Experiment: SBM - no RNA protect
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO0304 and SO0305 are separated by 393 nucleotides SO0305 and SO0306 are separated by 316 nucleotides SO0306 and SO0308 are separated by 300 nucleotides
SO0304: SO0304 - hypothetical endonuclease (NCBI ptt file), at 309,390 to 310,352
SO0304
SO0305: SO0305 - hypothetical protein (NCBI ptt file), at 310,746 to 310,859
SO0305
SO0306: SO0306 - hypothetical protein (NCBI ptt file), at 311,176 to 312,687
SO0306
SO0308: SO0308 - conserved hypothetical protein (NCBI ptt file), at 312,988 to 314,208
SO0308
Position (kb)
311
312
313 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 310.281 kb on - strand at 310.367 kb on - strand at 310.388 kb on - strand at 310.506 kb on + strand at 310.527 kb on + strand at 310.544 kb on + strand at 310.552 kb on - strand at 310.552 kb on - strand at 310.552 kb on - strand at 310.552 kb on - strand at 310.552 kb on - strand at 310.564 kb on + strand at 310.597 kb on - strand at 310.617 kb on + strand at 310.649 kb on + strand at 310.657 kb on - strand at 310.707 kb on + strand at 310.707 kb on + strand at 310.707 kb on + strand at 310.707 kb on + strand at 310.707 kb on + strand at 310.707 kb on + strand at 310.715 kb on - strand at 310.770 kb on - strand, within SO0305 at 310.820 kb on - strand, within SO0305 at 310.917 kb on + strand at 310.930 kb on + strand at 310.962 kb on + strand at 311.086 kb on - strand at 311.153 kb on - strand at 311.234 kb on + strand at 311.242 kb on - strand at 311.242 kb on - strand at 311.268 kb on + strand at 311.269 kb on + strand at 311.296 kb on - strand at 311.502 kb on - strand, within SO0306 at 311.520 kb on + strand, within SO0306 at 311.574 kb on - strand, within SO0306 at 311.648 kb on + strand, within SO0306 at 311.741 kb on + strand, within SO0306 at 311.807 kb on + strand, within SO0306 at 311.807 kb on + strand, within SO0306 at 311.807 kb on + strand, within SO0306 at 311.838 kb on - strand, within SO0306 at 311.843 kb on + strand, within SO0306 at 311.843 kb on - strand, within SO0306 at 311.886 kb on + strand, within SO0306 at 311.893 kb on - strand, within SO0306 at 311.904 kb on - strand, within SO0306 at 311.975 kb on + strand, within SO0306 at 311.989 kb on + strand, within SO0306 at 312.033 kb on + strand, within SO0306 at 312.041 kb on - strand, within SO0306 at 312.041 kb on - strand, within SO0306 at 312.041 kb on - strand, within SO0306 at 312.090 kb on + strand, within SO0306 at 312.100 kb on - strand, within SO0306 at 312.125 kb on + strand, within SO0306 at 312.304 kb on + strand, within SO0306 at 312.339 kb on - strand, within SO0306 at 312.365 kb on + strand, within SO0306 at 312.437 kb on + strand, within SO0306 at 312.445 kb on - strand, within SO0306 at 312.445 kb on - strand, within SO0306 at 312.457 kb on - strand, within SO0306 at 312.512 kb on - strand, within SO0306 at 312.514 kb on + strand, within SO0306 at 312.540 kb on + strand at 312.626 kb on - strand at 312.751 kb on + strand at 312.798 kb on - strand at 312.845 kb on + strand at 312.845 kb on + strand at 312.845 kb on + strand at 312.845 kb on + strand at 312.845 kb on + strand at 312.853 kb on - strand at 312.853 kb on - strand at 312.853 kb on - strand at 312.853 kb on - strand at 312.853 