Experiment: MRS + kanamycin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14635 and BBR_RS14640 overlap by 8 nucleotides BBR_RS14640 and BBR_RS14645 overlap by 23 nucleotides BBR_RS14645 and BBR_RS14650 are separated by 441 nucleotides
BBR_RS14635: BBR_RS14635 - amino acid ABC transporter ATP-binding protein, at 1,059,355 to 1,060,143
_RS14635
BBR_RS14640: BBR_RS14640 - amino acid ABC transporter permease, at 1,060,136 to 1,060,843
_RS14640
BBR_RS14645: BBR_RS14645 - amino acid ABC transporter permease, at 1,060,821 to 1,061,483
_RS14645
BBR_RS14650: BBR_RS14650 - redox-regulated ATPase YchF, at 1,061,925 to 1,063,025
_RS14650
Position (kb)
1060
1061
1062 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1059.834 kb on + strand, within BBR_RS14635 at 1059.852 kb on - strand, within BBR_RS14635 at 1059.915 kb on - strand, within BBR_RS14635 at 1059.925 kb on + strand, within BBR_RS14635 at 1060.051 kb on - strand, within BBR_RS14635 at 1060.143 kb on + strand at 1060.161 kb on - strand at 1060.166 kb on + strand at 1060.169 kb on - strand at 1060.175 kb on - strand at 1060.335 kb on - strand, within BBR_RS14640 at 1060.360 kb on + strand, within BBR_RS14640 at 1060.465 kb on - strand, within BBR_RS14640 at 1060.554 kb on + strand, within BBR_RS14640 at 1060.562 kb on - strand, within BBR_RS14640 at 1060.604 kb on + strand, within BBR_RS14640 at 1060.617 kb on - strand, within BBR_RS14640 at 1060.634 kb on - strand, within BBR_RS14640 at 1060.666 kb on + strand, within BBR_RS14640 at 1060.666 kb on + strand, within BBR_RS14640 at 1060.681 kb on + strand, within BBR_RS14640 at 1060.744 kb on + strand, within BBR_RS14640 at 1061.011 kb on - strand, within BBR_RS14645 at 1061.013 kb on + strand, within BBR_RS14645 at 1061.013 kb on + strand, within BBR_RS14645 at 1061.058 kb on + strand, within BBR_RS14645 at 1061.104 kb on + strand, within BBR_RS14645 at 1061.181 kb on + strand, within BBR_RS14645 at 1061.216 kb on - strand, within BBR_RS14645 at 1061.231 kb on - strand, within BBR_RS14645 at 1061.323 kb on + strand, within BBR_RS14645 at 1061.331 kb on - strand, within BBR_RS14645 at 1061.352 kb on - strand, within BBR_RS14645 at 1061.406 kb on - strand, within BBR_RS14645 at 1061.448 kb on - strand at 1061.483 kb on + strand at 1061.486 kb on - strand at 1061.505 kb on - strand at 1061.558 kb on + strand at 1061.572 kb on + strand at 1061.587 kb on + strand at 1061.594 kb on + strand at 1061.656 kb on - strand at 1061.664 kb on - strand at 1061.826 kb on - strand at 1061.917 kb on - strand at 1062.095 kb on + strand, within BBR_RS14650
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + kanamycin Time48hr remove 1,059,834 + BBR_RS14635 0.61 +0.3 1,059,852 - BBR_RS14635 0.63 -1.3 1,059,915 - BBR_RS14635 0.71 -0.7 1,059,925 + BBR_RS14635 0.72 +0.3 1,060,051 - BBR_RS14635 0.88 +0.3 1,060,143 + -0.4 1,060,161 - -0.7 1,060,166 + -2.6 1,060,169 - -0.1 1,060,175 - -0.3 1,060,335 - BBR_RS14640 0.28 -0.3 1,060,360 + BBR_RS14640 0.32 -0.7 1,060,465 - BBR_RS14640 0.46 -2.0 1,060,554 + BBR_RS14640 0.59 -3.6 1,060,562 - BBR_RS14640 0.60 -1.0 1,060,604 + BBR_RS14640 0.66 -0.7 1,060,617 - BBR_RS14640 0.68 -1.9 1,060,634 - BBR_RS14640 0.70 -3.0 1,060,666 + BBR_RS14640 0.75 +0.3 1,060,666 + BBR_RS14640 0.75 -1.7 1,060,681 + BBR_RS14640 0.77 -0.7 1,060,744 + BBR_RS14640 0.86 +0.3 1,061,011 - BBR_RS14645 0.29 +0.3 1,061,013 + BBR_RS14645 0.29 -2.9 1,061,013 + BBR_RS14645 0.29 -2.9 1,061,058 + BBR_RS14645 0.36 +0.3 1,061,104 + BBR_RS14645 0.43 -1.3 1,061,181 + BBR_RS14645 0.54 -3.4 1,061,216 - BBR_RS14645 0.60 +0.9 1,061,231 - BBR_RS14645 0.62 +0.3 1,061,323 + BBR_RS14645 0.76 -2.7 1,061,331 - BBR_RS14645 0.77 -2.0 1,061,352 - BBR_RS14645 0.80 -2.3 1,061,406 - BBR_RS14645 0.88 +0.3 1,061,448 - +0.3 1,061,483 + -1.0 1,061,486 - +0.3 1,061,505 - -1.7 1,061,558 + +3.5 1,061,572 + -0.9 1,061,587 + +0.3 1,061,594 + -2.0 1,061,656 - -2.5 1,061,664 - -3.0 1,061,826 - +0.3 1,061,917 - +0.9 1,062,095 + BBR_RS14650 0.15 +0.3
Or see this region's nucleotide sequence