Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS25230

Experiment: Carbon source 2-Deoxy-D-Ribose 20 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS25220 and RR42_RS25225 are separated by 10 nucleotidesRR42_RS25225 and RR42_RS25230 overlap by 1 nucleotidesRR42_RS25230 and RR42_RS25235 are separated by 351 nucleotides RR42_RS25220: RR42_RS25220 - NADP transhydrogenase subunit alpha, at 999,456 to 1,000,574 _RS25220 RR42_RS25225: RR42_RS25225 - NAD(P) transhydrogenase, at 1,000,585 to 1,000,908 _RS25225 RR42_RS25230: RR42_RS25230 - NAD synthetase, at 1,000,908 to 1,002,377 _RS25230 RR42_RS25235: RR42_RS25235 - DSBA oxidoreductase, at 1,002,729 to 1,003,331 _RS25235 Position (kb) 1000 1001 1002 1003Strain fitness (log2 ratio) -2 -1 0 1 2at 1000.597 kb on - strandat 1000.639 kb on + strand, within RR42_RS25225at 1000.640 kb on - strand, within RR42_RS25225at 1000.881 kb on + strandat 1000.882 kb on - strandat 1000.944 kb on + strandat 1000.944 kb on + strandat 1001.185 kb on + strand, within RR42_RS25230at 1001.428 kb on - strand, within RR42_RS25230at 1001.652 kb on + strand, within RR42_RS25230at 1001.653 kb on - strand, within RR42_RS25230at 1001.752 kb on - strand, within RR42_RS25230at 1001.752 kb on - strand, within RR42_RS25230at 1001.752 kb on - strand, within RR42_RS25230at 1002.004 kb on + strand, within RR42_RS25230at 1002.005 kb on - strand, within RR42_RS25230at 1002.009 kb on + strand, within RR42_RS25230at 1002.010 kb on - strand, within RR42_RS25230at 1002.010 kb on - strand, within RR42_RS25230at 1002.048 kb on - strand, within RR42_RS25230at 1002.048 kb on - strand, within RR42_RS25230at 1002.081 kb on + strand, within RR42_RS25230at 1002.303 kb on + strandat 1002.513 kb on + strandat 1002.514 kb on - strandat 1002.514 kb on - strandat 1002.517 kb on + strandat 1002.517 kb on + strandat 1002.517 kb on + strandat 1002.552 kb on - strandat 1002.552 kb on - strandat 1002.653 kb on + strandat 1002.654 kb on - strandat 1002.791 kb on + strand, within RR42_RS25235at 1002.791 kb on + strand, within RR42_RS25235at 1002.791 kb on + strand, within RR42_RS25235at 1002.791 kb on + strand, within RR42_RS25235at 1002.792 kb on - strand, within RR42_RS25235at 1002.792 kb on - strand, within RR42_RS25235at 1002.792 kb on - strand, within RR42_RS25235at 1003.006 kb on + strand, within RR42_RS25235at 1003.039 kb on - strand, within RR42_RS25235at 1003.099 kb on + strand, within RR42_RS25235at 1003.148 kb on - strand, within RR42_RS25235at 1003.190 kb on + strand, within RR42_RS25235at 1003.225 kb on + strand, within RR42_RS25235at 1003.226 kb on - strand, within RR42_RS25235at 1003.367 kb on + strandat 1003.368 kb on - strandat 1003.368 kb on - strandat 1003.368 kb on - strandat 1003.368 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source 2-Deoxy-D-Ribose 20 mM
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1,000,597 - -1.3
1,000,639 + RR42_RS25225 0.17 +0.2
1,000,640 - RR42_RS25225 0.17 +1.2
1,000,881 + +0.5
1,000,882 - -1.9
1,000,944 + +0.3
1,000,944 + +0.1
1,001,185 + RR42_RS25230 0.19 -0.5
1,001,428 - RR42_RS25230 0.35 +1.1
1,001,652 + RR42_RS25230 0.51 -2.2
1,001,653 - RR42_RS25230 0.51 +2.0
1,001,752 - RR42_RS25230 0.57 +1.4
1,001,752 - RR42_RS25230 0.57 -0.0
1,001,752 - RR42_RS25230 0.57 -0.4
1,002,004 + RR42_RS25230 0.75 -0.7
1,002,005 - RR42_RS25230 0.75 -2.1
1,002,009 + RR42_RS25230 0.75 -0.8
1,002,010 - RR42_RS25230 0.75 -1.2
1,002,010 - RR42_RS25230 0.75 +0.0
1,002,048 - RR42_RS25230 0.78 -1.1
1,002,048 - RR42_RS25230 0.78 -1.4
1,002,081 + RR42_RS25230 0.80 +0.2
1,002,303 + +1.7
1,002,513 + -0.5
1,002,514 - +1.2
1,002,514 - +0.2
1,002,517 + +0.2
1,002,517 + +0.1
1,002,517 + +1.4
1,002,552 - +0.5
1,002,552 - +1.9
1,002,653 + +1.8
1,002,654 - +0.2
1,002,791 + RR42_RS25235 0.10 -1.0
1,002,791 + RR42_RS25235 0.10 +0.2
1,002,791 + RR42_RS25235 0.10 -0.2
1,002,791 + RR42_RS25235 0.10 +0.7
1,002,792 - RR42_RS25235 0.10 +0.1
1,002,792 - RR42_RS25235 0.10 -2.6
1,002,792 - RR42_RS25235 0.10 -1.0
1,003,006 + RR42_RS25235 0.46 -0.6
1,003,039 - RR42_RS25235 0.51 +0.0
1,003,099 + RR42_RS25235 0.61 -0.5
1,003,148 - RR42_RS25235 0.69 -0.2
1,003,190 + RR42_RS25235 0.76 -1.9
1,003,225 + RR42_RS25235 0.82 -0.5
1,003,226 - RR42_RS25235 0.82 -1.0
1,003,367 + -0.4
1,003,368 - -0.3
1,003,368 - -1.0
1,003,368 - -0.8
1,003,368 - -2.4

Or see this region's nucleotide sequence