Experiment: MRS + kanamycin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15930 and BBR_RS15935 are separated by 33 nucleotides BBR_RS15935 and BBR_RS15940 are separated by 131 nucleotides BBR_RS15940 and BBR_RS15945 overlap by 4 nucleotides BBR_RS15945 and BBR_RS15950 are separated by 51 nucleotides
BBR_RS15930: BBR_RS15930 - RNA polymerase sigma factor, at 1,334,022 to 1,334,573
_RS15930
BBR_RS15935: BBR_RS15935 - hypothetical protein, at 1,334,607 to 1,335,434
_RS15935
BBR_RS15940: BBR_RS15940 - hypothetical protein, at 1,335,566 to 1,336,192
_RS15940
BBR_RS15945: BBR_RS15945 - hypothetical protein, at 1,336,189 to 1,336,971
_RS15945
BBR_RS15950: BBR_RS15950 - ABC transporter ATP-binding protein, at 1,337,023 to 1,337,898
_RS15950
Position (kb)
1335
1336
1337 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1334.608 kb on + strand at 1334.764 kb on - strand, within BBR_RS15935 at 1334.768 kb on - strand, within BBR_RS15935 at 1334.794 kb on + strand, within BBR_RS15935 at 1334.804 kb on + strand, within BBR_RS15935 at 1334.836 kb on - strand, within BBR_RS15935 at 1334.853 kb on + strand, within BBR_RS15935 at 1334.863 kb on + strand, within BBR_RS15935 at 1334.928 kb on + strand, within BBR_RS15935 at 1334.933 kb on + strand, within BBR_RS15935 at 1334.947 kb on + strand, within BBR_RS15935 at 1334.999 kb on + strand, within BBR_RS15935 at 1335.027 kb on + strand, within BBR_RS15935 at 1335.105 kb on - strand, within BBR_RS15935 at 1335.105 kb on - strand, within BBR_RS15935 at 1335.105 kb on - strand, within BBR_RS15935 at 1335.138 kb on - strand, within BBR_RS15935 at 1335.138 kb on - strand, within BBR_RS15935 at 1335.153 kb on - strand, within BBR_RS15935 at 1335.164 kb on + strand, within BBR_RS15935 at 1335.190 kb on - strand, within BBR_RS15935 at 1335.278 kb on + strand, within BBR_RS15935 at 1335.324 kb on - strand, within BBR_RS15935 at 1335.397 kb on - strand at 1335.401 kb on + strand at 1335.445 kb on + strand at 1335.483 kb on + strand at 1335.589 kb on + strand at 1335.589 kb on + strand at 1335.589 kb on + strand at 1335.589 kb on + strand at 1335.597 kb on - strand at 1335.597 kb on - strand at 1335.623 kb on + strand at 1335.657 kb on + strand, within BBR_RS15940 at 1335.701 kb on + strand, within BBR_RS15940 at 1335.735 kb on + strand, within BBR_RS15940 at 1335.748 kb on - strand, within BBR_RS15940 at 1335.793 kb on + strand, within BBR_RS15940 at 1335.806 kb on + strand, within BBR_RS15940 at 1335.814 kb on - strand, within BBR_RS15940 at 1335.894 kb on + strand, within BBR_RS15940 at 1335.932 kb on - strand, within BBR_RS15940 at 1335.932 kb on - strand, within BBR_RS15940 at 1336.144 kb on + strand at 1336.193 kb on - strand at 1336.302 kb on - strand, within BBR_RS15945 at 1336.413 kb on + strand, within BBR_RS15945 at 1336.413 kb on + strand, within BBR_RS15945 at 1336.413 kb on + strand, within BBR_RS15945 at 1336.431 kb on - strand, within BBR_RS15945 at 