Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_03795 and AO356_03800 are separated by 88 nucleotides AO356_03800 and AO356_03805 are separated by 31 nucleotides AO356_03805 and AO356_03810 are separated by 5 nucleotides AO356_03810 and AO356_03815 are separated by 102 nucleotides AO356_03815 and AO356_03820 are separated by 6 nucleotides
AO356_03795: AO356_03795 - flagellar hook-length control protein, at 840,711 to 842,084
_03795
AO356_03800: AO356_03800 - histidine kinase, at 842,173 to 842,517
_03800
AO356_03805: AO356_03805 - chemotaxis protein CheY, at 842,549 to 844,255
_03805
AO356_03810: AO356_03810 - anti-anti-sigma factor, at 844,261 to 844,566
_03810
AO356_03815: AO356_03815 - flagellar biogenesis protein, at 844,669 to 845,118
_03815
AO356_03820: AO356_03820 - flagellar protein export ATPase FliI, at 845,125 to 846,483
_03820
Position (kb) 842
843
844
845 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 841.988 kb on + strand at 842.085 kb on - strand at 842.112 kb on + strand at 842.112 kb on + strand at 842.112 kb on + strand at 842.112 kb on + strand at 842.113 kb on - strand at 842.113 kb on - strand at 842.113 kb on - strand at 842.113 kb on - strand at 842.116 kb on + strand at 842.116 kb on + strand at 842.116 kb on + strand at 842.117 kb on - strand at 842.128 kb on + strand at 842.128 kb on + strand at 842.129 kb on - strand at 842.129 kb on - strand at 842.437 kb on - strand, within AO356_03800 at 842.437 kb on - strand, within AO356_03800 at 842.550 kb on + strand at 842.551 kb on - strand at 842.588 kb on - strand at 842.781 kb on + strand, within AO356_03805 at 842.782 kb on - strand, within AO356_03805 at 842.809 kb on + strand, within AO356_03805 at 842.809 kb on + strand, within AO356_03805 at 842.810 kb on - strand, within AO356_03805 at 842.810 kb on - strand, within AO356_03805 at 842.823 kb on + strand, within AO356_03805 at 842.823 kb on + strand, within AO356_03805 at 842.823 kb on + strand, within AO356_03805 at 842.823 kb on + strand, within AO356_03805 at 842.824 kb on - strand, within AO356_03805 at 842.824 kb on - strand, within AO356_03805 at 842.824 kb on - strand, within AO356_03805 at 842.824 kb on - strand, within AO356_03805 at 842.901 kb on + strand, within AO356_03805 at 842.901 kb on + strand, within AO356_03805 at 842.901 kb on + strand, within AO356_03805 at 842.901 kb on + strand, within AO356_03805 at 842.920 kb on + strand, within AO356_03805 at 842.920 kb on + strand, within AO356_03805 at 842.921 kb on - strand, within AO356_03805 at 842.922 kb on + strand, within AO356_03805 at 842.922 kb on + strand, within AO356_03805 at 842.923 kb on - strand, within AO356_03805 at 842.923 kb on - strand, within AO356_03805 at 842.980 kb on - strand, within AO356_03805 at 843.070 kb on - strand, within AO356_03805 at 843.070 kb on - strand, within AO356_03805 at 843.269 kb on + strand, within AO356_03805 at 843.269 kb on + strand, within AO356_03805 at 843.269 kb on + strand, within AO356_03805 at 843.270 kb on - strand, within AO356_03805 at 843.270 kb on - strand, within AO356_03805 at 843.272 kb on + strand, within AO356_03805 at 843.273 kb on - strand, within AO356_03805 at 843.273 kb on - strand, within AO356_03805 at 843.273 kb on - strand, within AO356_03805 at 843.392 kb on - strand, within AO356_03805 at 843.392 kb on - strand, within AO356_03805 at 843.392 kb on - strand, within AO356_03805 at 843.421 kb on - strand, within AO356_03805 at 843.586 kb on - strand, within AO356_03805 at 843.717 kb on + strand, within AO356_03805 