Experiment: MRS + kanamycin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14560 and BBR_RS14565 are separated by 101 nucleotides BBR_RS14565 and BBR_RS14570 overlap by 4 nucleotides
BBR_RS14560: BBR_RS14560 - FAD-binding protein, at 1,038,703 to 1,040,565
_RS14560
BBR_RS14565: BBR_RS14565 - DNA-processing protein DprA, at 1,040,667 to 1,042,286
_RS14565
BBR_RS14570: BBR_RS14570 - ATP-binding protein, at 1,042,283 to 1,043,818
_RS14570
Position (kb)
1040
1041
1042
1043 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1040.641 kb on + strand at 1040.641 kb on + strand at 1040.643 kb on + strand at 1040.643 kb on + strand at 1040.643 kb on + strand at 1040.651 kb on - strand at 1040.651 kb on - strand at 1040.651 kb on - strand at 1040.651 kb on - strand at 1040.675 kb on + strand at 1040.693 kb on - strand at 1040.734 kb on + strand at 1040.742 kb on - strand at 1040.773 kb on + strand at 1040.808 kb on + strand at 1040.818 kb on - strand at 1040.822 kb on + strand at 1040.824 kb on - strand at 1040.887 kb on + strand, within BBR_RS14565 at 1040.887 kb on + strand, within BBR_RS14565 at 1040.892 kb on + strand, within BBR_RS14565 at 1040.900 kb on - strand, within BBR_RS14565 at 1040.979 kb on + strand, within BBR_RS14565 at 1041.015 kb on - strand, within BBR_RS14565 at 1041.027 kb on - strand, within BBR_RS14565 at 1041.036 kb on - strand, within BBR_RS14565 at 1041.058 kb on + strand, within BBR_RS14565 at 1041.080 kb on - strand, within BBR_RS14565 at 1041.116 kb on + strand, within BBR_RS14565 at 1041.124 kb on - strand, within BBR_RS14565 at 1041.162 kb on - strand, within BBR_RS14565 at 1041.183 kb on + strand, within BBR_RS14565 at 1041.198 kb on - strand, within BBR_RS14565 at 1041.251 kb on + strand, within BBR_RS14565 at 1041.262 kb on + strand, within BBR_RS14565 at 1041.270 kb on - strand, within BBR_RS14565 at 1041.277 kb on - strand, within BBR_RS14565 at 1041.288 kb on + strand, within BBR_RS14565 at 1041.288 kb on + strand, within BBR_RS14565 at 1041.298 kb on + strand, within BBR_RS14565 at 1041.334 kb on + strand, within BBR_RS14565 at 1041.334 kb on + strand, within BBR_RS14565 at 1041.336 kb on + strand, within BBR_RS14565 at 1041.344 kb on + strand, within BBR_RS14565 at 1041.380 kb on + strand, within BBR_RS14565 at 1041.454 kb on + strand, within BBR_RS14565 at 1041.464 kb on + strand, within BBR_RS14565 at 1041.568 kb on + strand, within BBR_RS14565 at 1041.629 kb on - strand, within BBR_RS14565 at 1041.649 kb on + strand, within BBR_RS14565 at 1041.720 kb on + strand, within BBR_RS14565 at 1041.720 kb on + strand, within BBR_RS14565 at 1041.720 kb on + strand, within BBR_RS14565 at 1041.752 kb on + strand, within