Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_03110 and AO356_03115 are separated by 490 nucleotides AO356_03115 and AO356_03120 are separated by 142 nucleotides
AO356_03110: AO356_03110 - RNA helicase, at 694,906 to 696,579
_03110
AO356_03115: AO356_03115 - spermidine synthase, at 697,070 to 697,825
_03115
AO356_03120: AO356_03120 - phospho-2-dehydro-3-deoxyheptonate aldolase, at 697,968 to 699,314
_03120
Position (kb) 697
698 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 696.592 kb on - strand at 696.803 kb on - strand at 696.803 kb on - strand at 696.803 kb on - strand at 696.803 kb on - strand at 696.805 kb on - strand at 696.805 kb on - strand at 696.890 kb on + strand at 696.974 kb on + strand at 696.975 kb on - strand at 696.975 kb on - strand at 696.975 kb on - strand at 696.975 kb on - strand at 697.047 kb on - strand at 697.114 kb on - strand at 697.233 kb on + strand, within AO356_03115 at 697.245 kb on + strand, within AO356_03115 at 697.245 kb on + strand, within AO356_03115 at 697.246 kb on - strand, within AO356_03115 at 697.246 kb on - strand, within AO356_03115 at 697.395 kb on + strand, within AO356_03115 at 697.395 kb on + strand, within AO356_03115 at 697.395 kb on + strand, within AO356_03115 at 697.467 kb on + strand, within AO356_03115 at 697.467 kb on + strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.468 kb on - strand, within AO356_03115 at 697.665 kb on + strand, within AO356_03115 at 697.665 kb on + strand, within AO356_03115 at 697.665 kb on + strand, within AO356_03115 at 697.665 kb on + strand, within AO356_03115 at 697.666 kb on - strand, within AO356_03115 at 697.666 kb on - strand, within AO356_03115 at 697.666 kb on - strand, within AO356_03115 at 697.666 kb on - strand, within AO356_03115 at 697.666 kb on - strand, within AO356_03115 at 697.666 kb on - strand, within AO356_03115 at 697.666 kb on - strand, within AO356_03115 at 697.666 kb on - strand, within AO356_03115 at 697.671 kb on + strand, within AO356_03115 at 697.672 kb on - strand, within AO356_03115 at 697.672 kb on - strand, within AO356_03115 at 697.672 kb on - strand, within AO356_03115 at 697.760 kb on + strand at 697.760 kb on + strand at 697.915 kb on + strand at 697.915 kb on + strand at 697.916 kb on - strand at 697.916 kb on - strand at 697.918 kb on + strand at 697.918 kb on + strand at 697.918 kb on + strand at 697.918 kb on + strand at 697.918 kb on + strand at 697.919 kb on - strand at 697.919 kb on - strand at 697.919 kb on - strand at 697.919 kb on - strand at 697.929 kb on + strand at 697.930 kb on - strand at 697.930 kb on - strand at 697.949 kb on - strand at 697.949 kb on - strand at 697.949 kb on - strand at 697.957 kb on + strand at 697.957 kb on + strand at 697.958 kb on - strand at 697.958 kb on - strand at 698.439 kb on + strand, within AO356_03120 at 698.562 kb on + strand, within AO356_03120 at 698.563 kb on - strand, within AO356_03120 at 698.563 kb on - strand, within AO356_03120 at 698.685 kb on + strand, within AO356_03120 at 698.685 kb