Strain Fitness in Shewanella oneidensis MR-1 around SO2442

Experiment: SBM - no RNA protect

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthiH and thiG overlap by 4 nucleotidesthiG and thiS are separated by 1 nucleotidesthiS and thiF overlap by 4 nucleotidesthiF and thiDE overlap by 4 nucleotides SO2440: thiH - thiH protein (NCBI ptt file), at 2,553,968 to 2,555,095 thiH SO2441: thiG - thiG protein (NCBI ptt file), at 2,555,092 to 2,555,856 thiG SO2442: thiS - thiS protein, putative (NCBI ptt file), at 2,555,858 to 2,556,061 thiS SO2443: thiF - thiF protein, putative (NCBI ptt file), at 2,556,058 to 2,556,960 thiF SO2444: thiDE - phosphomethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase, putative (NCBI ptt file), at 2,556,957 to 2,558,537 thiDE Position (kb) 2555 2556 2557Strain fitness (log2 ratio) -3 -2 -1 0 1at 2554.866 kb on + strand, within thiHat 2554.866 kb on + strand, within thiHat 2554.874 kb on - strand, within thiHat 2554.874 kb on - strand, within thiHat 2554.874 kb on - strand, within thiHat 2554.914 kb on + strand, within thiHat 2555.122 kb on + strandat 2555.122 kb on + strandat 2555.147 kb on + strandat 2555.156 kb on - strandat 2555.243 kb on + strand, within thiGat 2555.243 kb on + strand, within thiGat 2555.251 kb on - strand, within thiGat 2555.410 kb on + strand, within thiGat 2555.433 kb on - strand, within thiGat 2555.489 kb on - strand, within thiGat 2555.522 kb on - strand, within thiGat 2555.563 kb on + strand, within thiGat 2555.580 kb on + strand, within thiGat 2555.855 kb on + strandat 2555.919 kb on - strand, within thiSat 2556.483 kb on + strand, within thiFat 2556.680 kb on + strand, within thiFat 2556.680 kb on + strand, within thiFat 2556.680 kb on + strand, within thiFat 2556.738 kb on + strand, within thiFat 2556.738 kb on + strand, within thiFat 2556.918 kb on - strandat 2556.971 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SBM - no RNA protect
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2,554,866 + thiH SO2440 0.80 -1.6
2,554,866 + thiH SO2440 0.80 -1.3
2,554,874 - thiH SO2440 0.80 -2.8
2,554,874 - thiH SO2440 0.80 -2.4
2,554,874 - thiH SO2440 0.80 -1.6
2,554,914 + thiH SO2440 0.84 -1.1
2,555,122 + +1.1
2,555,122 + -0.3
2,555,147 + -1.3
2,555,156 - -0.3
2,555,243 + thiG SO2441 0.20 -1.2
2,555,243 + thiG SO2441 0.20 -1.3
2,555,251 - thiG SO2441 0.21 -1.7
2,555,410 + thiG SO2441 0.42 -0.9
2,555,433 - thiG SO2441 0.45 -2.4
2,555,489 - thiG SO2441 0.52 -1.3
2,555,522 - thiG SO2441 0.56 +0.1
2,555,563 + thiG SO2441 0.62 -0.0
2,555,580 + thiG SO2441 0.64 -1.3
2,555,855 + -1.0
2,555,919 - thiS SO2442 0.30 -1.1
2,556,483 + thiF SO2443 0.47 -0.3
2,556,680 + thiF SO2443 0.69 -0.9
2,556,680 + thiF SO2443 0.69 -1.7
2,556,680 + thiF SO2443 0.69 -1.0
2,556,738 + thiF SO2443 0.75 -0.5
2,556,738 + thiF SO2443 0.75 +0.1
2,556,918 - -2.6
2,556,971 - -0.8

Or see this region's nucleotide sequence