Experiment: 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO356_01190 and AO356_01195 overlap by 4 nucleotides AO356_01195 and AO356_01200 are separated by 230 nucleotides
AO356_01190: AO356_01190 - polysaccharide deacetylase, at 272,704 to 273,708
_01190
AO356_01195: AO356_01195 - polysaccharide biosynthesis protein, at 273,705 to 275,009
_01195
AO356_01200: AO356_01200 - hypothetical protein, at 275,240 to 277,189
_01200
Position (kb) 273
274
275
276 Strain fitness (log2 ratio) -1
0
1 at 272.747 kb on - strand at 273.253 kb on + strand, within AO356_01190 at 273.253 kb on + strand, within AO356_01190 at 273.253 kb on + strand, within AO356_01190 at 273.505 kb on + strand, within AO356_01190 at 273.505 kb on + strand, within AO356_01190 at 273.505 kb on + strand, within AO356_01190 at 273.506 kb on - strand, within AO356_01190 at 273.506 kb on - strand, within AO356_01190 at 273.506 kb on - strand, within AO356_01190 at 273.671 kb on + strand at 273.671 kb on + strand at 273.956 kb on - strand, within AO356_01195 at 274.063 kb on + strand, within AO356_01195 at 274.064 kb on - strand, within AO356_01195 at 274.455 kb on + strand, within AO356_01195 at 274.723 kb on - strand, within AO356_01195 at 274.839 kb on + strand, within AO356_01195 at 274.911 kb on + strand at 274.912 kb on - strand at 274.912 kb on - strand at 275.408 kb on + strand at 275.409 kb on - strand at 275.636 kb on + strand, within AO356_01200 at 275.636 kb on + strand, within AO356_01200 at 275.637 kb on - strand, within AO356_01200 at 275.765 kb on + strand, within AO356_01200 at 275.984 kb on + strand, within AO356_01200 at 275.984 kb on + strand, within AO356_01200 at 275.985 kb on - strand, within AO356_01200 at 275.985 kb on - strand, within AO356_01200 at 275.993 kb on + strand, within AO356_01200 at 275.993 kb on + strand, within AO356_01200 at 275.994 kb on - strand, within AO356_01200
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine remove 272,747 - +0.2 273,253 + AO356_01190 0.55 -0.8 273,253 + AO356_01190 0.55 +0.7 273,253 + AO356_01190 0.55 -0.1 273,505 + AO356_01190 0.80 -0.4 273,505 + AO356_01190 0.80 -0.1 273,505 + AO356_01190 0.80 -0.2 273,506 - AO356_01190 0.80 +0.5 273,506 - AO356_01190 0.80 -1.1 273,506 - AO356_01190 0.80 -0.4 273,671 + +1.6 273,671 + +0.7 273,956 - AO356_01195 0.19 -1.1 274,063 + AO356_01195 0.27 -1.3 274,064 - AO356_01195 0.28 -1.7 274,455 + AO356_01195 0.57 -1.2 274,723 - AO356_01195 0.78 +1.6 274,839 + AO356_01195 0.87 +0.6 274,911 + -0.8 274,912 - -0.8 274,912 - -1.2 275,408 + -1.2 275,409 - -0.2 275,636 + AO356_01200 0.20 +0.3 275,636 + AO356_01200 0.20 -0.0 275,637 - AO356_01200 0.20 +0.0 275,765 + AO356_01200 0.27 +0.0 275,984 + AO356_01200 0.38 +0.3 275,984 + AO356_01200 0.38 -0.4 275,985 - AO356_01200 0.38 +0.2 275,985 - AO356_01200 0.38 +0.7 275,993 + AO356_01200 0.39 -1.0 275,993 + AO356_01200 0.39 -0.3 275,994 - AO356_01200 0.39 -0.2
Or see this region's nucleotide sequence