Experiment: Uridine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11730 and PGA1_c11740 are separated by 40 nucleotides PGA1_c11740 and PGA1_c11750 are separated by 86 nucleotides PGA1_c11750 and PGA1_c11760 are separated by 135 nucleotides PGA1_c11760 and PGA1_c11770 are separated by 69 nucleotides
PGA1_c11730: PGA1_c11730 - putative glutathione-dependent formaldehyde-activating enzyme, at 1,214,668 to 1,215,084
_c11730
PGA1_c11740: PGA1_c11740 - rhomboid protein-like protein, at 1,215,125 to 1,215,901
_c11740
PGA1_c11750: PGA1_c11750 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 1,215,988 to 1,219,464
_c11750
PGA1_c11760: PGA1_c11760 - putative proline dehydrogenase transcriptional activator, at 1,219,600 to 1,220,073
_c11760
PGA1_c11770: PGA1_c11770 - tetratricopeptide repeat-containing protein, at 1,220,143 to 1,220,775
_c11770
Position (kb)
1215
1216
1217
1218
1219
1220 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1215.041 kb on - strand, within PGA1_c11730 at 1215.041 kb on - strand, within PGA1_c11730 at 1215.063 kb on + strand at 1215.071 kb on - strand at 1215.072 kb on + strand at 1215.073 kb on + strand at 1215.090 kb on - strand at 1215.106 kb on + strand at 1215.106 kb on + strand at 1215.107 kb on + strand at 1215.114 kb on - strand at 1215.114 kb on - strand at 1215.124 kb on - strand at 1215.192 kb on - strand at 1215.192 kb on - strand at 1215.208 kb on + strand, within PGA1_c11740 at 1215.250 kb on - strand, within PGA1_c11740 at 1215.290 kb on - strand, within PGA1_c11740 at 1215.346 kb on + strand, within PGA1_c11740 at 1215.346 kb on + strand, within PGA1_c11740 at 1215.376 kb on + strand, within PGA1_c11740 at 1215.400 kb on - strand, within PGA1_c11740 at 1215.409 kb on + strand, within PGA1_c11740 at 1215.542 kb on - strand, within PGA1_c11740 at 1215.578 kb on + strand, within PGA1_c11740 at 1215.578 kb on + strand, within PGA1_c11740 at 1215.745 kb on - strand, within PGA1_c11740 at 1215.752 kb on - strand, within PGA1_c11740 at 1215.763 kb on - strand, within PGA1_c11740 at 1215.790 kb on + strand, within PGA1_c11740 at 1215.794 kb on + strand, within PGA1_c11740 at 1215.794 kb on + strand, within PGA1_c11740 at 1215.844 kb on + strand at 1215.844 kb on + strand at 1215.847 kb on - strand at 1215.852 kb on - strand at 1215.852 kb on - strand at 1215.856 kb on + strand at 1215.908 kb on + strand at 1215.920 kb on + strand at 1215.950 kb on + strand at 1216.129 kb on + strand at 1216.257 kb on - strand at 1216.264 kb on + strand at 1216.411 kb on + strand, within PGA1_c11750 at 1216.489 kb on - strand, within PGA1_c11750 at 1216.578 kb on + strand, within PGA1_c11750 at 1216.585 kb on - strand, within PGA1_c11750 at 1216.612 kb on + strand, within PGA1_c11750 