Experiment: MRS + kanamycin Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12330 and BBR_RS12335 are separated by 140 nucleotides BBR_RS12335 and BBR_RS12340 are separated by 9 nucleotides BBR_RS12340 and BBR_RS12345 are separated by 167 nucleotides BBR_RS12345 and BBR_RS12350 are separated by 15 nucleotides
BBR_RS12330: BBR_RS12330 - ABC transporter ATP-binding protein, at 518,231 to 520,444
_RS12330
BBR_RS12335: BBR_RS12335 - PIN domain-containing protein, at 520,585 to 521,007
_RS12335
BBR_RS12340: BBR_RS12340 - Arc family DNA-binding protein, at 521,017 to 521,295
_RS12340
BBR_RS12345: BBR_RS12345 - acetolactate synthase small subunit, at 521,463 to 522,020
_RS12345
BBR_RS12350: BBR_RS12350 - acetolactate synthase large subunit, at 522,036 to 524,000
_RS12350
Position (kb) 521
522 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 520.021 kb on + strand, within BBR_RS12330 at 520.032 kb on - strand, within BBR_RS12330 at 520.032 kb on - strand, within BBR_RS12330 at 520.032 kb on - strand, within BBR_RS12330 at 520.032 kb on - strand, within BBR_RS12330 at 520.032 kb on - strand, within BBR_RS12330 at 520.044 kb on + strand, within BBR_RS12330 at 520.047 kb on - strand, within BBR_RS12330 at 520.052 kb on - strand, within BBR_RS12330 at 520.055 kb on + strand, within BBR_RS12330 at 520.055 kb on + strand, within BBR_RS12330 at 520.063 kb on + strand, within BBR_RS12330 at 520.090 kb on + strand, within BBR_RS12330 at 520.114 kb on - strand, within BBR_RS12330 at 520.123 kb on - strand, within BBR_RS12330 at 520.127 kb on + strand, within BBR_RS12330 at 520.140 kb on - strand, within BBR_RS12330 at 520.144 kb on + strand, within BBR_RS12330 at 520.144 kb on + strand, within BBR_RS12330 at 520.144 kb on + strand, within BBR_RS12330 at 520.144 kb on + strand, within BBR_RS12330 at 520.144 kb on + strand, within BBR_RS12330 at 520.144 kb on + strand, within BBR_RS12330 at 520.144 kb on + strand, within BBR_RS12330 at 520.144 kb on + strand, within BBR_RS12330 at 520.145 kb on + strand, within BBR_RS12330 at 520.145 kb on + strand, within BBR_RS12330 at 520.146 kb on - strand, within BBR_RS12330 at 520.152 kb on + strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.152 kb on - strand, within BBR_RS12330 at 520.160 kb on + strand, within BBR_RS12330 at 520.212 kb on + strand, within BBR_RS12330 at 520.220 kb on - strand, within BBR_RS12330 at 520.220 kb on - strand, within BBR_RS12330 at 520.246 kb on + strand at 520.252 kb on - strand at 520.254 kb on - strand at 520.326 kb on + strand at 520.326 kb on + strand at 520.326 kb on + strand at 520.326 kb on + strand at 520.326 kb on + strand at 520.334 kb on - strand at 520.334 kb on - strand at 520.334 kb on - strand at 520.334 kb on - strand at 520.334 kb on - strand at 520.334 kb on - strand at 520.385 kb on - strand at 520.416 kb on + strand at 520.424 kb on - strand at 520.433 kb on - strand at 520.457 kb on + strand at 520.457 kb on + strand at 520.457 kb on + strand at 520.465 kb on - strand at 520.497 kb on - strand at 520.500 kb on + strand at 520.530 kb on - strand at 520.530 kb