Experiment: Carbon source Deoxyribonucleic from herring sperm 5 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29035 and RR42_RS29040 are separated by 346 nucleotides RR42_RS29040 and RR42_RS29045 are separated by 177 nucleotides RR42_RS29045 and RR42_RS29050 are separated by 179 nucleotides
RR42_RS29035: RR42_RS29035 - acyl-CoA synthetase, at 1,868,823 to 1,870,505
_RS29035
RR42_RS29040: RR42_RS29040 - histidyl-tRNA synthetase, at 1,870,852 to 1,871,316
_RS29040
RR42_RS29045: RR42_RS29045 - esterase, at 1,871,494 to 1,872,456
_RS29045
RR42_RS29050: RR42_RS29050 - glutamate synthase, at 1,872,636 to 1,874,225
_RS29050
Position (kb)
1871
1872
1873 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.599 kb on + strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.600 kb on - strand at 1870.601 kb on + strand at 1870.602 kb on - strand at 1870.602 kb on - strand at 1870.602 kb on - strand at 1870.603 kb on + strand at 1870.604 kb on - strand at 1870.841 kb on + strand at 1870.841 kb on + strand at 1870.842 kb on - strand at 1870.842 kb on - strand at 1870.845 kb on + strand at 1870.846 kb on - strand at 1870.846 kb on - strand at 1870.852 kb on - strand at 1870.934 kb on + strand, within RR42_RS29040 at 1871.048 kb on + strand, within RR42_RS29040 at 1871.186 kb on + strand, within RR42_RS29040 at 1871.186 kb on + strand, within RR42_RS29040 at 1871.187 kb on - strand, within RR42_RS29040 at 1871.187 kb on - strand, within RR42_RS29040 at 1871.432 kb on + strand at 1871.500 kb on - strand at 1871.684 kb on - strand, within RR42_RS29045 at 1871.684 kb on - strand, within RR42_RS29045 at 1871.728 kb on + strand, within RR42_RS29045 at 1871.729 kb on - strand, within RR42_RS29045 at 1871.830 kb on + strand, within RR42_RS29045 at 1871.887 kb on + strand, within RR42_RS29045 at 1872.127 kb on + strand, within RR42_RS29045 at 1872.128 kb on - strand, within RR42_RS29045 at 1872.172 kb on + strand, within RR42_RS29045 at 1872.172 kb on + strand, within RR42_RS29045 at 1872.172 kb on + strand, within RR42_RS29045 at 1872.173 kb on - strand, within RR42_RS29045 at 1872.173 kb on - strand, within RR42_RS29045 at 1872.173 kb on - strand, within RR42_RS29045 at 1872.173 kb on - strand, within RR42_RS29045 at 1872.251 kb on - strand, within RR42_RS29045 at 1872.289 kb on + strand, within RR42_RS29045 at 1872.289 kb on + strand, within RR42_RS29045 at 1872.293 kb on - strand, within RR42_RS29045 at 1872.293 kb on - strand, within RR42_RS29045 at 1872.299 kb on - strand, within RR42_RS29045 at 1872.299 kb on - strand, within RR42_RS29045 at 1872.454 kb on + strand at 1872.455 kb on - strand at 1872.455 kb on - strand at 1872.501 kb on + strand at 1872.502 kb on - strand at 1872.506 kb on + strand at 1872.507 kb on - strand at 1872.507 kb on - strand at 1872.507 kb on - strand at 1872.507 kb on - strand at 1872.625 kb on + strand at 1872.651 