Experiment: SBM - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glgX and SO1496 are separated by 2 nucleotides SO1496 and glgC are separated by 119 nucleotides
SO1495: glgX - glycogen operon protein (NCBI ptt file), at 1,562,508 to 1,564,760
glgX
SO1496: SO1496 - glycogen phosphorylase family protein (NCBI ptt file), at 1,564,763 to 1,567,276
SO1496
SO1498: glgC - glucose-1-phosphate adenylyltransferase (NCBI ptt file), at 1,567,396 to 1,568,658
glgC
Position (kb)
1564
1565
1566
1567
1568 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1563.991 kb on - strand, within glgX at 1564.031 kb on + strand, within glgX at 1564.031 kb on + strand, within glgX at 1564.084 kb on - strand, within glgX at 1564.132 kb on + strand, within glgX at 1564.258 kb on + strand, within glgX at 1564.331 kb on + strand, within glgX at 1564.368 kb on + strand, within glgX at 1564.375 kb on - strand, within glgX at 1564.376 kb on - strand, within glgX at 1564.376 kb on - strand, within glgX at 1564.385 kb on + strand, within glgX at 1564.430 kb on + strand, within glgX at 1564.483 kb on + strand, within glgX at 1564.496 kb on - strand, within glgX at 1564.585 kb on + strand at 1564.593 kb on - strand at 1564.657 kb on + strand at 1564.688 kb on + strand at 1564.688 kb on + strand at 1564.688 kb on + strand at 1564.696 kb on - strand at 1564.696 kb on - strand at 1564.979 kb on - strand at 1565.079 kb on - strand, within SO1496 at 1565.129 kb on + strand, within SO1496 at 1565.137 kb on - strand, within SO1496 at 1565.137 kb on - strand, within SO1496 at 1565.151 kb on + strand, within SO1496 at 1565.151 kb on + strand, within SO1496 at 1565.159 kb on - strand, within SO1496 at 1565.186 kb on + strand, within SO1496 at 1565.186 kb on + strand, within SO1496 at 1565.194 kb on - strand, within SO1496 at 1565.243 kb on + strand, within SO1496 at 1565.348 kb on + strand, within SO1496 at 1565.353 kb on + strand, within SO1496 at 1565.381 kb on - strand, within SO1496 at 1565.421 kb on - strand, within SO1496 at 1565.490 kb on + strand, within SO1496 at 1565.593 kb on + strand, within SO1496 at 1565.593 kb on + strand, within SO1496 at 1565.612 kb on - strand, within SO1496 at 1565.647 kb on + strand, within SO1496 at 1565.676 kb on + strand, within SO1496 at 1565.813 kb on + strand, within SO1496 at 1565.816 kb on - strand, within SO1496 at 1565.816 kb on - strand, within SO1496 at 1565.909 kb on + strand, within SO1496 at 1565.939 kb on + strand, within SO1496 at 1565.955 kb on - strand, within SO1496 at 1565.977 kb on - strand, within SO1496 at 1565.977 kb on - strand, within SO1496 at 1566.040 kb on + strand, within SO1496 at 1566.048 kb on - strand, within SO1496 at 1566.053 kb on - strand, within SO1496 at 1566.054 kb on + strand, within SO1496 at 1566.072 kb on - strand, within SO1496 at 1566.246 kb on + strand, within SO1496 at 1566.258 kb on + strand, within SO1496 at 1566.259 kb on + strand, within SO1496 at 1566.272 kb on - strand, within SO1496 at 1566.282 kb on - strand, within SO1496 at 1566.304 kb on - strand, within SO1496 at 1566.304 kb on - strand, within SO1496 at 1566.317 kb on - strand, within SO1496 at 1566.319 kb on + strand, within SO1496 at 1566.319 kb on + strand, within SO1496 at 1566.319 kb on + strand, within SO1496 at 1566.340 kb on - strand, within SO1496 at 1566.399 kb on + strand, within SO1496 at 1566.453 kb on - strand, within SO1496 at 1566.468 kb on + strand, within SO1496 at 1566.468 kb on + strand, within SO1496 at 1566.523 kb on + strand, within SO1496 at 1566.523 kb on + strand, within SO1496 at 1566.573 kb on + strand, within SO1496 at 1566.795 kb on - strand, within SO1496 at 1566.795 kb on - strand, within SO1496 at 1566.818 kb on - strand, within SO1496 at 1566.853 kb on + strand, within