at 1216.644 kb on + strand, within PGA1_c11750 at 1216.656 kb on + strand, within PGA1_c11750 at 1216.656 kb on + strand, within PGA1_c11750 at 1216.659 kb on - strand, within PGA1_c11750 at 1216.664 kb on - strand, within PGA1_c11750 at 1216.664 kb on - strand, within PGA1_c11750 at 1216.690 kb on - strand, within PGA1_c11750 at 1216.692 kb on + strand, within PGA1_c11750 at 1216.736 kb on + strand, within PGA1_c11750 at 1216.853 kb on + strand, within PGA1_c11750 at 1216.861 kb on - strand, within PGA1_c11750 at 1216.913 kb on + strand, within PGA1_c11750 at 1216.921 kb on + strand, within PGA1_c11750 at 1216.939 kb on + strand, within PGA1_c11750 at 1216.958 kb on + strand, within PGA1_c11750 at 1217.016 kb on + strand, within PGA1_c11750 at 1217.016 kb on + strand, within PGA1_c11750 at 1217.016 kb on + strand, within PGA1_c11750 at 1217.026 kb on + strand, within PGA1_c11750 at 1217.029 kb on - strand, within PGA1_c11750 at 1217.029 kb on - strand, within PGA1_c11750 at 1217.029 kb on - strand, within PGA1_c11750 at 1217.041 kb on - strand, within PGA1_c11750 at 1217.229 kb on + strand, within PGA1_c11750 at 1217.231 kb on - strand, within PGA1_c11750 at 1217.500 kb on + strand, within PGA1_c11750 at 1217.611 kb on + strand, within PGA1_c11750 at 1217.652 kb on + strand, within PGA1_c11750 at 1217.662 kb on + strand, within PGA1_c11750 at 1217.715 kb on - strand, within PGA1_c11750 at 1217.899 kb on - strand, within PGA1_c11750 at 1217.919 kb on + strand, within PGA1_c11750 at 1217.957 kb on + strand, within PGA1_c11750 at 1218.123 kb on + strand, within PGA1_c11750 at 1218.210 kb on + strand, within PGA1_c11750 at 1218.338 kb on + strand, within PGA1_c11750 at 1218.338 kb on + strand, within PGA1_c11750 at 1218.338 kb on + strand, within PGA1_c11750 at 1218.346 kb on - strand, within PGA1_c11750 at 1218.346 kb on - strand, within PGA1_c11750 at 1218.361 kb on - strand, within PGA1_c11750 at 1218.361 kb on - strand, within PGA1_c11750 at 1218.462 kb on + strand, within PGA1_c11750 at 1218.469 kb on + strand, within PGA1_c11750 at 1218.470 kb on - strand, within PGA1_c11750 at 1218.493 kb on + strand, within PGA1_c11750 at 1218.537 kb on + strand, within PGA1_c11750 at 1218.669 kb on + strand, within PGA1_c11750 at 1218.736 kb on + strand, within PGA1_c11750 at 1218.736 kb on + strand, within PGA1_c11750 at 1218.945 kb on - strand, within PGA1_c11750 at 1218.945 kb on - strand, within PGA1_c11750 at 1218.985 kb on + strand, within PGA1_c11750 at 1218.985 kb on + strand, within PGA1_c11750 at 1218.990 kb on + strand, within PGA1_c11750 at 1219.055 kb on + strand, within PGA1_c11750 at 1219.055 kb on + strand, within PGA1_c11750 at 1219.063 kb on - strand, within PGA1_c11750 at 1219.073 kb on - strand, within