on - strand at 520.531 kb on - strand at 520.550 kb on - strand at 520.557 kb on + strand at 520.560 kb on - strand at 520.582 kb on + strand at 520.582 kb on + strand at 520.582 kb on + strand at 520.582 kb on + strand at 520.590 kb on - strand at 520.607 kb on - strand at 520.609 kb on + strand at 520.653 kb on + strand, within BBR_RS12335 at 520.667 kb on + strand, within BBR_RS12335 at 520.675 kb on - strand, within BBR_RS12335 at 520.709 kb on + strand, within BBR_RS12335 at 520.801 kb on + strand, within BBR_RS12335 at 520.809 kb on - strand, within BBR_RS12335 at 520.814 kb on + strand, within BBR_RS12335 at 520.830 kb on - strand, within BBR_RS12335 at 520.830 kb on - strand, within BBR_RS12335 at 520.847 kb on - strand, within BBR_RS12335 at 520.858 kb on - strand, within BBR_RS12335 at 520.879 kb on - strand, within BBR_RS12335 at 520.891 kb on + strand, within BBR_RS12335 at 520.896 kb on + strand, within BBR_RS12335 at 520.985 kb on + strand at 521.009 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.019 kb on + strand at 521.026 kb on + strand at 521.027 kb on - strand at 521.027 kb on - strand at 521.027 kb on - strand at 521.027 kb on - strand at 521.027 kb on - strand at 521.034 kb on - strand at 521.034 kb on - strand at 521.240 kb on + strand, within BBR_RS12340 at 521.248 kb on - strand, within BBR_RS12340 at 521.281 kb on + strand at 521.294 kb on - strand at 521.339 kb on + strand at 521.387 kb on + strand at 521.388 kb on + strand at 521.396 kb on - strand at 521.398 kb on + strand at 521.398 kb on + strand at 521.416 kb on + strand at 521.423 kb on - strand at 521.477 kb on + strand at 521.477 kb on + strand at 521.485 kb on - strand at 521.506 kb on - strand at 521.513 kb on + strand at 521.513 kb on + strand at 521.513 kb on + strand at 521.519 kb on + strand, within BBR_RS12345 at 521.521 kb on - strand, within BBR_RS12345 at 521.565 kb on + strand, within BBR_RS12345 at 521.568 kb on - strand, within BBR_RS12345 at 521.576 kb on - strand, within BBR_RS12345 at 521.577 kb on + strand, within BBR_RS12345 at 521.577 kb on + strand, within BBR_RS12345 at 521.577 kb on + strand, within BBR_RS12345 at 521.595 kb on - strand, within BBR_RS12345 at 521.595 kb on - strand, within BBR_RS12345 at 521.669 kb on - strand, within BBR_RS12345 at 521.692 kb on + strand, within BBR_RS12345 at 521.700 kb on - strand, within BBR_RS12345 at 521.700 kb on - strand, within BBR_RS12345 at 521.701 kb on - strand, within BBR_RS12345 at 521.721 kb on - strand, within BBR_RS12345 at 521.723 kb on + strand, within BBR_RS12345 at 521.759 kb on + strand, within BBR_RS12345 at 521.769 kb on + strand, within BBR_RS12345 at 521.796 kb on - strand, within BBR_RS12345 at 521.824 kb on + strand, within BBR_RS12345 at 521.832 kb on - strand, within BBR_RS12345 at 521.832 kb on - strand, within BBR_RS12345 at 521.832 kb on - strand, within BBR_RS12345 at 521.857 kb on - strand, within BBR_RS12345 at 521.861 kb on - strand, within BBR_RS12345 at 521.870 kb on + strand, within BBR_RS12345 at 521.878 kb on + strand, within BBR_RS12345 at 521.878 kb on + strand, within BBR_RS12345 at 521.878 kb on - strand, within BBR_RS12345 at 521.878 kb on - strand, within BBR_RS12345 at 521.880 kb on - strand, within BBR_RS12345 at 521.884 kb on - strand, within BBR_RS12345 at 521.942 kb on + strand, within BBR_RS12345 at 521.942 kb on + strand, within BBR_RS12345 at 521.942 kb on - strand, within BBR_RS12345 at 521.942 kb on - strand, within BBR_RS12345 at 521.944 kb on + strand, within BBR_RS12345 at 521.950 kb on - strand, within BBR_RS12345 at 521.955 kb on - strand, within BBR_RS12345 at 521.955 kb on - strand, within BBR_RS12345 at 521.978 kb on - strand at 521.980 kb on - strand at 521.980 kb on - strand at 521.999 kb on + strand at 522.027 kb on + strand at 522.034 kb on + strand at 522.034 kb on + strand at 522.050 kb on + strand at 522.055 kb on + strand at 522.062 kb on - strand at 522.082 kb on + strand at 522.134 kb on - strand at 522.134 kb on - strand at 522.142 kb on - strand at 522.193 kb on - strand at 522.195 kb on + strand at 522.197 kb on + strand at 522.206 kb on + strand at 522.238 kb on - strand, within BBR_RS12350 at 522.241 kb on + strand, within BBR_RS12350 at 522.241 kb on + strand, within BBR_RS12350 at 522.264 kb on - strand, within BBR_RS12350 at 522.286 kb on + strand, within BBR_RS12350 at 522.289 kb on - strand, within BBR_RS12350
Per-strain Table
Position Strand Gene LocusTag Fraction MRS + kanamycin Time48hr remove 520,021 + BBR_RS12330 0.81 -0.2 520,032 - BBR_RS12330 0.81 +0.2 520,032 - BBR_RS12330 0.81 -1.5 520,032 - BBR_RS12330 0.81 +1.8 520,032 - BBR_RS12330 0.81 -0.5 520,032 - BBR_RS12330 0.81 -0.2 520,044 + BBR_RS12330 0.82 -0.2 520,047 - BBR_RS12330 0.82 -2.2 520,052 - BBR_RS12330 0.82 -1.2 520,055 + BBR_RS12330 0.82 +0.2 520,055 + BBR_RS12330 0.82 +1.2 520,063 + BBR_RS12330 0.83 -0.8 520,090 + BBR_RS12330 0.84 +0.8 520,114 - BBR_RS12330 0.85 +1.7 520,123 - BBR_RS12330 0.85 -1.6 520,127 + BBR_RS12330 0.86 +0.2 520,140 - BBR_RS12330 0.86 +1.2 520,144 + BBR_RS12330 0.86 -1.5 520,144 + BBR_RS12330 0.86 -0.4 520,144 + BBR_RS12330 0.86 +1.2 520,144 + BBR_RS12330 0.86 -0.8 520,144 + BBR_RS12330 0.86 -1.2 520,144 + BBR_RS12330 0.86 -2.8 520,144 + BBR_RS12330 0.86 +1.8 520,144 + BBR_RS12330 0.86 -0.8 520,145 + BBR_RS12330 0.86 -0.3 520,145 + BBR_RS12330 0.86 -0.3 520,146 - BBR_RS12330 0.86 -1.8 520,152 + BBR_RS12330 0.87 +0.1 520,152 - BBR_RS12330 0.87 +1.3 520,152 - BBR_RS12330 0.87 -1.9 520,152 - BBR_RS12330 0.87 -2.3 520,152 - BBR_RS12330 0.87 -0.8 520,152 - BBR_RS12330 0.87 +1.4 520,152 - BBR_RS12330 0.87 +2.2 520,152 - BBR_RS12330 0.87 +3.1 520,152 - BBR_RS12330 0.87 -2.2 520,152 - BBR_RS12330 0.87 +2.2 520,160 + BBR_RS12330 0.87 +2.6 520,212 + BBR_RS12330 0.89 -1.3 520,220 - BBR_RS12330 0.90 -2.9 520,220 - BBR_RS12330 0.90 -1.3 520,246 + -2.9 520,252 - +0.2 520,254 - +1.8 520,326 + +2.2 520,326 + -2.3 520,326 + -0.8 520,326 + -0.8 520,326 + -1.8 520,334 - +0.2 520,334 - -2.8 520,334 - -2.9 520,334 - +1.2 520,334 - +0.2 520,334 - +0.8 520,385 - +1.2 520,416 + +0.2 520,424 - -0.5 520,433 - -1.6 520,457 + +1.8 520,457 + -3.1 520,457 + +1.4 520,465 - +0.2 520,497 - -0.3 