Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_27520 and AO356_27525 are separated by 106 nucleotides AO356_27525 and AO356_27530 are separated by 200 nucleotides
AO356_27520: AO356_27520 - betaine-aldehyde dehydrogenase, at 6,272,395 to 6,273,849
_27520
AO356_27525: AO356_27525 - cytochrome B, at 6,273,956 to 6,274,519
_27525
AO356_27530: AO356_27530 - hypothetical protein, at 6,274,720 to 6,278,454
_27530
Position (kb)
6273
6274
6275 Strain fitness (log2 ratio)
-1
0
1 at 6273.347 kb on + strand, within AO356_27520 at 6273.348 kb on - strand, within AO356_27520 at 6273.629 kb on - strand, within AO356_27520 at 6273.808 kb on + strand at 6274.097 kb on + strand, within AO356_27525 at 6274.098 kb on - strand, within AO356_27525 at 6274.098 kb on - strand, within AO356_27525 at 6274.106 kb on + strand, within AO356_27525 at 6274.150 kb on - strand, within AO356_27525 at 6274.221 kb on - strand, within AO356_27525 at 6274.260 kb on - strand, within AO356_27525 at 6274.418 kb on + strand, within AO356_27525 at 6274.475 kb on + strand at 6274.476 kb on - strand at 6274.534 kb on - strand at 6274.608 kb on - strand at 6274.679 kb on + strand at 6274.719 kb on - strand at 6275.098 kb on - strand, within AO356_27530 at 6275.278 kb on + strand, within AO356_27530 at 6275.279 kb on - strand, within AO356_27530 at 6275.279 kb on - strand, within AO356_27530 at 6275.282 kb on + strand, within AO356_27530 at 6275.282 kb on + strand, within AO356_27530 at 6275.282 kb on + strand, within AO356_27530 at 6275.282 kb on + strand, within AO356_27530 at 6275.283 kb on - strand, within AO356_27530 at 6275.283 kb on - strand, within AO356_27530 at 6275.308 kb on - strand, within AO356_27530 at 6275.308 kb on - strand, within AO356_27530
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 6,273,347 + AO356_27520 0.65 -0.1 6,273,348 - AO356_27520 0.65 +0.4 6,273,629 - AO356_27520 0.85 +1.3 6,273,808 + +0.7 6,274,097 + AO356_27525 0.25 -0.6 6,274,098 - AO356_27525 0.25 +0.6 6,274,098 - AO356_27525 0.25 +0.1 6,274,106 + AO356_27525 0.27 -1.0 6,274,150 - AO356_27525 0.34 +0.3 6,274,221 - AO356_27525 0.47 -0.8 6,274,260 - AO356_27525 0.54 +0.2 6,274,418 + AO356_27525 0.82 +0.1 6,274,475 + +0.4 6,274,476 - +0.9 6,274,534 - +0.9 6,274,608 - -1.1 6,274,679 + +1.1 6,274,719 - +0.2 6,275,098 - AO356_27530 0.10 +1.6 6,275,278 + AO356_27530 0.15 -0.3 6,275,279 - AO356_27530 0.15 +0.3 6,275,279 - AO356_27530 0.15 +0.8 6,275,282 + AO356_27530 0.15 +0.1 6,275,282 + AO356_27530 0.15 -0.0 6,275,282 + AO356_27530 0.15 +0.5 6,275,282 + AO356_27530 0.15 -0.7 6,275,283 - AO356_27530 0.15 -0.1 6,275,283 - AO356_27530 0.15 +0.7 6,275,308 - AO356_27530 0.16 +0.4 6,275,308 - AO356_27530 0.16 +0.6
Or see this region's nucleotide sequence