Strain Fitness in Pseudomonas fluorescens FW300-N2C3 around AO356_15365

Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO356_15355 and AO356_15360 are separated by 72 nucleotidesAO356_15360 and AO356_15365 are separated by 227 nucleotidesAO356_15365 and AO356_15370 are separated by 39 nucleotidesAO356_15370 and AO356_15375 are separated by 90 nucleotides AO356_15355: AO356_15355 - murein transglycosylase, at 3,477,427 to 3,478,749 _15355 AO356_15360: AO356_15360 - lipoyl synthase, at 3,478,822 to 3,478,986 _15360 AO356_15365: AO356_15365 - DNA polymerase III subunit delta, at 3,479,214 to 3,480,251 _15365 AO356_15370: AO356_15370 - hypothetical protein, at 3,480,291 to 3,480,896 _15370 AO356_15375: AO356_15375 - leucine--tRNA ligase, at 3,480,987 to 3,483,593 _15375 Position (kb) 3479 3480 3481Strain fitness (log2 ratio) -2 -1 0 1at 3478.301 kb on + strand, within AO356_15355at 3478.394 kb on + strand, within AO356_15355at 3478.394 kb on + strand, within AO356_15355at 3478.394 kb on + strand, within AO356_15355at 3478.395 kb on - strand, within AO356_15355at 3478.660 kb on - strandat 3478.765 kb on + strandat 3478.765 kb on + strandat 3478.766 kb on - strandat 3478.785 kb on + strandat 3478.785 kb on + strandat 3478.785 kb on + strandat 3478.786 kb on - strandat 3478.786 kb on - strandat 3478.786 kb on - strandat 3478.804 kb on - strandat 3478.880 kb on + strand, within AO356_15360at 3479.215 kb on + strandat 3480.252 kb on - strandat 3480.252 kb on - strandat 3480.252 kb on - strandat 3480.293 kb on - strandat 3480.293 kb on - strandat 3480.919 kb on - strandat 3480.951 kb on - strandat 3480.951 kb on - strandat 3480.951 kb on - strandat 3480.951 kb on - strandat 3480.989 kb on - strandat 3480.989 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
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3,478,301 + AO356_15355 0.66 +0.4
3,478,394 + AO356_15355 0.73 +1.5
3,478,394 + AO356_15355 0.73 -0.0
3,478,394 + AO356_15355 0.73 -0.2
3,478,395 - AO356_15355 0.73 +0.3
3,478,660 - -1.5
3,478,765 + -0.5
3,478,765 + -0.0
3,478,766 - +0.1
3,478,785 + -0.0
3,478,785 + -0.0
3,478,785 + +1.0
3,478,786 - +0.7
3,478,786 - +0.5
3,478,786 - +1.0
3,478,804 - +0.4
3,478,880 + AO356_15360 0.35 -2.6
3,479,215 + +1.7
3,480,252 - -0.8
3,480,252 - -1.5
3,480,252 - -0.6
3,480,293 - -0.5
3,480,293 - -0.0
3,480,919 - -0.0
3,480,951 - +0.9
3,480,951 - +1.5
3,480,951 - -1.0
3,480,951 - -1.0
3,480,989 - -0.0
3,480,989 - -0.0

Or see this region's nucleotide sequence