520,500 + +1.4 520,530 - -1.3 520,530 - +1.8 520,531 - -3.0 520,550 - +0.6 520,557 + -1.1 520,560 - -0.8 520,582 + +1.0 520,582 + -1.3 520,582 + -0.2 520,582 + -0.2 520,590 - +1.7 520,607 - -3.1 520,609 + +0.2 520,653 + BBR_RS12335 0.16 +1.7 520,667 + BBR_RS12335 0.19 -0.8 520,675 - BBR_RS12335 0.21 +0.8 520,709 + BBR_RS12335 0.29 -0.2 520,801 + BBR_RS12335 0.51 +0.4 520,809 - BBR_RS12335 0.53 -1.1 520,814 + BBR_RS12335 0.54 +0.2 520,830 - BBR_RS12335 0.58 -1.3 520,830 - BBR_RS12335 0.58 +0.4 520,847 - BBR_RS12335 0.62 -2.9 520,858 - BBR_RS12335 0.65 -0.3 520,879 - BBR_RS12335 0.70 -0.1 520,891 + BBR_RS12335 0.72 -1.8 520,896 + BBR_RS12335 0.74 -0.4 520,985 + -2.3 521,009 + -0.4 521,019 + -0.3 521,019 + -1.3 521,019 + -1.3 521,019 + +0.2 521,019 + +0.2 521,019 + +0.2 521,019 + +0.9 521,019 + -1.3 521,019 + -0.2 521,019 + +0.2 521,019 + +0.2 521,026 + +0.2 521,027 - +0.2 521,027 - -0.9 521,027 - +1.2 521,027 - +0.5 521,027 - -2.1 521,034 - +0.3 521,034 - -2.2 521,240 + BBR_RS12340 0.80 -0.5 521,248 - BBR_RS12340 0.83 -2.1 521,281 + -0.3 521,294 - -1.3 521,339 + -2.3 521,387 + +1.8 521,388 + +2.6 521,396 - +1.4 521,398 + +1.2 521,398 + -0.6 521,416 + -0.8 521,423 - -1.1 521,477 + +0.2 521,477 + +0.2 521,485 - +2.2 521,506 - -0.8 521,513 + -2.4 521,513 + +1.5 521,513 + -0.7 521,519 + BBR_RS12345 0.10 +1.8 521,521 - BBR_RS12345 0.10 +0.2 521,565 + BBR_RS12345 0.18 -1.0 521,568 - BBR_RS12345 0.19 -0.9 521,576 - BBR_RS12345 0.20 +0.7 521,577 + BBR_RS12345 0.20 +0.0 521,577 + BBR_RS12345 0.20 -0.3 521,577 + BBR_RS12345 0.20 +0.2 521,595 - BBR_RS12345 0.24 -0.6 521,595 - BBR_RS12345 0.24 +1.6 521,669 - BBR_RS12345 0.37 +1.5 521,692 + BBR_RS12345 0.41 +0.2 521,700 - BBR_RS12345 0.42 +1.8 521,700 - BBR_RS12345 0.42 +0.2 521,701 - BBR_RS12345 0.43 -0.2 521,721 - BBR_RS12345 0.46 +0.5 521,723 + BBR_RS12345 0.47 -1.7 521,759 + BBR_RS12345 0.53 -1.1 521,769 + BBR_RS12345 0.55 -0.9 521,796 - BBR_RS12345 0.60 -0.9 521,824 + BBR_RS12345 0.65 +2.6 521,832 - BBR_RS12345 0.66 -0.5 521,832 - BBR_RS12345 0.66 +2.3 521,832 - BBR_RS12345 0.66 -1.3 521,857 - BBR_RS12345 0.71 -0.2 521,861 - BBR_RS12345 0.71 -2.9 521,870 + BBR_RS12345 0.73 +0.2 521,878 + BBR_RS12345 0.74 -1.6 521,878 + BBR_RS12345 0.74 -0.0 521,878 - BBR_RS12345 0.74 -1.9 521,878 - BBR_RS12345 0.74 -1.8 521,880 - BBR_RS12345 0.75 +0.0 521,884 - BBR_RS12345 0.75 -1.3 521,942 + BBR_RS12345 0.86 -0.3 521,942 + BBR_RS12345 0.86 -1.3 521,942 - BBR_RS12345 0.86 -0.8 521,942 - BBR_RS12345 0.86 +3.4 521,944 + BBR_RS12345 0.86 -3.3 521,950 - BBR_RS12345 0.87 -0.1 521,955 - BBR_RS12345 0.88 -0.2 521,955 - BBR_RS12345 0.88 -2.2 521,978 - +1.2 521,980 - -0.4 521,980 - -2.5 521,999 + +0.2 522,027 + +0.2 522,034 + +0.2 522,034 + -0.8 522,050 + +2.4 522,055 + -1.0 522,062 - +1.0 522,082 + -0.9 522,134 - -1.9 522,134 - -1.3 522,142 - +0.4 522,193 - +1.2 522,195 + +0.2 522,197 + +0.2 522,206 + +1.4 522,238 - BBR_RS12350 0.10 +2.1 522,241 + BBR_RS12350 0.10 -1.0 522,241 + BBR_RS12350 0.10 -1.1 522,264 - BBR_RS12350 0.12 -2.1 522,286 + BBR_RS12350 0.13 +0.2 522,289 - BBR_RS12350 0.13 +0.6
Or see this region's nucleotide sequence