Experiment: Carbon source Deoxyribonucleic from herring sperm 5 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30160 and RR42_RS30165 are separated by 178 nucleotides RR42_RS30165 and RR42_RS30170 are separated by 123 nucleotides
RR42_RS30160: RR42_RS30160 - sulfatase, at 2,140,146 to 2,141,345
_RS30160
RR42_RS30165: RR42_RS30165 - membrane protein, at 2,141,524 to 2,142,720
_RS30165
RR42_RS30170: RR42_RS30170 - aldolase, at 2,142,844 to 2,143,608
_RS30170
Position (kb)
2141
2142
2143 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.666 kb on + strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.667 kb on - strand, within RR42_RS30160 at 2140.748 kb on - strand, within RR42_RS30160 at 2140.871 kb on - strand, within RR42_RS30160 at 2141.014 kb on + strand, within RR42_RS30160 at 2141.014 kb on + strand, within RR42_RS30160 at 2141.015 kb on - strand, within RR42_RS30160 at 2141.454 kb on - strand at 2141.454 kb on - strand at 2141.507 kb on + strand at 2141.508 kb on - strand at 2141.508 kb on - strand at 2141.526 kb on - strand at 2141.581 kb on + strand at 2141.582 kb on - strand at 2141.645 kb on + strand, within RR42_RS30165 at 2141.646 kb on - strand, within RR42_RS30165 at 2141.646 kb on - strand, within RR42_RS30165 at 2141.660 kb on + strand, within RR42_RS30165 at 2141.660 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.774 kb on + strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.775 kb on - strand, within RR42_RS30165 at 2141.778 kb on - strand, within RR42_RS30165 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.924 kb on + strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2141.925 kb on - strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.029 kb on + strand, within RR42_RS30165 at 2142.030 kb on - strand, within RR42_RS30165 at 2142.054 kb on + strand, within RR42_RS30165 at 2142.055 kb on - strand, within RR42_RS30165 at 2142.143 kb on + strand, within RR42_RS30165 at 2142.144 kb on - strand, within RR42_RS30165 at 2142.182 kb on + strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.294 kb on - strand, within RR42_RS30165 at 2142.512 kb on - strand, within RR42_RS30165 at 2142.626 kb on + strand at 2142.626 kb on + strand at 2142.626 kb on + strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.627 kb on - strand at 2142.705 kb on - strand at 2142.705 kb on - strand at 2142.705 kb on - strand at 2142.709 kb on - strand at 2142.709 kb on - strand at 2142.709 kb on - strand at 2142.730 kb on + strand at 2142.730 kb on + strand at 2142.733 kb on + strand at 2142.734 kb on - strand at 2142.734 kb on - strand at 2142.845 kb on + strand at 2142.846 kb on - strand at 2142.846 kb on - strand at 2143.034 kb on + strand, within RR42_RS30170 at 2143.034 kb on + strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.035 kb on - strand, within RR42_RS30170 at 2143.266 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.272 kb on + strand, within RR42_RS30170 at 2143.600 kb on - strand at 2143.630 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Deoxyribonucleic from herring sperm 5 mg/mL remove 2,140,666 + RR42_RS30160 0.43 -2.0 2,140,666 + RR42_RS30160 0.43 -2.7 2,140,666 + RR42_RS30160 0.43 -0.4 2,140,666 + RR42_RS30160 0.43 -0.2 2,140,667 - RR42_RS30160 0.43 +1.3 2,140,667 - RR42_RS30160 0.43 -3.0 2,140,667 - RR42_RS30160 0.43 -1.3 2,140,748 - RR42_RS30160 0.50 -0.1 2,140,871 - RR42_RS30160 0.60 -0.8 2,141,014 + RR42_RS30160 0.72 +1.4 2,141,014 + RR42_RS30160 0.72 -0.0 2,141,015 - RR42_RS30160 0.72 -0.3 2,141,454 - -0.8 2,141,454 - -0.4 2,141,507 + -2.0 2,141,508 - -1.2 2,141,508 - -2.4 2,141,526 - -1.5 2,141,581 + -0.2 2,141,582 - -2.2 2,141,645 + RR42_RS30165 0.10 +1.0 2,141,646 - RR42_RS30165 0.10 -3.6 2,141,646 - RR42_RS30165 0.10 +0.2 2,141,660 + RR42_RS30165 0.11 -0.1 2,141,660 + RR42_RS30165 0.11 +0.2 2,141,774 + RR42_RS30165 0.21 -0.2 2,141,774 + RR42_RS30165 0.21 -0.9 2,141,774 + RR42_RS30165 0.21 +1.0 2,141,775 - RR42_RS30165 0.21 -0.1 2,141,775 - RR42_RS30165 0.21 +1.7 2,141,775 - RR42_RS30165 0.21 -0.7 2,141,775 - RR42_RS30165 0.21 -0.1 2,141,778 - RR42_RS30165 0.21 +1.0 2,141,924 + RR42_RS30165 0.33 -0.7 2,141,924 + RR42_RS30165 0.33 +0.5 2,141,925 - RR42_RS30165 0.34 +1.0 2,141,925 - RR42_RS30165 0.34 -1.1 2,142,029 + RR42_RS30165 0.42 +0.7 2,142,029 + RR42_RS30165 0.42 +0.9 2,142,030 - RR42_RS30165 0.42 +0.7 2,142,054 + RR42_RS30165 0.44 +1.1 2,142,055 - RR42_RS30165 0.44 -0.3 2,142,143 + RR42_RS30165 0.52 +1.1 2,142,144 - RR42_RS30165 0.52 -1.0 2,142,182 + RR42_RS30165 0.55 -0.4 2,142,294 - RR42_RS30165 0.64 +0.4 2,142,294 - RR42_RS30165 0.64 +0.9 2,142,294 - RR42_RS30165 0.64 -2.8 2,142,294 - RR42_RS30165 0.64 +1.4 2,142,512 - RR42_RS30165 0.83 +1.3 2,142,626 + -1.5 2,142,626 + +0.1 2,142,626 + +1.3 2,142,627 - -3.6 2,142,627 - -1.7 2,142,627 - +0.8 2,142,627 - -0.5 2,142,705 - -0.4 2,142,705 - +0.0 2,142,705 - +0.4 2,142,709 - -1.6 2,142,709 - -3.1 2,142,709 - +0.4 2,142,730 + +0.6 2,142,730 + +1.8 2,142,733 + -2.6 2,142,734 - +0.4 2,142,734 - +1.1 2,142,845 + -1.8 2,142,846 - -1.7 2,142,846 - -0.2 2,143,034 + RR42_RS30170 0.25 +0.1 2,143,034 + RR42_RS30170 0.25 +1.0 2,143,035 - RR42_RS30170 0.25 +0.8 2,143,035 - RR42_RS30170 0.25 -0.3 2,143,035 - RR42_RS30170 0.25 -0.2 2,143,266 + RR42_RS30170 0.55 +0.5 2,143,272 + RR42_RS30170 0.56 -1.8 2,143,272 + RR42_RS30170 0.56 -1.0 2,143,600 - -1.0 2,143,630 + -1.4
Or see this region's nucleotide sequence