Experiment: SBM - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiDE and thiC overlap by 1 nucleotides thiC and SO2446 are separated by 435 nucleotides
SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537
thiDE
SO2445: thiC - thiamin biosynthesis protein ThiC (NCBI ptt file), at 2,558,537 to 2,560,702
thiC
SO2446: SO2446 - hypothetical protein (NCBI ptt file), at 2,561,138 to 2,562,205
SO2446
Position (kb)
2558
2559
2560
2561 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2557.552 kb on + strand, within thiDE at 2557.555 kb on - strand, within thiDE at 2557.676 kb on + strand, within thiDE at 2557.690 kb on + strand, within thiDE at 2557.723 kb on + strand, within thiDE at 2557.729 kb on + strand, within thiDE at 2557.742 kb on + strand, within thiDE at 2557.760 kb on - strand, within thiDE at 2557.770 kb on + strand, within thiDE at 2557.778 kb on - strand, within thiDE at 2557.782 kb on + strand, within thiDE at 2557.790 kb on - strand, within thiDE at 2557.794 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2557.874 kb on + strand, within thiDE at 2558.275 kb on - strand, within thiDE at 2558.316 kb on - strand, within thiDE at 2558.535 kb on + strand at 2558.543 kb on - strand at 2558.580 kb on + strand at 2558.613 kb on + strand at 2558.615 kb on - strand at 2558.863 kb on - strand, within thiC at 2558.898 kb on - strand, within thiC at 2558.905 kb on - strand, within thiC at 2559.185 kb on + strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.193 kb on - strand, within thiC at 2559.278 kb on - strand, within thiC at 2559.302 kb on - strand, within thiC at 2559.362 kb on + strand, within thiC at 2559.408 kb on + strand, within thiC at 2559.578 kb on + strand, within thiC at 2559.870 kb on - strand, within thiC at 2559.992 kb on - strand, within thiC at 2560.056 kb on - strand, within thiC at 2560.207 kb on - strand, within thiC at 2560.379 kb on - strand, within thiC at 2560.430 kb on - strand, within thiC at 2560.439 kb on + strand, within thiC at 2560.502 kb on - strand at 2560.614 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.687 kb on + strand at 2560.695 kb on - strand at 2560.750 kb on + strand at 2560.829 kb on - strand at 2560.937 kb on + strand at 2560.967 kb on + strand at 2561.166 kb on + strand at 2561.174 kb on - strand at 2561.246 kb on - strand, within SO2446 at 2561.251 kb on - strand, within SO2446 at 2561.343 kb on + strand, within SO2446 at 2561.367 kb on + strand, within SO2446 at 2561.402 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand, within SO2446 at 2561.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - no RNA protect remove 2,557,552 + thiDE SO2444 0.38 -0.7 2,557,555 - thiDE SO2444 0.38 -0.7 2,557,676 + thiDE SO2444 0.45 +0.1 2,557,690 + thiDE SO2444 0.46 -0.7 2,557,723 + thiDE SO2444 0.48 -0.9 2,557,729 + thiDE SO2444 0.49 -1.2 2,557,742 + thiDE SO2444 0.50 -0.7 2,557,760 - thiDE SO2444 0.51 -0.2 2,557,770 + thiDE SO2444 0.51 -1.9 2,557,778 - thiDE SO2444 0.52 -0.5 2,557,782 + thiDE SO2444 0.52 -1.0 2,557,790 - thiDE SO2444 0.53 -1.1 2,557,794 + thiDE SO2444 0.53 +0.7 2,557,874 + thiDE SO2444 0.58 -2.5 2,557,874 + thiDE SO2444 0.58 -1.4 2,558,275 - thiDE SO2444 0.83 +0.1 2,558,316 - thiDE SO2444 0.86 -1.0 2,558,535 + -0.9 2,558,543 - -0.5 2,558,580 + -0.3 2,558,613 + -0.6 2,558,615 - -1.1 2,558,863 - thiC SO2445 0.15 -1.1 2,558,898 - thiC SO2445 0.17 -0.6 2,558,905 - thiC SO2445 0.17 -1.7 2,559,185 + thiC SO2445 0.30 -1.2 2,559,193 - thiC SO2445 0.30 -2.1 2,559,193 - thiC SO2445 0.30 -1.3 2,559,278 - thiC SO2445 0.34 -0.7 2,559,302 - thiC SO2445 0.35 -1.5 2,559,362 + thiC SO2445 0.38 -2.1 2,559,408 + thiC SO2445 0.40 -1.2 2,559,578 + thiC SO2445 0.48 -0.9 2,559,870 - thiC SO2445 0.62 -0.5 2,559,992 - thiC SO2445 0.67 -2.3 2,560,056 - thiC SO2445 0.70 -1.0 2,560,207 - thiC SO2445 0.77 -0.6 2,560,379 - thiC SO2445 0.85 -1.6 2,560,430 - thiC SO2445 0.87 -1.3 2,560,439 + thiC SO2445 0.88 -0.4 2,560,502 - -0.9 2,560,614 + -1.7 2,560,687 + -1.3 2,560,687 + -1.6 2,560,687 + -0.5 2,560,687 + -3.0 2,560,695 - -1.7 2,560,750 + -0.1 2,560,829 - +1.5 2,560,937 + -0.5 2,560,967 + +0.1 2,561,166 + +0.1 2,561,174 - -0.8 2,561,246 - SO2446 0.10 +1.1 2,561,251 - SO2446 0.11 +0.6 2,561,343 + SO2446 0.19 +0.6 2,561,367 + SO2446 0.21 -0.2 2,561,402 + SO2446 0.25 -0.6 2,561,553 + SO2446 0.39 -0.1 2,561,553 + SO2446 0.39 -0.9 2,561,553 + SO2446 0.39 -0.0 2,561,553 + SO2446 0.39 +0.5 2,561,553 + SO2446 0.39 +1.8 2,561,553 + -0.5
Or see this region's nucleotide sequence