BBR_RS14565 at 1041.753 kb on + strand, within BBR_RS14565 at 1041.753 kb on + strand, within BBR_RS14565 at 1041.753 kb on + strand, within BBR_RS14565 at 1041.753 kb on + strand, within BBR_RS14565 at 1041.805 kb on + strand, within BBR_RS14565 at 1041.805 kb on + strand, within BBR_RS14565 at 1041.918 kb on + strand, within BBR_RS14565 at 1041.983 kb on - strand, within BBR_RS14565 at 1042.011 kb on - strand, within BBR_RS14565 at 1042.016 kb on - strand, within BBR_RS14565 at 1042.016 kb on - strand, within BBR_RS14565 at 1042.053 kb on - strand, within BBR_RS14565 at 1042.057 kb on + strand, within BBR_RS14565 at 1042.083 kb on - strand, within BBR_RS14565 at 1042.083 kb on - strand, within BBR_RS14565 at 1042.093 kb on - strand, within BBR_RS14565 at 1042.101 kb on - strand, within BBR_RS14565 at 1042.102 kb on - strand, within BBR_RS14565 at 1042.137 kb on + strand at 1042.155 kb on + strand at 1042.155 kb on + strand at 1042.155 kb on + strand at 1042.155 kb on + strand at 1042.155 kb on + strand at 1042.163 kb on - strand at 1042.163 kb on - strand at 1042.163 kb on - strand at 1042.163 kb on - strand at 1042.163 kb on - strand at 1042.235 kb on + strand at 1042.281 kb on + strand at 1042.289 kb on - strand at 1042.311 kb on + strand at 1042.364 kb on + strand at 1042.402 kb on + strand at 1042.436 kb on - strand at 1042.455 kb on - strand, within BBR_RS14570 at 1042.460 kb on - strand, within BBR_RS14570 at 1042.531 kb on - strand, within BBR_RS14570 at 1042.531 kb on - strand, within BBR_RS14570 at 1042.602 kb on + strand, within BBR_RS14570 at 1042.703 kb on + strand, within BBR_RS14570 at 1042.805 kb on + strand, within BBR_RS14570 at 1042.889 kb on - strand, within BBR_RS14570 at 1042.889 kb on - strand, within BBR_RS14570 at 1043.017 kb on - strand, within BBR_RS14570 at 1043.025 kb on + strand, within BBR_RS14570 at 1043.033 kb on - strand, within BBR_RS14570 at 1043.062 kb on + strand, within BBR_RS14570 at 1043.099 kb on + strand, within BBR_RS14570 at 1043.139 kb on + strand, within BBR_RS14570 at 1043.141 kb on - strand, within BBR_RS14570 at 1043.148 kb on + strand, within BBR_RS14570 at 1043.221 kb on - strand, within BBR_RS14570
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + kanamycin Time48hr remove 1,040,641 + +0.8 1,040,641 + +0.8 1,040,643 + +0.3 1,040,643 + +0.1 1,040,643 + +1.9 1,040,651 - -2.3 1,040,651 - +0.5 1,040,651 - -0.3 1,040,651 - -0.1 1,040,675 + -0.7 1,040,693 - +2.3 1,040,734 + -2.0 1,040,742 - +1.3 1,040,773 + -4.9 1,040,808 + -0.0 1,040,818 - -0.7 1,040,822 + +0.3 1,040,824 - -3.0 1,040,887 + BBR_RS14565 0.14 -2.6 1,040,887 + BBR_RS14565 0.14 -2.0 1,040,892 + BBR_RS14565 0.14 -0.7 