PGA1_c11750 at 1219.076 kb on + strand, within PGA1_c11750 at 1219.175 kb on + strand at 1219.180 kb on + strand at 1219.180 kb on + strand at 1219.180 kb on + strand at 1219.239 kb on + strand at 1219.395 kb on + strand at 1219.402 kb on + strand at 1219.651 kb on + strand, within PGA1_c11760 at 1219.651 kb on + strand, within PGA1_c11760 at 1219.665 kb on - strand, within PGA1_c11760 at 1219.687 kb on - strand, within PGA1_c11760 at 1219.748 kb on - strand, within PGA1_c11760 at 1219.803 kb on + strand, within PGA1_c11760 at 1219.897 kb on + strand, within PGA1_c11760 at 1219.897 kb on + strand, within PGA1_c11760 at 1219.989 kb on + strand, within PGA1_c11760 at 1220.017 kb on + strand, within PGA1_c11760 at 1220.025 kb on - strand, within PGA1_c11760 at 1220.060 kb on - strand at 1220.070 kb on - strand at 1220.133 kb on + strand at 1220.133 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.159 kb on - strand at 1220.159 kb on - strand at 1220.210 kb on - strand, within PGA1_c11770 at 1220.224 kb on - strand, within PGA1_c11770 at 1220.230 kb on + strand, within PGA1_c11770 at 1220.290 kb on - strand, within PGA1_c11770 at 1220.290 kb on - strand, within PGA1_c11770 at 1220.305 kb on + strand, within PGA1_c11770
Per-strain Table
Position Strand Gene LocusTag Fraction Uridine (N) remove 1,215,041 - PGA1_c11730 0.89 -0.2 1,215,041 - PGA1_c11730 0.89 -0.5 1,215,063 + -0.7 1,215,071 - -1.1 1,215,072 + +1.7 1,215,073 + -1.8 1,215,090 - +0.0 1,215,106 + +0.1 1,215,106 + -0.0 1,215,107 + -0.2 1,215,114 - +0.1 1,215,114 - +1.0 1,215,124 - -0.2 1,215,192 - +1.0 1,215,192 - +0.9 1,215,208 + PGA1_c11740 0.11 -0.8 1,215,250 - PGA1_c11740 0.16 -0.2 1,215,290 - PGA1_c11740 0.21 +0.6 1,215,346 + PGA1_c11740 0.28 +0.3 1,215,346 + PGA1_c11740 0.28 +0.3 1,215,376 + PGA1_c11740 0.32 +0.2 1,215,400 - PGA1_c11740 0.35 +0.6 1,215,409 + PGA1_c11740 0.37 +0.7 1,215,542 - PGA1_c11740 0.54 +0.2 1,215,578 + PGA1_c11740 0.58 -0.2 1,215,578 + PGA1_c11740 0.58 -0.3 1,215,745 - PGA1_c11740 0.80 +0.2 1,215,752 - PGA1_c11740 0.81 -0.1 1,215,763 - PGA1_c11740 0.82 +0.8 1,215,790 + PGA1_c11740 0.86 +0.6 1,215,794 + PGA1_c11740 0.86 -0.4 1,215,794 + PGA1_c11740 0.86 -0.2 1,215,844 + +0.2 1,215,844 + +1.9 1,215,847 - +0.8 1,215,852 - +0.6 1,215,852 - +0.6 1,215,856 + -0.4 1,215,908 + +0.3 1,215,920 + -0.2 1,215,950 + -1.7 1,216,129 + +1.0 1,216,257 - +0.5 1,216,264 + -0.4 1,216,411 + PGA1_c11750 0.12 -0.5 1,216,489 - PGA1_c11750 0.14 +0.0 1,216,578 + PGA1_c11750 0.17 -0.4 1,216,585 - PGA1_c11750 0.17 +0.6 1,216,612 + PGA1_c11750 0.18 -0.7 1,216,644 + PGA1_c11750 0.19 -0.1 1,216,656 + PGA1_c11750 0.19 -0.8 1,216,656 + PGA1_c11750 0.19 +0.5 1,216,659 - PGA1_c11750 0.19 -0.7 1,216,664 - PGA1_c11750 0.19 +0.3 1,216,664 - PGA1_c11750 