at 843.717 kb on + strand, within AO356_03805 at 843.718 kb on - strand, within AO356_03805 at 843.718 kb on - strand, within AO356_03805 at 843.816 kb on + strand, within AO356_03805 at 843.816 kb on + strand, within AO356_03805 at 843.816 kb on + strand, within AO356_03805 at 843.816 kb on + strand, within AO356_03805 at 843.816 kb on + strand, within AO356_03805 at 843.817 kb on - strand, within AO356_03805 at 843.817 kb on - strand, within AO356_03805 at 843.817 kb on - strand, within AO356_03805 at 843.817 kb on - strand, within AO356_03805 at 843.817 kb on - strand, within AO356_03805 at 843.921 kb on + strand, within AO356_03805 at 843.921 kb on + strand, within AO356_03805 at 843.921 kb on + strand, within AO356_03805 at 843.921 kb on + strand, within AO356_03805 at 843.922 kb on - strand, within AO356_03805 at 843.922 kb on - strand, within AO356_03805 at 843.922 kb on - strand, within AO356_03805 at 843.922 kb on - strand, within AO356_03805 at 843.922 kb on - strand, within AO356_03805 at 844.116 kb on + strand at 844.116 kb on + strand at 844.117 kb on - strand at 844.117 kb on - strand at 844.117 kb on - strand at 844.409 kb on + strand, within AO356_03810 at 844.410 kb on - strand, within AO356_03810 at 844.466 kb on + strand, within AO356_03810 at 844.466 kb on + strand, within AO356_03810 at 844.466 kb on + strand, within AO356_03810 at 844.466 kb on + strand, within AO356_03810 at 844.466 kb on + strand, within AO356_03810 at 844.467 kb on - strand, within AO356_03810 at 844.467 kb on - strand, within AO356_03810 at 844.467 kb on - strand, within AO356_03810 at 844.467 kb on - strand, within AO356_03810 at 844.547 kb on - strand at 844.547 kb on - strand at 844.590 kb on + strand at 844.590 kb on + strand at 844.590 kb on + strand at 844.590 kb on + strand at 844.590 kb on + strand at 844.590 kb on + strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.591 kb on - strand at 844.596 kb on + strand at 844.596 kb on + strand at 844.597 kb on - strand at 844.597 kb on - strand at 844.605 kb on + strand at 844.605 kb on + strand at 844.605 kb on + strand at 844.640 kb on - strand at 844.640 kb on - strand at 844.647 kb on - strand at 844.673 kb on + strand at 844.674 kb on - strand at 844.758 kb on + strand, within AO356_03815 at 844.759 kb on - strand, within AO356_03815 at 844.760 kb on + strand, within AO356_03815 at 844.761 kb on - strand, within AO356_03815 at 844.761 kb on - strand, within AO356_03815 at 844.761 kb on - strand, within AO356_03815 at 844.761 kb on - strand, within AO356_03815 at 844.967 kb on + strand, within AO356_03815 at 844.967 kb on + strand, within AO356_03815 at 844.967 kb on + strand, within AO356_03815 at 844.968 kb on - strand, within AO356_03815 at 845.033 kb on + strand, within AO356_03815 at 845.033 kb on + strand, within AO356_03815 at 845.034 kb on - strand, within AO356_03815 at 845.034 kb on - strand, within AO356_03815 at 845.126 kb on + strand at 845.126 kb on + strand at 845.126 kb on + strand at 845.126 kb on + strand at 845.213 kb on + strand at 845.214 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 841,988 + +1.7 842,085 - +1.0 842,112 + -0.6 842,112 + -0.4 842,112 + -1.3 842,112 + -0.5 842,113 - -0.4 842,113 - +0.4 842,113 - +0.5 842,113 - -0.1 842,116 + -0.4 842,116 + -0.4 842,116 + +0.2 842,117 - +0.2 842,128 + -0.6 842,128 + +0.6 842,129 - +0.1 842,129 - +0.1 842,437 - AO356_03800 0.77 -3.5 842,437 - AO356_03800 0.77 -1.4 842,550 + -2.7 842,551 - +0.3 842,588 - +0.8 842,781 + AO356_03805 0.14 -1.7 842,782 - AO356_03805 0.14 -0.4 842,809 + AO356_03805 0.15 -2.0 842,809 + AO356_03805 0.15 -0.7 842,810 - AO356_03805 0.15 -0.7 842,810 - AO356_03805 0.15 +0.2 842,823 + AO356_03805 0.16 +0.6 842,823 + AO356_03805 0.16 -0.9 842,823 + AO356_03805 0.16 -2.0 842,823 + AO356_03805 0.16 -1.1 842,824 - AO356_03805 0.16 +1.4 842,824 - AO356_03805 0.16 +0.6 842,824 - AO356_03805 0.16 -0.1 842,824 - AO356_03805 0.16 -0.9 842,901 + AO356_03805 0.21 -1.0 842,901 + AO356_03805 0.21 -0.7 842,901 + AO356_03805 0.21 -1.2 842,901 + AO356_03805 0.21 -1.2 842,920 + AO356_03805 0.22 -1.9 842,920 + AO356_03805 0.22 -1.2 842,921 - AO356_03805 0.22 +0.2 842,922 + AO356_03805 0.22 -1.3 842,922 + AO356_03805 0.22 -1.2 842,923 - AO356_03805 0.22 -0.7 842,923 - AO356_03805 0.22 -0.9 842,980 - AO356_03805 0.25 +0.2 843,070 - AO356_03805 0.31 -0.4 843,070 - AO356_03805 0.31 -0.2 843,269 + AO356_03805 0.42 -1.4 843,269 + AO356_03805 0.42 -0.9 843,269 + AO356_03805 0.42 +0.9 843,270 - AO356_03805 0.42 +0.2 843,270 - AO356_03805 0.42 +0.1 843,272 + AO356_03805 0.42 -1.4 843,273 - AO356_03805 0.42 -0.1 843,273 - AO356_03805 0.42 -0.7 843,273 - AO356_03805 0.42 -2.2 843,392 - AO356_03805 0.49 -0.4 843,392 - AO356_03805 0.49 +0.9 843,392 - AO356_03805 0.49 -1.8 843,421 - AO356_03805 0.51 -0.4 843,586 - AO356_03805 0.61 -0.2 843,717 + AO356_03805 0.68 -0.9 843,717 + AO356_03805 0.68 -1.1 843,718 - AO356_03805 0.68 +0.2 843,718 - AO356_03805 0.68 -0.5 843,816 + AO356_03805 0.74 +0.2 843,816 + AO356_03805 0.74 -1.8 843,816 + AO356_03805 0.74 -2.1 843,816 + AO356_03805 0.74 -0.8 843,816 + AO356_03805 0.74 -1.6 843,817 - AO356_03805 0.74 -0.4 843,817 - AO356_03805 0.74 -1.4 843,817 - AO356_03805 0.74 -1.1 843,817 - AO356_03805 0.74 -1.1 843,817 - AO356_03805 0.74 -0.4 843,921 + AO356_03805 0.80 -0.4 843,921 + AO356_03805 0.80 -1.4 843,921 + AO356_03805 0.80 -1.4 843,921 + AO356_03805 0.80 -0.9 843,922 - AO356_03805 0.80 -0.0 843,922 - AO356_03805 0.80 -0.5 843,922 - AO356_03805 0.80 -1.9 843,922 - AO356_03805 0.80 -0.6 843,922 - AO356_03805 0.80 -1.6 844,116 + -2.1 844,116 + -1.8 844,117 - -0.1 844,117 - -1.2 844,117 - -2.7 844,409 + AO356_03810 0.48 +0.1 844,410 - AO356_03810 0.49 +0.6 844,466 + AO356_03810 0.67 -0.5 844,466 + AO356_03810 0.67 +0.4 844,466 + AO356_03810 0.67 +1.0 844,466 + AO356_03810 0.67 +0.4 844,466 + AO356_03810 0.67 -0.6 844,467 - AO356_03810 0.67 -0.8 844,467 - AO356_03810 0.67 +0.2 844,467 - AO356_03810 0.67 +0.1 844,467 - AO356_03810 0.67 -1.2 844,547 - +0.4 844,547 - -1.9 844,590 + +1.4 844,590 + -1.4 844,590 + -0.0 844,590 + -0.0 844,590 + +1.2 844,590 + +1.2 844,591 - -0.1 844,591 - -0.1 844,591 - -0.1 844,591 - -0.7 844,591 - +1.2 844,591 - -0.4 844,591 - -0.4 844,591 - -0.4 844,591 - -0.4 844,591 - +0.2 844,591 - -0.0 844,591 - -0.4 844,596 + -1.3 844,596 + -1.6 844,597 - -1.4 844,597 - +1.1 844,605 + -0.1 844,605 + -0.6 844,605 + +0.6 844,640 - -0.5 844,640 - +0.7 844,647 - -0.2 844,673 + +0.1 844,674 - +0.2 844,758 + AO356_03815 0.20 +1.6 844,759 - AO356_03815 0.20 +1.3 844,760 + AO356_03815 0.20 +1.3 844,761 - AO356_03815 0.20 +1.8 844,761 - AO356_03815 0.20 +0.6 844,761 - AO356_03815 0.20 +1.2 844,761 - AO356_03815 0.20 +1.1 844,967 + AO356_03815 0.66 +1.6 844,967 + AO356_03815 0.66 +1.5 844,967 + AO356_03815 0.66 +0.6 844,968 - AO356_03815 0.66 +2.0 845,033 + AO356_03815 0.81 +1.1 845,033 + AO356_03815 0.81 +0.6 845,034 - AO356_03815 0.81 +1.6 845,034 - AO356_03815 0.81 +1.9 845,126 + +2.3 845,126 + +0.6 845,126 + +0.2 845,126 + +1.6 845,213 + +1.7 845,214 - +1.1
Or see this region's nucleotide sequence