Experiment: MRS Time48hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13560 and BBR_RS13565 are separated by 7 nucleotides BBR_RS13565 and BBR_RS20785 are separated by 299 nucleotides BBR_RS20785 and BBR_RS13575 are separated by 39 nucleotides BBR_RS13575 and BBR_RS13580 are separated by 9 nucleotides
BBR_RS13560: BBR_RS13560 - MATE family efflux transporter, at 814,983 to 816,341
_RS13560
BBR_RS13565: BBR_RS13565 - DNA-(apurinic or apyrimidinic site) lyase endonuclease III base excision repair, at 816,349 to 817,041
_RS13565
BBR_RS20785: BBR_RS20785 - PadR family transcriptional regulator, at 817,341 to 817,487
_RS20785
BBR_RS13575: BBR_RS13575 - sugar O-acetyltransferase, at 817,527 to 818,201
_RS13575
BBR_RS13580: BBR_RS13580 - nodulation protein L, at 818,211 to 818,474
_RS13580
Position (kb) 817
818 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 816.381 kb on + strand at 816.420 kb on + strand, within BBR_RS13565 at 816.420 kb on + strand, within BBR_RS13565 at 816.447 kb on - strand, within BBR_RS13565 at 816.448 kb on - strand, within BBR_RS13565 at 816.448 kb on - strand, within BBR_RS13565 at 816.462 kb on - strand, within BBR_RS13565 at 816.462 kb on - strand, within BBR_RS13565 at 816.525 kb on - strand, within BBR_RS13565 at 816.534 kb on - strand, within BBR_RS13565 at 816.549 kb on + strand, within BBR_RS13565 at 816.567 kb on + strand, within BBR_RS13565 at 816.569 kb on - strand, within BBR_RS13565 at 816.607 kb on - strand, within BBR_RS13565 at 816.632 kb on + strand, within BBR_RS13565 at 816.640 kb on - strand, within BBR_RS13565 at 816.663 kb on + strand, within BBR_RS13565 at 816.666 kb on - strand, within BBR_RS13565 at 816.685 kb on - strand, within BBR_RS13565 at 816.687 kb on + strand, within BBR_RS13565 at 816.704 kb on - strand, within BBR_RS13565 at 816.787 kb on + strand, within BBR_RS13565 at 816.855 kb on - strand, within BBR_RS13565 at 816.894 kb on + strand, within BBR_RS13565 at 817.078 kb on + strand at 817.226 kb on + strand at 817.226 kb on + strand at 817.226 kb on + strand at 817.226 kb on + strand at 817.313 kb on - strand at 817.371 kb on - strand, within BBR_RS20785 at 817.371 kb on - strand, within BBR_RS20785 at 817.603 kb on + strand, within BBR_RS13575 at 817.700 kb on - strand, within BBR_RS13575 at 817.755 kb on + strand, within BBR_RS13575 at 817.755 kb on + strand, within BBR_RS13575 at 817.763 kb on - strand, within BBR_RS13575 at 817.793 kb on + strand, within BBR_RS13575 at 817.810 kb on - strand, within BBR_RS13575 at 817.852 kb on - strand, within BBR_RS13575 at 817.852 kb on - strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.927 kb on + strand, within BBR_RS13575 at 817.968 kb on + strand, within BBR_RS13575 at 817.998 kb on - strand, within BBR_RS13575 at 818.028 kb on + strand, within BBR_RS13575 at 818.043 kb on + strand, within BBR_RS13575 at 818.116 kb on - strand, within BBR_RS13575 at 818.116 