SO1496 at 1566.853 kb on + strand, within SO1496 at 1566.853 kb on + strand, within SO1496 at 1566.872 kb on - strand, within SO1496 at 1566.941 kb on - strand, within SO1496 at 1566.943 kb on + strand, within SO1496 at 1567.035 kb on + strand at 1567.043 kb on - strand at 1567.279 kb on - strand at 1567.296 kb on + strand at 1567.350 kb on + strand at 1567.358 kb on - strand at 1567.373 kb on - strand at 1567.475 kb on + strand at 1567.475 kb on + strand at 1567.874 kb on + strand, within glgC at 1568.028 kb on + strand, within glgC at 1568.137 kb on + strand, within glgC at 1568.137 kb on + strand, within glgC at 1568.159 kb on - strand, within glgC at 1568.252 kb on + strand, within glgC at 1568.252 kb on + strand, within glgC at 1568.260 kb on - strand, within glgC
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - no RNA protect remove 1,563,991 - glgX SO1495 0.66 +1.2 1,564,031 + glgX SO1495 0.68 +0.2 1,564,031 + glgX SO1495 0.68 -0.2 1,564,084 - glgX SO1495 0.70 -0.1 1,564,132 + glgX SO1495 0.72 +1.0 1,564,258 + glgX SO1495 0.78 -1.0 1,564,331 + glgX SO1495 0.81 -0.0 1,564,368 + glgX SO1495 0.83 +0.1 1,564,375 - glgX SO1495 0.83 -0.5 1,564,376 - glgX SO1495 0.83 +0.2 1,564,376 - glgX SO1495 0.83 +0.4 1,564,385 + glgX SO1495 0.83 -0.3 1,564,430 + glgX SO1495 0.85 +0.6 1,564,483 + glgX SO1495 0.88 -0.3 1,564,496 - glgX SO1495 0.88 -1.5 1,564,585 + -0.8 1,564,593 - +0.4 1,564,657 + -0.6 1,564,688 + +0.7 1,564,688 + +0.4 1,564,688 + -1.5 1,564,696 - +0.2 1,564,696 - -0.4 1,564,979 - +0.7 1,565,079 - SO1496 0.13 +0.2 1,565,129 + SO1496 0.15 +0.7 1,565,137 - SO1496 0.15 +1.1 1,565,137 - SO1496 0.15 -0.3 1,565,151 + SO1496 0.15 +0.9 1,565,151 + SO1496 0.15 -0.3 1,565,159 - SO1496 0.16 -0.4 1,565,186 + SO1496 0.17 -0.1 1,565,186 + SO1496 0.17 -0.4 1,565,194 - SO1496 0.17 +0.0 1,565,243 + SO1496 0.19 +1.0 1,565,348 + SO1496 0.23 -0.7 1,565,353 + SO1496 0.23 -0.5 1,565,381 - SO1496 0.25 +1.0 1,565,421 - SO1496 0.26 +0.3 1,565,490 + SO1496 0.29 -0.1 1,565,593 + SO1496 0.33 -0.6 1,565,593 + SO1496 0.33 +0.6 1,565,612 - SO1496 0.34 +1.8 1,565,647 + SO1496 0.35 -0.1 1,565,676 + SO1496 0.36 +0.4 1,565,813 + SO1496 0.42 +0.3 1,565,816 - SO1496 0.42 +0.7 1,565,816 - SO1496 0.42 -0.1 1,565,909 + SO1496 0.46 -0.7 1,565,939 + SO1496 0.47 +0.8 1,565,955 - SO1496 0.47 +0.3 1,565,977 - SO1496 0.48 +0.2 1,565,977 - SO1496 0.48 -0.6 1,566,040 + SO1496 0.51 +0.7 1,566,048 - SO1496 0.51 +1.4 1,566,053 - SO1496 0.51 +0.8 1,566,054 + SO1496 0.51 +0.6 1,566,072 - SO1496 0.52 +1.8 1,566,246 + SO1496 0.59 +0.4 1,566,258 + SO1496 0.59 +0.6 1,566,259 + SO1496 0.60 -0.7 1,566,272 - SO1496 0.60 +0.2 1,566,282 - SO1496 0.60 -1.6 1,566,304 - SO1496 0.61 +0.4 1,566,304 - SO1496 0.61 -0.3 1,566,317 - SO1496 0.62 -1.1 1,566,319 + SO1496 0.62 -0.4 1,566,319 + SO1496 0.62 -1.4 1,566,319 + SO1496 0.62 +2.8 1,566,340 - SO1496 0.63 +0.0 1,566,399 + SO1496 0.65 +0.1 1,566,453 - SO1496 0.67 +0.8 1,566,468 + SO1496 0.68 +0.2 1,566,468 + SO1496 0.68 +0.2 1,566,523 + SO1496 0.70 +0.5 1,566,523 + SO1496 0.70 +0.5 1,566,573 + SO1496 0.72 +0.6 1,566,795 - SO1496 0.81 +0.6 1,566,795 - SO1496 0.81 -0.6 1,566,818 - SO1496 0.82 +0.2 1,566,853 + SO1496 0.83 -0.8 1,566,853 + SO1496 0.83 +0.4 1,566,853 + SO1496 0.83 +0.8 1,566,872 - SO1496 0.84 +0.0 1,566,941 - SO1496 0.87 -0.0 1,566,943 + SO1496 0.87 +0.4 1,567,035 + -0.6 1,567,043 - +0.0 1,567,279 - +1.5 1,567,296 + -0.8 1,567,350 + +0.9 1,567,358 - -0.7 1,567,373 - +0.3 1,567,475 + -0.6 1,567,475 + +0.4 1,567,874 + glgC SO1498 0.38 +0.1 1,568,028 + glgC SO1498 0.50 +1.6 1,568,137 + glgC SO1498 0.59 -0.4 1,568,137 + glgC SO1498 0.59 -0.0 1,568,159 - glgC SO1498 0.60 +0.1 1,568,252 + glgC SO1498 0.68 -0.8 1,568,252 + glgC SO1498 0.68 -0.0 1,568,260 - glgC SO1498 0.68 +0.4
Or see this region's nucleotide sequence