0.19 +0.1 1,216,690 - PGA1_c11750 0.20 +0.4 1,216,692 + PGA1_c11750 0.20 -0.5 1,216,736 + PGA1_c11750 0.22 -1.0 1,216,853 + PGA1_c11750 0.25 +0.8 1,216,861 - PGA1_c11750 0.25 +0.4 1,216,913 + PGA1_c11750 0.27 +1.0 1,216,921 + PGA1_c11750 0.27 -0.2 1,216,939 + PGA1_c11750 0.27 +1.0 1,216,958 + PGA1_c11750 0.28 -1.0 1,217,016 + PGA1_c11750 0.30 +0.1 1,217,016 + PGA1_c11750 0.30 -0.1 1,217,016 + PGA1_c11750 0.30 +1.0 1,217,026 + PGA1_c11750 0.30 -0.7 1,217,029 - PGA1_c11750 0.30 +0.6 1,217,029 - PGA1_c11750 0.30 -0.2 1,217,029 - PGA1_c11750 0.30 +0.5 1,217,041 - PGA1_c11750 0.30 -1.2 1,217,229 + PGA1_c11750 0.36 +0.3 1,217,231 - PGA1_c11750 0.36 +0.6 1,217,500 + PGA1_c11750 0.43 +0.4 1,217,611 + PGA1_c11750 0.47 +0.4 1,217,652 + PGA1_c11750 0.48 +1.1 1,217,662 + PGA1_c11750 0.48 +1.0 1,217,715 - PGA1_c11750 0.50 +0.5 1,217,899 - PGA1_c11750 0.55 +0.1 1,217,919 + PGA1_c11750 0.56 -0.1 1,217,957 + PGA1_c11750 0.57 +0.5 1,218,123 + PGA1_c11750 0.61 +0.2 1,218,210 + PGA1_c11750 0.64 +0.2 1,218,338 + PGA1_c11750 0.68 +0.1 1,218,338 + PGA1_c11750 0.68 -1.5 1,218,338 + PGA1_c11750 0.68 +0.8 1,218,346 - PGA1_c11750 0.68 +1.0 1,218,346 - PGA1_c11750 0.68 +1.3 1,218,361 - PGA1_c11750 0.68 +0.8 1,218,361 - PGA1_c11750 0.68 +0.2 1,218,462 + PGA1_c11750 0.71 -2.0 1,218,469 + PGA1_c11750 0.71 -0.2 1,218,470 - PGA1_c11750 0.71 +0.3 1,218,493 + PGA1_c11750 0.72 +1.0 1,218,537 + PGA1_c11750 0.73 +0.4 1,218,669 + PGA1_c11750 0.77 -0.0 1,218,736 + PGA1_c11750 0.79 -0.0 1,218,736 + PGA1_c11750 0.79 +0.1 1,218,945 - PGA1_c11750 0.85 +0.9 1,218,945 - PGA1_c11750 0.85 +0.3 1,218,985 + PGA1_c11750 0.86 +0.7 1,218,985 + PGA1_c11750 0.86 +0.2 1,218,990 + PGA1_c11750 0.86 +2.1 1,219,055 + PGA1_c11750 0.88 +1.7 1,219,055 + PGA1_c11750 0.88 -0.5 1,219,063 - PGA1_c11750 0.88 +0.0 1,219,073 - PGA1_c11750 0.89 -0.3 1,219,076 + PGA1_c11750 0.89 +0.9 1,219,175 + -0.4 1,219,180 + -0.0 1,219,180 + -0.0 1,219,180 + -0.2 1,219,239 + +0.3 1,219,395 + +0.8 1,219,402 + -0.5 1,219,651 + PGA1_c11760 0.11 -1.0 1,219,651 + PGA1_c11760 0.11 -0.2 1,219,665 - PGA1_c11760 0.14 +1.0 1,219,687 - PGA1_c11760 0.18 -0.1 1,219,748 - PGA1_c11760 0.31 +0.1 1,219,803 + PGA1_c11760 0.43 -0.1 1,219,897 + PGA1_c11760 0.63 +0.0 1,219,897 + PGA1_c11760 0.63 -0.8 1,219,989 + PGA1_c11760 0.82 -0.5 1,220,017 + PGA1_c11760 0.88 -0.7 1,220,025 - PGA1_c11760 0.90 +0.6 1,220,060 - -0.4 1,220,070 - -0.3 1,220,133 + +0.1 1,220,133 + +0.8 1,220,151 + +0.0 1,220,151 + +0.6 1,220,151 + +0.5 1,220,151 + -1.5 1,220,159 - +0.0 1,220,159 - -0.8 1,220,210 - PGA1_c11770 0.11 +0.2 1,220,224 - PGA1_c11770 0.13 +0.3 1,220,230 + PGA1_c11770 0.14 -1.1 1,220,290 - PGA1_c11770 0.23 +0.7 1,220,290 - PGA1_c11770 0.23 -0.2 1,220,305 + PGA1_c11770 0.26 +0.5
Or see this region's nucleotide sequence