kb on + strand at 1872.868 kb on - strand, within RR42_RS29050 at 1872.868 kb on - strand, within RR42_RS29050 at 1872.927 kb on + strand, within RR42_RS29050 at 1872.928 kb on - strand, within RR42_RS29050 at 1872.928 kb on - strand, within RR42_RS29050 at 1873.054 kb on + strand, within RR42_RS29050 at 1873.055 kb on - strand, within RR42_RS29050 at 1873.083 kb on + strand, within RR42_RS29050 at 1873.083 kb on + strand, within RR42_RS29050 at 1873.182 kb on - strand, within RR42_RS29050 at 1873.215 kb on + strand, within RR42_RS29050 at 1873.216 kb on - strand, within RR42_RS29050 at 1873.436 kb on + strand, within RR42_RS29050
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Deoxyribonucleic from herring sperm 5 mg/mL remove 1,870,599 + +2.5 1,870,599 + -1.2 1,870,599 + -0.6 1,870,599 + -0.1 1,870,599 + +1.7 1,870,599 + +1.8 1,870,600 - -0.6 1,870,600 - -1.4 1,870,600 - +0.4 1,870,600 - -2.9 1,870,600 - +1.0 1,870,601 + -0.5 1,870,602 - -0.4 1,870,602 - -0.7 1,870,602 - +1.5 1,870,603 + +0.2 1,870,604 - -0.6 1,870,841 + -2.9 1,870,841 + -2.0 1,870,842 - +1.9 1,870,842 - -0.9 1,870,845 + -0.6 1,870,846 - +0.8 1,870,846 - -0.8 1,870,852 - -3.0 1,870,934 + RR42_RS29040 0.18 -0.7 1,871,048 + RR42_RS29040 0.42 -1.7 1,871,186 + RR42_RS29040 0.72 -1.4 1,871,186 + RR42_RS29040 0.72 +1.0 1,871,187 - RR42_RS29040 0.72 -0.1 1,871,187 - RR42_RS29040 0.72 -0.4 1,871,432 + +1.2 1,871,500 - +0.1 1,871,684 - RR42_RS29045 0.20 -1.1 1,871,684 - RR42_RS29045 0.20 -0.5 1,871,728 + RR42_RS29045 0.24 +0.5 1,871,729 - RR42_RS29045 0.24 +0.7 1,871,830 + RR42_RS29045 0.35 +0.9 1,871,887 + RR42_RS29045 0.41 +0.9 1,872,127 + RR42_RS29045 0.66 +0.4 1,872,128 - RR42_RS29045 0.66 -1.9 1,872,172 + RR42_RS29045 0.70 -0.3 1,872,172 + RR42_RS29045 0.70 -3.0 1,872,172 + RR42_RS29045 0.70 -0.1 1,872,173 - RR42_RS29045 0.71 -1.4 1,872,173 - RR42_RS29045 0.71 -2.5 1,872,173 - RR42_RS29045 0.71 +0.2 1,872,173 - RR42_RS29045 0.71 +0.9 1,872,251 - RR42_RS29045 0.79 +0.3 1,872,289 + RR42_RS29045 0.83 +0.2 1,872,289 + RR42_RS29045 0.83 +0.9 1,872,293 - RR42_RS29045 0.83 -0.2 1,872,293 - RR42_RS29045 0.83 +0.6 1,872,299 - RR42_RS29045 0.84 +0.9 1,872,299 - RR42_RS29045 0.84 -1.1 1,872,454 + +1.4 1,872,455 - -1.6 1,872,455 - -0.6 1,872,501 + +2.2 1,872,502 - +1.0 1,872,506 + +0.5 1,872,507 - +0.2 1,872,507 - -1.1 1,872,507 - -0.5 1,872,507 - -1.4 1,872,625 + +0.1 1,872,651 + -1.7 1,872,868 - RR42_RS29050 0.15 -1.1 1,872,868 - RR42_RS29050 0.15 -1.2 1,872,927 + RR42_RS29050 0.18 +0.4 1,872,928 - RR42_RS29050 0.18 +0.4 1,872,928 - RR42_RS29050 0.18 +1.2 1,873,054 + RR42_RS29050 0.26 +0.5 1,873,055 - RR42_RS29050 0.26 -2.4 1,873,083 + RR42_RS29050 0.28 -3.0 1,873,083 + RR42_RS29050 0.28 -0.8 1,873,182 - RR42_RS29050 0.34 -1.0 1,873,215 + RR42_RS29050 0.36 -2.2 1,873,216 - RR42_RS29050 0.36 +0.5 1,873,436 + RR42_RS29050 0.50 +0.4
Or see this region's nucleotide sequence