Experiment: SBM - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO1136 and SO1137 are separated by 213 nucleotides SO1137 and fklB are separated by 373 nucleotides
SO1136: SO1136 - ATP-dependent RNA helicase, DEAD box family (NCBI ptt file), at 1,177,348 to 1,178,691
SO1136
SO1137: SO1137 - conserved hypothetical protein (NCBI ptt file), at 1,178,905 to 1,179,714
SO1137
SO1139: fklB - peptidyl-prolyl cis-trans isomerase FklB (NCBI ptt file), at 1,180,088 to 1,180,705
fklB
Position (kb)
1178
1179
1180 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1177.938 kb on + strand, within SO1136 at 1177.996 kb on + strand, within SO1136 at 1178.052 kb on - strand, within SO1136 at 1178.052 kb on - strand, within SO1136 at 1178.109 kb on + strand, within SO1136 at 1178.109 kb on + strand, within SO1136 at 1178.117 kb on - strand, within SO1136 at 1178.229 kb on + strand, within SO1136 at 1178.237 kb on - strand, within SO1136 at 1178.366 kb on - strand, within SO1136 at 1178.396 kb on + strand, within SO1136 at 1178.457 kb on - strand, within SO1136 at 1178.680 kb on + strand at 1178.681 kb on - strand at 1178.700 kb on - strand at 1178.729 kb on - strand at 1178.776 kb on + strand at 1178.924 kb on + strand at 1178.924 kb on + strand at 1178.937 kb on - strand at 1178.959 kb on - strand at 1178.968 kb on + strand at 1179.186 kb on + strand, within SO1137 at 1179.202 kb on - strand, within SO1137 at 1179.290 kb on - strand, within SO1137 at 1179.474 kb on - strand, within SO1137 at 1179.493 kb on + strand, within SO1137 at 1179.536 kb on + strand, within SO1137 at 1179.536 kb on + strand, within SO1137 at 1179.536 kb on + strand, within SO1137 at 1179.544 kb on - strand, within SO1137 at 1179.544 kb on - strand, within SO1137 at 1179.546 kb on - strand, within SO1137 at 1179.581 kb on + strand, within SO1137 at 1179.651 kb on + strand at 1179.704 kb on + strand at 1180.029 kb on + strand at 1180.080 kb on + strand at 1180.205 kb on + strand, within fklB at 1180.288 kb on + strand, within fklB at 1180.296 kb on - strand, within fklB at 1180.298 kb on + strand, within fklB at 1180.371 kb on + strand, within fklB at 1180.391 kb on + strand, within fklB at 1180.406 kb on + strand, within fklB at 1180.406 kb on + strand, within fklB at 1180.425 kb on + strand, within fklB at 1180.425 kb on + strand, within fklB at 1180.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - no RNA protect remove 1,177,938 + SO1136 0.44 -0.6 1,177,996 + SO1136 0.48 +0.6 1,178,052 - SO1136 0.52 +0.2 1,178,052 - SO1136 0.52 +1.1 1,178,109 + SO1136 0.57 +0.1 1,178,109 + SO1136 0.57 -1.1 1,178,117 - SO1136 0.57 -0.6 1,178,229 + SO1136 0.66 +0.2 1,178,237 - SO1136 0.66 +0.1 1,178,366 - SO1136 0.76 -0.2 1,178,396 + SO1136 0.78 +1.0 1,178,457 - SO1136 0.83 +0.8 1,178,680 + -3.3 1,178,681 - +0.3 1,178,700 - -0.2 1,178,729 - +0.4 1,178,776 + +0.2 1,178,924 + -0.0 1,178,924 + -0.6 1,178,937 - +0.2 1,178,959 - -0.4 1,178,968 + +1.1 1,179,186 + SO1137 0.35 -0.6 1,179,202 - SO1137 0.37 -0.2 1,179,290 - SO1137 0.48 -1.4 1,179,474 - SO1137 0.70 +0.2 1,179,493 + SO1137 0.73 -0.8 1,179,536 + SO1137 0.78 -0.7 1,179,536 + SO1137 0.78 +0.7 1,179,536 + SO1137 0.78 -0.2 1,179,544 - SO1137 0.79 -0.6 1,179,544 - SO1137 0.79 -0.1 1,179,546 - SO1137 0.79 -0.3 1,179,581 + SO1137 0.83 -0.0 1,179,651 + -0.4 1,179,704 + +0.2 1,180,029 + -0.3 1,180,080 + -0.2 1,180,205 + fklB SO1139 0.19 +0.1 1,180,288 + fklB SO1139 0.32 -0.8 1,180,296 - fklB SO1139 0.34 +1.2 1,180,298 + fklB SO1139 0.34 +0.0 1,180,371 + fklB SO1139 0.46 -1.0 1,180,391 + fklB SO1139 0.49 +0.2 1,180,406 + fklB SO1139 0.51 -0.1 1,180,406 + fklB SO1139 0.51 +0.6 1,180,425 + fklB SO1139 0.55 +1.2 1,180,425 + fklB SO1139 0.55 -1.1 1,180,647 + +0.2
Or see this region's nucleotide sequence