1336.431 kb on - strand, within BBR_RS15945 at 1336.450 kb on + strand, within BBR_RS15945 at 1336.450 kb on + strand, within BBR_RS15945 at 1336.466 kb on - strand, within BBR_RS15945 at 1336.466 kb on - strand, within BBR_RS15945 at 1336.477 kb on + strand, within BBR_RS15945 at 1336.623 kb on + strand, within BBR_RS15945 at 1336.633 kb on + strand, within BBR_RS15945 at 1336.726 kb on + strand, within BBR_RS15945 at 1336.905 kb on + strand at 1336.915 kb on + strand at 1336.935 kb on - strand at 1336.941 kb on + strand at 1336.941 kb on + strand at 1336.949 kb on - strand at 1336.953 kb on + strand at 1336.959 kb on - strand at 1336.961 kb on - strand at 1336.970 kb on + strand at 1337.090 kb on + strand at 1337.106 kb on + strand at 1337.186 kb on + strand, within BBR_RS15950
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + kanamycin Time48hr remove 1,334,608 + -1.9 1,334,764 - BBR_RS15935 0.19 -1.9 1,334,768 - BBR_RS15935 0.19 -0.5 1,334,794 + BBR_RS15935 0.23 -1.6 1,334,804 + BBR_RS15935 0.24 -1.5 1,334,836 - BBR_RS15935 0.28 -0.7 1,334,853 + BBR_RS15935 0.30 -3.3 1,334,863 + BBR_RS15935 0.31 +0.3 1,334,928 + BBR_RS15935 0.39 -1.3 1,334,933 + BBR_RS15935 0.39 -2.0 1,334,947 + BBR_RS15935 0.41 +1.9 1,334,999 + BBR_RS15935 0.47 +0.3 1,335,027 + BBR_RS15935 0.51 +2.3 1,335,105 - BBR_RS15935 0.60 +0.3 1,335,105 - BBR_RS15935 0.60 +1.9 1,335,105 - BBR_RS15935 0.60 -2.3 1,335,138 - BBR_RS15935 0.64 +2.3 1,335,138 - BBR_RS15935 0.64 -0.1 1,335,153 - BBR_RS15935 0.66 -1.3 1,335,164 + BBR_RS15935 0.67 +2.1 1,335,190 - BBR_RS15935 0.70 +0.3 1,335,278 + BBR_RS15935 0.81 -1.0 1,335,324 - BBR_RS15935 0.87 +0.0 1,335,397 - +0.7 1,335,401 + +1.6 1,335,445 + -0.8 1,335,483 + +0.3 1,335,589 + -0.8 1,335,589 + -0.4 1,335,589 + +0.9 1,335,589 + -3.2 1,335,597 - +0.3 1,335,597 - +0.3 1,335,623 + -1.2 1,335,657 + BBR_RS15940 0.15 +0.3 1,335,701 + BBR_RS15940 0.22 -0.4 1,335,735 + BBR_RS15940 0.27 +2.4 1,335,748 - BBR_RS15940 0.29 +0.6 1,335,793 + BBR_RS15940 0.36 +0.3 1,335,806 + BBR_RS15940 0.38 +0.4 1,335,814 - BBR_RS15940 0.40 -2.0 1,335,894 + BBR_RS15940 0.52 +0.5 1,335,932 - BBR_RS15940 0.58 -4.1 1,335,932 - BBR_RS15940 0.58 +1.3 1,336,144 + -1.3 1,336,193 - -0.9 1,336,302 - BBR_RS15945 0.14 +2.9 1,336,413 + BBR_RS15945 0.29 +0.3 1,336,413 + BBR_RS15945 0.29 -0.7 1,336,413 + BBR_RS15945 0.29 +0.1 1,336,431 - BBR_RS15945 0.31 -0.7 1,336,431 - BBR_RS15945 0.31 -3.1 1,336,450 + BBR_RS15945 0.33 +2.9 1,336,450 + BBR_RS15945 0.33 -2.3 1,336,466 - BBR_RS15945 0.35 -0.1 1,336,466 - BBR_RS15945 0.35 +2.0 1,336,477 + BBR_RS15945 0.37 +2.0 1,336,623 + BBR_RS15945 0.55 +1.3 1,336,633 + BBR_RS15945 0.57 +1.9 1,336,726 + BBR_RS15945 0.69 -2.5 1,336,905 + -0.6 1,336,915 + -2.0 1,336,935 - +0.3 1,336,941 + +4.6 1,336,941 + +1.0 1,336,949 - -3.4 1,336,953 + -1.8 1,336,959 - -1.7 1,336,961 - -0.2 1,336,970 + -0.7 1,337,090 + -0.7 1,337,106 + -0.7 1,337,186 + BBR_RS15950 0.19 +0.3
Or see this region's nucleotide sequence