on + strand, within AO356_03120 at 698.685 kb on + strand, within AO356_03120 at 698.686 kb on - strand, within AO356_03120 at 698.727 kb on + strand, within AO356_03120 at 698.728 kb on - strand, within AO356_03120 at 698.728 kb on - strand, within AO356_03120 at 698.728 kb on - strand, within AO356_03120 at 698.730 kb on + strand, within AO356_03120 at 698.730 kb on + strand, within AO356_03120 at 698.730 kb on + strand, within AO356_03120 at 698.730 kb on + strand, within AO356_03120 at 698.731 kb on - strand, within AO356_03120 at 698.731 kb on - strand, within AO356_03120
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 696,592 - -1.4 696,803 - -0.8 696,803 - +0.2 696,803 - -1.1 696,803 - +1.2 696,805 - -2.4 696,805 - -0.1 696,890 + +0.6 696,974 + -0.9 696,975 - +0.5 696,975 - -1.0 696,975 - -0.4 696,975 - +0.2 697,047 - -0.0 697,114 - +0.2 697,233 + AO356_03115 0.22 +0.5 697,245 + AO356_03115 0.23 +0.2 697,245 + AO356_03115 0.23 +1.4 697,246 - AO356_03115 0.23 -0.3 697,246 - AO356_03115 0.23 +1.0 697,395 + AO356_03115 0.43 -0.6 697,395 + AO356_03115 0.43 -0.2 697,395 + AO356_03115 0.43 -0.0 697,467 + AO356_03115 0.53 -1.6 697,467 + AO356_03115 0.53 -0.0 697,468 - AO356_03115 0.53 +0.2 697,468 - AO356_03115 0.53 -0.1 697,468 - AO356_03115 0.53 +0.1 697,468 - AO356_03115 0.53 +0.2 697,468 - AO356_03115 0.53 +0.4 697,468 - AO356_03115 0.53 +2.0 697,468 - AO356_03115 0.53 -2.1 697,468 - AO356_03115 0.53 +0.2 697,468 - AO356_03115 0.53 -0.1 697,468 - AO356_03115 0.53 +0.2 697,468 - AO356_03115 0.53 -0.5 697,665 + AO356_03115 0.79 -0.2 697,665 + AO356_03115 0.79 -1.1 697,665 + AO356_03115 0.79 +0.9 697,665 + AO356_03115 0.79 -0.0 697,666 - AO356_03115 0.79 +0.7 697,666 - AO356_03115 0.79 +1.6 697,666 - AO356_03115 0.79 +0.2 697,666 - AO356_03115 0.79 -0.4 697,666 - AO356_03115 0.79 -0.1 697,666 - AO356_03115 0.79 +1.2 697,666 - AO356_03115 0.79 +0.7 697,666 - AO356_03115 0.79 +1.5 697,671 + AO356_03115 0.79 -0.7 697,672 - AO356_03115 0.80 +0.6 697,672 - AO356_03115 0.80 +1.0 697,672 - AO356_03115 0.80 -1.4 697,760 + +0.5 697,760 + -0.4 697,915 + -1.1 697,915 + -0.6 697,916 - +1.1 697,916 - +0.5 697,918 + +0.2 697,918 + -0.4 697,918 + -1.7 697,918 + +1.0 697,918 + -3.2 697,919 - -1.5 697,919 - -0.4 697,919 - +0.4 697,919 - -0.6 697,929 + -0.4 697,930 - -0.4 697,930 - +0.3 697,949 - +1.4 697,949 - -0.4 697,949 - +0.4 697,957 + +0.2 697,957 + -0.0 697,958 - +0.5 697,958 - +1.0 698,439 + AO356_03120 0.35 -0.7 698,562 + AO356_03120 0.44 -0.2 698,563 - AO356_03120 0.44 -0.1 698,563 - AO356_03120 0.44 +0.4 698,685 + AO356_03120 0.53 +0.6 698,685 + AO356_03120 0.53 +0.8 698,685 + AO356_03120 0.53 +0.3 698,686 - AO356_03120 0.53 -0.4 698,727 + AO356_03120 0.56 +1.0 698,728 - AO356_03120 0.56 +0.2 698,728 - AO356_03120 0.56 +0.8 698,728 - AO356_03120 0.56 -0.8 698,730 + AO356_03120 0.57 +0.1 698,730 + AO356_03120 0.57 -0.2 698,730 + AO356_03120 0.57 -0.7 698,730 + AO356_03120 0.57 -1.4 698,731 - AO356_03120 0.57 +0.4 698,731 - AO356_03120 0.57 +0.5
Or see this region's nucleotide sequence