Experiment: Carbon source 2-Deoxy-D-Ribose 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS22945 and RR42_RS22950 are separated by 177 nucleotides RR42_RS22950 and RR42_RS22955 are separated by 126 nucleotides
RR42_RS22945: RR42_RS22945 - oxidoreductase, at 481,486 to 482,514
_RS22945
RR42_RS22950: RR42_RS22950 - cytochrome C550, at 482,692 to 483,549
_RS22950
RR42_RS22955: RR42_RS22955 - isocitrate dehydrogenase, at 483,676 to 484,707
_RS22955
Position (kb) 482
483
484 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.735 kb on + strand, within RR42_RS22945 at 481.736 kb on - strand, within RR42_RS22945 at 481.810 kb on + strand, within RR42_RS22945 at 481.811 kb on - strand, within RR42_RS22945 at 481.924 kb on + strand, within RR42_RS22945 at 481.924 kb on + strand, within RR42_RS22945 at 481.924 kb on + strand, within RR42_RS22945 at 481.924 kb on + strand, within RR42_RS22945 at 481.925 kb on - strand, within RR42_RS22945 at 481.927 kb on + strand, within RR42_RS22945 at 481.928 kb on - strand, within RR42_RS22945 at 482.205 kb on + strand, within RR42_RS22945 at 482.205 kb on + strand, within RR42_RS22945 at 482.205 kb on + strand, within RR42_RS22945 at 482.206 kb on - strand, within RR42_RS22945 at 482.206 kb on - strand, within RR42_RS22945 at 482.317 kb on + strand, within RR42_RS22945 at 482.400 kb on + strand, within RR42_RS22945 at 482.400 kb on + strand, within RR42_RS22945 at 482.400 kb on + strand, within RR42_RS22945 at 482.426 kb on + strand at 482.427 kb on - strand at 482.591 kb on + strand at 482.592 kb on - strand at 482.646 kb on + strand at 482.647 kb on - strand at 482.679 kb on + strand at 482.680 kb on - strand at 482.710 kb on + strand at 482.772 kb on + strand at 482.773 kb on - strand at 482.773 kb on - strand at 482.791 kb on + strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.792 kb on - strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.809 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.962 kb on + strand, within RR42_RS22950 at 482.963 kb on - strand, within RR42_RS22950 at 482.963 kb on - strand, within RR42_RS22950 at 483.352 kb on - strand, within RR42_RS22950 at 483.352 kb on - strand, within RR42_RS22950 at 483.977 kb on + strand, within RR42_RS22955 at 483.977 kb on + strand, within RR42_RS22955 at 483.977 kb on + strand, within RR42_RS22955 at 484.017 kb on + strand, within RR42_RS22955 at 484.057 kb on + strand, within RR42_RS22955 at 484.058 kb on - strand, within RR42_RS22955 at 484.073 kb on - strand, within RR42_RS22955 at 484.073 kb on - strand, within RR42_RS22955 at 484.075 kb on + strand, within RR42_RS22955 at 484.076 kb on - strand, within RR42_RS22955 at 484.128 kb on + strand, within RR42_RS22955 at 484.171 kb on + strand, within RR42_RS22955 at 484.171 kb on + strand, within RR42_RS22955 at 484.280 kb on - strand, within RR42_RS22955 at 484.371 kb on + strand, within RR42_RS22955
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxy-D-Ribose 20 mM remove 481,735 + RR42_RS22945 0.24 -0.3 481,735 + RR42_RS22945 0.24 +1.4 481,735 + RR42_RS22945 0.24 -2.4 481,735 + RR42_RS22945 0.24 +0.5 481,735 + RR42_RS22945 0.24 +0.8 481,735 + RR42_RS22945 0.24 +0.5 481,736 - RR42_RS22945 0.24 +0.7 481,810 + RR42_RS22945 0.31 -2.4 481,811 - RR42_RS22945 0.32 -0.2 481,924 + RR42_RS22945 0.43 -0.3 481,924 + RR42_RS22945 0.43 -1.6 481,924 + RR42_RS22945 0.43 +0.5 481,924 + RR42_RS22945 0.43 +0.3 481,925 - RR42_RS22945 0.43 -0.3 481,927 + RR42_RS22945 0.43 +2.2 481,928 - RR42_RS22945 0.43 -2.8 482,205 + RR42_RS22945 0.70 +1.4 482,205 + RR42_RS22945 0.70 -0.4 482,205 + RR42_RS22945 0.70 +1.0 482,206 - RR42_RS22945 0.70 -0.2 482,206 - RR42_RS22945 0.70 +0.1 482,317 + RR42_RS22945 0.81 -0.7 482,400 + RR42_RS22945 0.89 -0.9 482,400 + RR42_RS22945 0.89 -0.5 482,400 + RR42_RS22945 0.89 +1.3 482,426 + -1.8 482,427 - -0.0 482,591 + +0.9 482,592 - -0.2 482,646 + +1.0 482,647 - +1.7 482,679 + +0.4 482,680 - +1.6 482,710 + -0.6 482,772 + +1.7 482,773 - +1.9 482,773 - +0.4 482,791 + RR42_RS22950 0.12 +2.9 482,792 - RR42_RS22950 0.12 +1.0 482,792 - RR42_RS22950 0.12 -1.2 482,792 - RR42_RS22950 0.12 +1.8 482,809 + RR42_RS22950 0.14 +1.1 482,809 + RR42_RS22950 0.14 +2.1 482,809 + RR42_RS22950 0.14 -0.2 482,962 + RR42_RS22950 0.31 +0.8 482,962 + RR42_RS22950 0.31 -1.3 482,962 + RR42_RS22950 0.31 +1.4 482,962 + RR42_RS22950 0.31 +1.7 482,963 - RR42_RS22950 0.32 +1.3 482,963 - RR42_RS22950 0.32 +2.0 483,352 - RR42_RS22950 0.77 +1.7 483,352 - RR42_RS22950 0.77 +1.2 483,977 + RR42_RS22955 0.29 +0.1 483,977 + RR42_RS22955 0.29 -2.4 483,977 + RR42_RS22955 0.29 +1.2 484,017 + RR42_RS22955 0.33 +0.4 484,057 + RR42_RS22955 0.37 +0.1 484,058 - RR42_RS22955 0.37 -1.4 484,073 - RR42_RS22955 0.38 -0.5 484,073 - RR42_RS22955 0.38 -0.3 484,075 + RR42_RS22955 0.39 -1.3 484,076 - RR42_RS22955 0.39 -1.3 484,128 + RR42_RS22955 0.44 +0.0 484,171 + RR42_RS22955 0.48 -0.1 484,171 + RR42_RS22955 0.48 +0.2 484,280 - RR42_RS22955 0.59 +0.1 484,371 + RR42_RS22955 0.67 +0.1
Or see this region's nucleotide sequence