1,040,900 - BBR_RS14565 0.14 +0.9 1,040,979 + BBR_RS14565 0.19 -1.3 1,041,015 - BBR_RS14565 0.21 -0.3 1,041,027 - BBR_RS14565 0.22 -1.3 1,041,036 - BBR_RS14565 0.23 -2.3 1,041,058 + BBR_RS14565 0.24 +0.7 1,041,080 - BBR_RS14565 0.25 +1.2 1,041,116 + BBR_RS14565 0.28 +0.3 1,041,124 - BBR_RS14565 0.28 +1.7 1,041,162 - BBR_RS14565 0.31 +1.3 1,041,183 + BBR_RS14565 0.32 -0.1 1,041,198 - BBR_RS14565 0.33 +1.3 1,041,251 + BBR_RS14565 0.36 -1.3 1,041,262 + BBR_RS14565 0.37 +3.3 1,041,270 - BBR_RS14565 0.37 -0.7 1,041,277 - BBR_RS14565 0.38 -2.3 1,041,288 + BBR_RS14565 0.38 +0.7 1,041,288 + BBR_RS14565 0.38 +3.5 1,041,298 + BBR_RS14565 0.39 -0.4 1,041,334 + BBR_RS14565 0.41 +1.9 1,041,334 + BBR_RS14565 0.41 -2.7 1,041,336 + BBR_RS14565 0.41 -2.7 1,041,344 + BBR_RS14565 0.42 +0.3 1,041,380 + BBR_RS14565 0.44 +0.3 1,041,454 + BBR_RS14565 0.49 -0.7 1,041,464 + BBR_RS14565 0.49 -1.7 1,041,568 + BBR_RS14565 0.56 +2.7 1,041,629 - BBR_RS14565 0.59 +0.3 1,041,649 + BBR_RS14565 0.61 +1.0 1,041,720 + BBR_RS14565 0.65 +0.7 1,041,720 + BBR_RS14565 0.65 +1.0 1,041,720 + BBR_RS14565 0.65 +0.6 1,041,752 + BBR_RS14565 0.67 +0.9 1,041,753 + BBR_RS14565 0.67 -0.4 1,041,753 + BBR_RS14565 0.67 -0.4 1,041,753 + BBR_RS14565 0.67 -1.0 1,041,753 + BBR_RS14565 0.67 +2.0 1,041,805 + BBR_RS14565 0.70 +0.8 1,041,805 + BBR_RS14565 0.70 -1.3 1,041,918 + BBR_RS14565 0.77 -0.7 1,041,983 - BBR_RS14565 0.81 +0.3 1,042,011 - BBR_RS14565 0.83 +2.0 1,042,016 - BBR_RS14565 0.83 -0.3 1,042,016 - BBR_RS14565 0.83 +0.3 1,042,053 - BBR_RS14565 0.86 -0.7 1,042,057 + BBR_RS14565 0.86 +0.6 1,042,083 - BBR_RS14565 0.87 -3.4 1,042,083 - BBR_RS14565 0.87 +0.3 1,042,093 - BBR_RS14565 0.88 +0.3 1,042,101 - BBR_RS14565 0.89 -1.7 1,042,102 - BBR_RS14565 0.89 +0.3 1,042,137 + +0.1 1,042,155 + -0.6 1,042,155 + +0.5 1,042,155 + +3.8 1,042,155 + -0.7 1,042,155 + -1.6 1,042,163 - +0.3 1,042,163 - -2.8 1,042,163 - -2.5 1,042,163 - -1.7 1,042,163 - -0.7 1,042,235 + -2.8 1,042,281 + -0.8 1,042,289 - +1.3 1,042,311 + +0.3 1,042,364 + -0.1 1,042,402 + -0.6 1,042,436 - -0.1 1,042,455 - BBR_RS14570 0.11 +0.3 1,042,460 - BBR_RS14570 0.12 +0.3 1,042,531 - BBR_RS14570 0.16 -0.7 1,042,531 - BBR_RS14570 0.16 +1.9 1,042,602 + BBR_RS14570 0.21 +0.7 1,042,703 + BBR_RS14570 0.27 -3.3 1,042,805 + BBR_RS14570 0.34 +1.0 1,042,889 - BBR_RS14570 0.39 -1.3 1,042,889 - BBR_RS14570 0.39 +1.9 1,043,017 - BBR_RS14570 0.48 -0.8 1,043,025 + BBR_RS14570 0.48 +0.3 1,043,033 - BBR_RS14570 0.49 -0.7 1,043,062 + BBR_RS14570 0.51 -1.3 1,043,099 + BBR_RS14570 0.53 +0.3 1,043,139 + BBR_RS14570 0.56 +1.7 1,043,141 - BBR_RS14570 0.56 +1.3 1,043,148 + BBR_RS14570 0.56 +0.6 1,043,221 - BBR_RS14570 0.61 -0.7
Or see this region's nucleotide sequence