR_RS17515	0.67	+1.3
1,708,392	-		BBR_RS17515	0.68	-1.1
1,708,418	-		BBR_RS17515	0.73	+1.3
1,708,484	-		BBR_RS17515	0.84	-0.6
1,708,564	-				+1.3
1,708,564	-				+0.3
1,708,577	-				+1.4
1,708,612	+				-3.3
1,708,612	+				-2.7
1,708,645	-				+0.3
1,708,654	-				+2.9
1,708,660	+				+0.9
1,708,683	-				+2.6
1,708,684	-				+0.3
1,708,691	-				+0.3
1,708,708	+				+2.9
1,708,718	+				-0.5
1,708,727	+				+1.2
1,708,800	-		BBR_RS17520	0.33	-1.2
1,708,810	-		BBR_RS17520	0.36	+1.3
1,708,810	-		BBR_RS17520	0.36	-0.7
1,708,827	+		BBR_RS17520	0.41	+1.9
1,708,827	+		BBR_RS17520	0.41	-0.7
1,708,836	-		BBR_RS17520	0.44	+0.3
1,708,883	+		BBR_RS17520	0.60	+1.3
1,708,893	+		BBR_RS17520	0.63	+0.7
1,708,962	-		BBR_RS17520	0.85	+0.3
1,708,963	+		BBR_RS17520	0.85	+0.9
1,708,975	-		BBR_RS17520	0.89	-1.9
1,708,998	-				+0.7
1,709,069	+				+1.9
1,709,076	+				-0.1
1,709,145	-				+2.5
1,709,190	+		BBR_RS17525	0.12	-0.2
1,709,190	+		BBR_RS17525	0.12	-0.8
1,709,206	-		BBR_RS17525	0.13	+0.3
1,709,224	-		BBR_RS17525	0.14	+0.3
1,709,231	+		BBR_RS17525	0.14	-2.1
1,709,269	-		BBR_RS17525	0.17	+0.3
1,709,278	+		BBR_RS17525	0.17	-1.9
1,709,286	-		BBR_RS17525	0.18	+2.3
1,709,403	+		BBR_RS17525	0.25	+1.9
1,709,411	+		BBR_RS17525	0.26	-0.5
1,709,419	-		BBR_RS17525	0.26	+1.3
1,709,423	+		BBR_RS17525	0.27	-1.1
1,709,450	-		BBR_RS17525	0.28	-1.5
1,709,512	-		BBR_RS17525	0.32	-3.1
1,709,522	-		BBR_RS17525	0.33	-0.7
1,709,530	-		BBR_RS17525	0.33	-2.7
1,709,590	+		BBR_RS17525	0.37	-1.2
1,709,590	+		BBR_RS17525	0.37	+0.3
1,709,598	-		BBR_RS17525	0.38	+0.3
1,709,608	-		BBR_RS17525	0.38	-2.3
1,709,663	+		BBR_RS17525	0.42	-2.8
1,709,689	+		BBR_RS17525	0.43	+1.3
1,709,697	+		BBR_RS17525	0.44	-0.3
1,709,706	+		BBR_RS17525	0.44	-1.3
1,709,760	-		BBR_RS17525	0.48	-1.6
1,709,823	-		BBR_RS17525	0.52	-1.3
1,709,828	-		BBR_RS17525	0.52	+0.3
1,709,846	-		BBR_RS17525	0.53	+0.3
1,709,867	-		BBR_RS17525	0.55	+2.6
1,709,923	+		BBR_RS17525	0.58	-1.3
1,709,953	+		BBR_RS17525	0.60	+1.3
1,709,961	-		BBR_RS17525	0.61	-2.1
1,709,971	+		BBR_RS17525	0.61	+1.3
1,710,029	+		BBR_RS17525	0.65	-2.1
1,710,095	-		BBR_RS17525	0.69	+0.3
1,710,097	+		BBR_RS17525	0.69	-1.7
1,710,118	+		BBR_RS17525	0.70	-0.3
1,710,135	+		BBR_RS17525	0.72	-2.7
1,710,178	-		BBR_RS17525	0.74	-1.7