kb on - strand at 312.972 kb on + strand at 312.980 kb on - strand at 312.980 kb on - strand at 313.001 kb on - strand at 313.027 kb on + strand at 313.074 kb on + strand at 313.086 kb on + strand at 313.162 kb on + strand, within SO0308 at 313.279 kb on + strand, within SO0308 at 313.302 kb on + strand, within SO0308 at 313.322 kb on + strand, within SO0308 at 313.334 kb on + strand, within SO0308 at 313.360 kb on + strand, within SO0308 at 313.360 kb on + strand, within SO0308 at 313.368 kb on - strand, within SO0308 at 313.457 kb on + strand, within SO0308 at 313.468 kb on + strand, within SO0308 at 313.468 kb on + strand, within SO0308 at 313.476 kb on - strand, within SO0308 at 313.476 kb on - strand, within SO0308 at 313.522 kb on - strand, within SO0308 at 313.567 kb on + strand, within SO0308 at 313.573 kb on + strand, within SO0308 at 313.603 kb on - strand, within SO0308 at 313.671 kb on - strand, within SO0308
Per-strain Table
Position Strand Gene LocusTag Fraction SBM - no RNA protect remove 310,281 - +0.1 310,367 - +0.4 310,388 - +0.1 310,506 + -1.0 310,527 + -0.3 310,544 + +0.2 310,552 - +0.6 310,552 - +1.3 310,552 - -0.6 310,552 - +1.5 310,552 - -0.5 310,564 + +0.7 310,597 - +0.5 310,617 + -1.7 310,649 + +0.4 310,657 - -0.7 310,707 + -0.5 310,707 + +0.3 310,707 + +0.6 310,707 + +0.5 310,707 + +0.8 310,707 + +0.5 310,715 - -0.4 310,770 - SO0305 0.21 +0.1 310,820 - SO0305 0.65 +0.4 310,917 + +0.1 310,930 + -0.5 310,962 + -0.5 311,086 - +0.0 311,153 - -1.3 311,234 + -0.1 311,242 - -0.4 311,242 - +0.4 311,268 + +0.0 311,269 + +1.9 311,296 - +0.6 311,502 - SO0306 0.22 -0.2 311,520 + SO0306 0.23 +1.3 311,574 - SO0306 0.26 +0.3 311,648 + SO0306 0.31 +0.1 311,741 + SO0306 0.37 +1.1 311,807 + SO0306 0.42 +0.6 311,807 + SO0306 0.42 +0.8 311,807 + SO0306 0.42 +0.9 311,838 - SO0306 0.44 +0.1 311,843 + SO0306 0.44 +1.2 311,843 - SO0306 0.44 +0.1 311,886 + SO0306 0.47 +0.6 311,893 - SO0306 0.47 +0.5 311,904 - SO0306 0.48 +1.0 311,975 + SO0306 0.53 +0.2 311,989 + SO0306 0.54 +1.0 312,033 + SO0306 0.57 +0.5 312,041 - SO0306 0.57 -0.2 312,041 - SO0306 0.57 +0.7 312,041 - SO0306 0.57 +0.0 312,090 + SO0306 0.60 +0.7 312,100 - SO0306 0.61 +1.4 312,125 + SO0306 0.63 -0.2 312,304 + SO0306 0.75 +0.1 312,339 - SO0306 0.77 +0.5 312,365 + SO0306 0.79 +0.3 312,437 + SO0306 0.83 +1.0 312,445 - SO0306 0.84 +1.5 312,445 - SO0306 0.84 +2.5 312,457 - SO0306 0.85 +1.2 312,512 - SO0306 0.88 +0.4 312,514 + SO0306 0.88 -1.9 312,540 + +2.0 312,626 - +1.0 312,751 + -0.1 312,798 - +1.4 312,845 + -0.1 312,845 + -0.3 312,845 + +0.4 312,845 + +0.6 312,845 + +0.3 312,853 - +0.5 312,853 - +0.1 312,853 - -0.5 312,853 - +0.7 312,853 - +0.0 312,972 + +0.7 312,980 - -1.4 312,980 - -0.5 313,001 - -0.3 313,027 + +0.5 313,074 + +0.1 313,086 + -0.2 313,162 + SO0308 0.14 -0.3 313,279 + SO0308 0.24 +0.1 313,302 + SO0308 0.26 +0.3 313,322 + SO0308 0.27 -1.9 313,334 + SO0308 0.28 +0.9 313,360 + SO0308 0.30 -0.8 313,360 + SO0308 0.30 +1.5 313,368 - SO0308 0.31 +1.5 313,457 + SO0308 0.38 +0.4 313,468 + SO0308 0.39 -0.8 313,468 + SO0308 0.39 -0.2 313,476 - SO0308 0.40 +0.9 313,476 - SO0308 0.40 -0.3 313,522 - SO0308 0.44 +0.1 313,567 + SO0308 0.47 -0.3 313,573 + SO0308 0.48 -0.4 313,603 - SO0308 0.50 -0.2 313,671 - SO0308 0.56 +0.2
Or see this region's nucleotide sequence