Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_19845 and AO356_19850 are separated by 290 nucleotides AO356_19850 and AO356_19855 are separated by 120 nucleotides
AO356_19845: AO356_19845 - transcriptional regulator, at 4,449,043 to 4,449,801
_19845
AO356_19850: AO356_19850 - orotidine 5'-phosphate decarboxylase, at 4,450,092 to 4,450,790
_19850
AO356_19855: AO356_19855 - NADP-dependent oxidoreductase, at 4,450,911 to 4,451,915
_19855
Position (kb)
4450
4451 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4449.146 kb on - strand, within AO356_19845 at 4449.446 kb on + strand, within AO356_19845 at 4449.446 kb on + strand, within AO356_19845 at 4449.446 kb on + strand, within AO356_19845 at 4449.446 kb on + strand, within AO356_19845 at 4449.446 kb on + strand, within AO356_19845 at 4449.446 kb on + strand, within AO356_19845 at 4449.447 kb on - strand, within AO356_19845 at 4449.447 kb on - strand, within AO356_19845 at 4449.447 kb on - strand, within AO356_19845 at 4449.447 kb on - strand, within AO356_19845 at 4449.447 kb on - strand, within AO356_19845 at 4449.447 kb on - strand, within AO356_19845 at 4449.661 kb on + strand, within AO356_19845 at 4449.821 kb on + strand at 4449.822 kb on - strand at 4449.866 kb on + strand at 4449.866 kb on + strand at 4449.867 kb on - strand at 4449.941 kb on + strand at 4449.941 kb on + strand at 4449.941 kb on + strand at 4449.941 kb on + strand at 4449.941 kb on + strand at 4449.941 kb on + strand at 4449.941 kb on + strand at 4449.942 kb on - strand at 4449.942 kb on - strand at 4449.942 kb on - strand at 4449.958 kb on + strand at 4449.958 kb on + strand at 4449.958 kb on + strand at 4449.959 kb on - strand at 4450.016 kb on - strand at 4450.052 kb on + strand at 4450.053 kb on - strand at 4450.343 kb on - strand, within AO356_19850 at 4450.839 kb on + strand at 4450.839 kb on + strand at 4450.839 kb on + strand at 4450.840 kb on - strand at 4450.840 kb on - strand at 4450.840 kb on - strand at 4450.923 kb on + strand at 4450.923 kb on + strand at 4450.924 kb on - strand at 4451.074 kb on + strand, within AO356_19855 at 4451.075 kb on - strand, within AO356_19855 at 4451.128 kb on + strand, within AO356_19855 at 4451.128 kb on + strand, within AO356_19855 at 4451.129 kb on - strand, within AO356_19855 at 4451.129 kb on - strand, within AO356_19855 at 4451.165 kb on - strand, within AO356_19855 at 4451.165 kb on - strand, within AO356_19855 at 4451.257 kb on + strand, within AO356_19855 at 4451.257 kb on + strand, within AO356_19855 at 4451.317 kb on + strand, within AO356_19855 at 4451.317 kb on + strand, within AO356_19855 at 4451.545 kb on + strand, within AO356_19855 at 4451.545 kb on + strand, within AO356_19855 at 4451.546 kb on - strand, within AO356_19855 at 4451.546 kb on - strand, within AO356_19855 at 4451.546 kb on - strand, within AO356_19855 at 4451.546 kb on - strand, within AO356_19855 at 4451.581 kb on + strand, within AO356_19855 at 4451.582 kb on - strand, within AO356_19855 at 4451.609 kb on - strand, within AO356_19855 at 4451.609 kb on - strand, within AO356_19855 at 4451.638 kb on + strand, within AO356_19855 at 4451.731 kb on + strand, within AO356_19855 at 4451.732 kb on - strand, within AO356_19855 at 4451.782 kb on + strand, within AO356_19855 at 4451.782 kb on + strand, within AO356_19855 at 4451.783 kb on - strand, within AO356_19855 at 4451.783 kb on - strand, within AO356_19855 at 4451.783 kb on - strand, within AO356_19855
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 4,449,146 - AO356_19845 0.14 +1.0 4,449,446 + AO356_19845 0.53 -1.0 4,449,446 + AO356_19845 0.53 -0.3 4,449,446 + AO356_19845 0.53 +0.5 4,449,446 + AO356_19845 0.53 -0.1 4,449,446 + AO356_19845 0.53 -0.2 4,449,446 + AO356_19845 0.53 +0.0 4,449,447 - AO356_19845 0.53 +0.0 4,449,447 - AO356_19845 0.53 -0.1 4,449,447 - AO356_19845 0.53 -1.3 4,449,447 - AO356_19845 0.53 +0.4 4,449,447 - AO356_19845 0.53 -0.0 4,449,447 - AO356_19845 0.53 +0.4 4,449,661 + AO356_19845 0.81 +0.0 4,449,821 + +0.9 4,449,822 - +1.1 4,449,866 + +0.6 4,449,866 + -0.1 4,449,867 - +0.8 4,449,941 + +0.0 4,449,941 + +0.7 4,449,941 + -0.0 4,449,941 + -0.7 4,449,941 + -0.1 4,449,941 + +0.1 4,449,941 + +0.3 4,449,942 - +0.4 4,449,942 - +0.7 4,449,942 - +0.7 4,449,958 + +0.7 4,449,958 + -0.7 4,449,958 + -0.1 4,449,959 - -0.4 4,450,016 - -1.1 4,450,052 + -0.3 4,450,053 - +0.9 4,450,343 - AO356_19850 0.36 -0.3 4,450,839 + +0.1 4,450,839 + -0.3 4,450,839 + +0.4 4,450,840 - -0.6 4,450,840 - +0.4 4,450,840 - +0.5 4,450,923 + +0.0 4,450,923 + +0.4 4,450,924 - -0.3 4,451,074 + AO356_19855 0.16 -0.3 4,451,075 - AO356_19855 0.16 -0.1 4,451,128 + AO356_19855 0.22 -0.6 4,451,128 + AO356_19855 0.22 +0.7 4,451,129 - AO356_19855 0.22 +0.1 4,451,129 - AO356_19855 0.22 -1.0 4,451,165 - AO356_19855 0.25 +0.7 4,451,165 - AO356_19855 0.25 +0.4 4,451,257 + AO356_19855 0.34 -0.6 4,451,257 + AO356_19855 0.34 -0.1 4,451,317 + AO356_19855 0.40 +0.4 4,451,317 + AO356_19855 0.40 -0.7 4,451,545 + AO356_19855 0.63 +1.4 4,451,545 + AO356_19855 0.63 +0.5 4,451,546 - AO356_19855 0.63 +0.2 4,451,546 - AO356_19855 0.63 -0.3 4,451,546 - AO356_19855 0.63 +2.5 4,451,546 - AO356_19855 0.63 +1.7 4,451,581 + AO356_19855 0.67 +0.3 4,451,582 - AO356_19855 0.67 +0.2 4,451,609 - AO356_19855 0.69 +0.6 4,451,609 - AO356_19855 0.69 -0.4 4,451,638 + AO356_19855 0.72 -0.1 4,451,731 + AO356_19855 0.82 +0.6 4,451,732 - AO356_19855 0.82 -1.8 4,451,782 + AO356_19855 0.87 +0.2 4,451,782 + AO356_19855 0.87 -0.0 4,451,783 - AO356_19855 0.87 +0.0 4,451,783 - AO356_19855 0.87 +0.4 4,451,783 - AO356_19855 0.87 -0.5
Or see this region's nucleotide sequence