kb on - strand, within BBR_RS13575 at 818.163 kb on + strand at 818.191 kb on + strand at 818.191 kb on + strand at 818.201 kb on - strand at 818.299 kb on - strand, within BBR_RS13580 at 818.317 kb on + strand, within BBR_RS13580 at 818.317 kb on + strand, within BBR_RS13580 at 818.319 kb on + strand, within BBR_RS13580 at 818.336 kb on - strand, within BBR_RS13580 at 818.401 kb on - strand, within BBR_RS13580 at 818.407 kb on + strand, within BBR_RS13580 at 818.424 kb on + strand, within BBR_RS13580 at 818.429 kb on + strand, within BBR_RS13580 at 818.443 kb on + strand, within BBR_RS13580 at 818.447 kb on + strand, within BBR_RS13580 at 818.451 kb on - strand at 818.451 kb on - strand at 818.475 kb on - strand at 818.475 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MRS Time48hr remove 816,381 + +1.4 816,420 + BBR_RS13565 0.10 +1.2 816,420 + BBR_RS13565 0.10 -0.4 816,447 - BBR_RS13565 0.14 +1.4 816,448 - BBR_RS13565 0.14 +0.1 816,448 - BBR_RS13565 0.14 -1.2 816,462 - BBR_RS13565 0.16 -0.8 816,462 - BBR_RS13565 0.16 +3.7 816,525 - BBR_RS13565 0.25 -0.6 816,534 - BBR_RS13565 0.27 -1.5 816,549 + BBR_RS13565 0.29 +2.4 816,567 + BBR_RS13565 0.31 +0.4 816,569 - BBR_RS13565 0.32 -1.5 816,607 - BBR_RS13565 0.37 -3.2 816,632 + BBR_RS13565 0.41 +3.9 816,640 - BBR_RS13565 0.42 -1.0 816,663 + BBR_RS13565 0.45 -0.8 816,666 - BBR_RS13565 0.46 +0.7 816,685 - BBR_RS13565 0.48 -0.2 816,687 + BBR_RS13565 0.49 -0.8 816,704 - BBR_RS13565 0.51 +0.4 816,787 + BBR_RS13565 0.63 +2.9 816,855 - BBR_RS13565 0.73 +1.4 816,894 + BBR_RS13565 0.79 -0.8 817,078 + +0.4 817,226 + +1.4 817,226 + +1.4 817,226 + +0.2 817,226 + -0.2 817,313 - -1.5 817,371 - BBR_RS20785 0.20 +1.4 817,371 - BBR_RS20785 0.20 +1.5 817,603 + BBR_RS13575 0.11 +2.4 817,700 - BBR_RS13575 0.26 +0.4 817,755 + BBR_RS13575 0.34 +1.4 817,755 + BBR_RS13575 0.34 -1.2 817,763 - BBR_RS13575 0.35 -2.6 817,793 + BBR_RS13575 0.39 -1.2 817,810 - BBR_RS13575 0.42 -1.2 817,852 - BBR_RS13575 0.48 -1.6 817,852 - BBR_RS13575 0.48 -0.0 817,927 + BBR_RS13575 0.59 -1.6 817,927 + BBR_RS13575 0.59 -1.5 817,927 + BBR_RS13575 0.59 -0.5 817,927 + BBR_RS13575 0.59 -1.2 817,927 + BBR_RS13575 0.59 -1.0 817,927 + BBR_RS13575 0.59 -0.5 817,927 + BBR_RS13575 0.59 -2.3 817,968 + BBR_RS13575 0.65 -0.8 817,998 - BBR_RS13575 0.70 -0.6 818,028 + BBR_RS13575 0.74 +0.3 818,043 + BBR_RS13575 0.76 +1.4 818,116 - BBR_RS13575 0.87 +0.3 818,116 - BBR_RS13575 0.87 +2.9 818,163 + +4.2 818,191 + +0.3 818,191 + +1.3 818,201 - +0.1 818,299 - BBR_RS13580 0.33 +0.0 818,317 + BBR_RS13580 0.40 -1.5 818,317 + BBR_RS13580 0.40 +1.3 818,319 + BBR_RS13580 0.41 -0.7 818,336 - BBR_RS13580 0.47 +2.3 818,401 - BBR_RS13580 0.72 +0.3 818,407 + BBR_RS13580 0.74 +2.9 818,424 + BBR_RS13580 0.81 -2.1 818,429 + BBR_RS13580 0.83 -0.2 818,443 + BBR_RS13580 0.88 +0.3 818,447 + BBR_RS13580 0.89 +2.3 818,451 - -1.7 818,451 - +1.3 818,475 - +1.3 818,475 - -1.2
Or see this region's nucleotide sequence