1,710,222	+		BBR_RS17525	0.77	+1.6
1,710,222	+		BBR_RS17525	0.77	-2.5
1,710,231	-		BBR_RS17525	0.78	+1.0
1,710,236	+		BBR_RS17525	0.78	+1.3
1,710,242	+		BBR_RS17525	0.78	+0.3
1,710,269	+		BBR_RS17525	0.80	-1.0
1,710,269	+		BBR_RS17525	0.80	+1.9
1,710,270	+		BBR_RS17525	0.80	+3.1
1,710,277	-		BBR_RS17525	0.81	+2.1
1,710,282	-		BBR_RS17525	0.81	-1.7
1,710,301	-		BBR_RS17525	0.82	+0.3
1,710,317	+		BBR_RS17525	0.83	-2.3
1,710,325	-		BBR_RS17525	0.84	-1.4
1,710,336	-		BBR_RS17525	0.84	-0.6
1,710,356	-		BBR_RS17525	0.86	-0.7
1,710,374	+		BBR_RS17525	0.87	+0.6
1,710,374	+		BBR_RS17525	0.87	-2.1
1,710,374	+		BBR_RS17525	0.87	-0.5
1,710,377	-		BBR_RS17525	0.87	+0.3
1,710,515	+				+1.0
1,710,529	+				+1.1
1,710,529	+				-2.1
1,710,560	+				+0.3
1,710,570	-				-0.7
1,710,580	+				+0.3
1,710,605	-				+0.3
1,710,618	-				+0.6
1,710,705	+		BBR_RS17530	0.15	-3.6
1,710,710	+		BBR_RS17530	0.15	+1.0
1,710,710	+		BBR_RS17530	0.15	-0.8
1,710,713	-		BBR_RS17530	0.16	+0.3
1,710,713	-		BBR_RS17530	0.16	-0.8
1,710,740	+		BBR_RS17530	0.20	+0.5
1,710,740	+		BBR_RS17530	0.20	+0.9
1,710,777	+		BBR_RS17530	0.26	-0.7
1,710,783	-		BBR_RS17530	0.27	+0.4
1,710,785	+		BBR_RS17530	0.27	-1.3
1,710,801	-		BBR_RS17530	0.29	-0.7
1,710,807	-		BBR_RS17530	0.30	+1.9
1,710,819	-		BBR_RS17530	0.32	-0.0
1,710,829	+		BBR_RS17530	0.34	-0.3
1,710,853	+		BBR_RS17530	0.38	+1.3
1,710,903	+		BBR_RS17530	0.45	+1.9
1,710,910	-		BBR_RS17530	0.46	-1.2
1,710,971	+		BBR_RS17530	0.56	-0.1
1,710,983	-		BBR_RS17530	0.58	+0.3
1,711,043	+		BBR_RS17530	0.67	+0.8
1,711,067	+		BBR_RS17530	0.71	-0.5
1,711,128	-		BBR_RS17530	0.80	-0.3
1,711,153	+		BBR_RS17530	0.84	-0.1
1,711,159	+		BBR_RS17530	0.85	+0.3
1,711,167	-		BBR_RS17530	0.86	+0.3
1,711,172	-		BBR_RS17530	0.87	+0.7
1,711,220	+				-0.9
1,711,228	-				+0.3
1,711,236	+				-3.5
1,711,257	+				-3.4
1,711,268	+				-1.1
1,711,268	+				+2.4
1,711,270	+				-2.2
1,711,283	-				+0.4
1,711,285	+				+0.3
1,711,285	-				+1.3
1,711,294	+				+0.0
1,711,302	-				+0.7
1,711,306	+				+0.3
1,711,306	+				-2.9
1,711,314	-				-0.6
1,711,314	-				+0.3
1,711,324	-				+1.3
1,711,342	-				-0.7
1,711,352	-				-1.1
1,711,352	-				-0.8
1,711,352	-				+0.9
1,711,367	-				+1.3
1,711,367	-				-2.9
1,711,367	-				-2.4
1,711,370	+				-0.6