Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_22910 and AO356_22915 are separated by 56 nucleotides AO356_22915 and AO356_22920 are separated by 41 nucleotides AO356_22920 and AO356_22925 are separated by 570 nucleotides
AO356_22910: AO356_22910 - histidine kinase, at 5,115,931 to 5,117,232
_22910
AO356_22915: AO356_22915 - hypothetical protein, at 5,117,289 to 5,117,930
_22915
AO356_22920: AO356_22920 - nicotinamidase, at 5,117,972 to 5,118,625
_22920
AO356_22925: AO356_22925 - histidine kinase, at 5,119,196 to 5,121,274
_22925
Position (kb) 5117
5118
5119 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5117.312 kb on + strand at 5117.373 kb on + strand, within AO356_22915 at 5117.450 kb on - strand, within AO356_22915 at 5117.464 kb on + strand, within AO356_22915 at 5117.464 kb on + strand, within AO356_22915 at 5117.465 kb on - strand, within AO356_22915 at 5117.465 kb on - strand, within AO356_22915 at 5117.465 kb on - strand, within AO356_22915 at 5117.524 kb on + strand, within AO356_22915 at 5117.524 kb on + strand, within AO356_22915 at 5117.524 kb on + strand, within AO356_22915 at 5117.524 kb on + strand, within AO356_22915 at 5117.525 kb on - strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.527 kb on + strand, within AO356_22915 at 5117.528 kb on - strand, within AO356_22915 at 5117.605 kb on + strand, within AO356_22915 at 5117.605 kb on + strand, within AO356_22915 at 5117.605 kb on + strand, within AO356_22915 at 5117.606 kb on - strand, within AO356_22915 at 5117.865 kb on - strand, within AO356_22915 at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.960 kb on + strand at 5117.961 kb on - strand at 5117.961 kb on - strand at 5117.961 kb on - strand at 5117.961 kb on - strand at 5117.961 kb on - strand at 5117.963 kb on + strand at 5117.964 kb on - strand at 5118.174 kb on + strand, within AO356_22920 at 5118.255 kb on + strand, within AO356_22920 at 5118.256 kb on - strand, within AO356_22920 at 5118.256 kb on - strand, within AO356_22920 at 5118.351 kb on - strand, within AO356_22920 at 5118.351 kb on - strand, within AO356_22920 at 5118.471 kb on - strand, within AO356_22920 at 5118.471 kb on - strand, within AO356_22920 at 5118.572 kb on + strand at 5118.572 kb on + strand at 5118.572 kb on + strand at 5118.573 kb on - strand at 5118.660 kb on + strand at 5118.661 kb on - strand at 5118.745 kb on + strand at 5118.745 kb on + strand at 5118.882 kb on - strand at 5118.882 kb on - strand at 5119.076 kb on + strand at 5119.182 kb on + strand at 5119.183 kb on - strand at 5119.211 kb on + strand at 5119.262 kb on + strand at 5119.262 kb on + strand at 5119.262 kb on + strand at 5119.263 kb on - strand at 5119.289 kb on - strand at 5119.416 kb on - strand, within AO356_22925 at 5119.562 kb on + strand, within AO356_22925 at 5119.562 kb on + strand, within AO356_22925 at 5119.562 kb on + strand, within AO356_22925 at 5119.563 kb on - strand, within AO356_22925 at 5119.563 kb on - strand, within AO356_22925 at 5119.563 kb on - strand, within AO356_22925
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 5,117,312 + +0.2 5,117,373 + AO356_22915 0.13 +0.8 5,117,450 - AO356_22915 0.25 +0.3 5,117,464 + AO356_22915 0.27 -2.5 5,117,464 + AO356_22915 0.27 -0.6 5,117,465 - AO356_22915 0.27 +0.1 5,117,465 - AO356_22915 0.27 +0.4 5,117,465 - AO356_22915 0.27 -0.5 5,117,524 + AO356_22915 0.37 -0.2 5,117,524 + AO356_22915 0.37 +1.1 5,117,524 + AO356_22915 0.37 -0.5 5,117,524 + AO356_22915 0.37 +0.5 5,117,525 - AO356_22915 0.37 +0.9 5,117,527 + AO356_22915 0.37 +0.0 5,117,527 + AO356_22915 0.37 -0.4 5,117,527 + AO356_22915 0.37 -0.7 5,117,527 + AO356_22915 0.37 -0.2 5,117,527 + AO356_22915 0.37 -0.1 5,117,528 - AO356_22915 0.37 -0.4 5,117,605 + AO356_22915 0.49 +0.5 5,117,605 + AO356_22915 0.49 -0.5 5,117,605 + AO356_22915 0.49 +0.4 5,117,606 - AO356_22915 0.49 -0.7 5,117,865 - AO356_22915 0.90 +0.7 5,117,960 + +0.6 5,117,960 + +0.7 5,117,960 + +1.7 5,117,960 + -0.6 5,117,960 + +0.5 5,117,960 + -0.0 5,117,961 - -0.2 5,117,961 - +0.3 5,117,961 - +0.6 5,117,961 - +0.3 5,117,961 - -0.1 5,117,963 + +0.1 5,117,964 - +1.0 5,118,174 + AO356_22920 0.31 -0.5 5,118,255 + AO356_22920 0.43 -0.0 5,118,256 - AO356_22920 0.43 +0.0 5,118,256 - AO356_22920 0.43 +0.8 5,118,351 - AO356_22920 0.58 -0.7 5,118,351 - AO356_22920 0.58 -2.1 5,118,471 - AO356_22920 0.76 -0.4 5,118,471 - AO356_22920 0.76 -0.5 5,118,572 + -0.2 5,118,572 + +0.4 5,118,572 + +0.4 5,118,573 - +1.2 5,118,660 + +0.5 5,118,661 - +0.5 5,118,745 + -0.8 5,118,745 + +0.1 5,118,882 - -0.8 5,118,882 - -0.6 5,119,076 + -0.5 5,119,182 + -0.9 5,119,183 - -0.0 5,119,211 + +1.3 5,119,262 + +1.9 5,119,262 + +0.2 5,119,262 + -0.0 5,119,263 - +1.1 5,119,289 - +0.8 5,119,416 - AO356_22925 0.11 -0.8 5,119,562 + AO356_22925 0.18 -0.1 5,119,562 + AO356_22925 0.18 -0.0 5,119,562 + AO356_22925 0.18 +0.7 5,119,563 - AO356_22925 0.18 +1.3 5,119,563 - AO356_22925 0.18 +0.6 5,119,563 - AO356_22925 0.18 +0.1
Or see this region's nucleotide sequence