Experiment: Carbon source Deoxyribonucleic from herring sperm 5 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00950 and RR42_RS00955 are separated by 118 nucleotides RR42_RS00955 and RR42_RS00960 are separated by 79 nucleotides RR42_RS00960 and RR42_RS00965 are separated by 60 nucleotides RR42_RS00965 and RR42_RS00970 are separated by 12 nucleotides
RR42_RS00950: RR42_RS00950 - glyoxylate reductase, at 226,613 to 227,554
_RS00950
RR42_RS00955: RR42_RS00955 - hydroxymethylglutaryl-CoA lyase, at 227,673 to 228,608
_RS00955
RR42_RS00960: RR42_RS00960 - prolyl-tRNA synthetase, at 228,688 to 229,167
_RS00960
RR42_RS00965: RR42_RS00965 - Fe-S oxidoreductase, at 229,228 to 229,497
_RS00965
RR42_RS00970: RR42_RS00970 - DSBA oxidoreductase, at 229,510 to 230,199
_RS00970
Position (kb)
227
228
229 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 226.733 kb on + strand, within RR42_RS00950 at 226.734 kb on - strand, within RR42_RS00950 at 226.919 kb on + strand, within RR42_RS00950 at 226.964 kb on + strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 226.965 kb on - strand, within RR42_RS00950 at 227.276 kb on + strand, within RR42_RS00950 at 227.277 kb on - strand, within RR42_RS00950 at 227.440 kb on + strand, within RR42_RS00950 at 227.441 kb on - strand, within RR42_RS00950 at 227.614 kb on + strand at 227.615 kb on - strand at 227.693 kb on - strand at 227.805 kb on - strand, within RR42_RS00955 at 227.901 kb on + strand, within RR42_RS00955 at 227.921 kb on + strand, within RR42_RS00955 at 227.922 kb on - strand, within RR42_RS00955 at 227.922 kb on - strand, within RR42_RS00955 at 228.158 kb on - strand, within RR42_RS00955 at 228.158 kb on - strand, within RR42_RS00955 at 228.307 kb on - strand, within RR42_RS00955 at 228.307 kb on - strand, within RR42_RS00955 at 228.441 kb on + strand, within RR42_RS00955 at 228.441 kb on + strand, within RR42_RS00955 at 228.442 kb on - strand, within RR42_RS00955 at 228.442 kb on - strand, within RR42_RS00955 at 228.604 kb on + strand at 228.604 kb on + strand at 228.604 kb on + strand at 228.605 kb on - strand at 228.606 kb on + strand at 228.606 kb on + strand at 228.607 kb on - strand at 228.607 kb on - strand at 228.634 kb on - strand at 228.634 kb on - strand at 228.667 kb on - strand at 228.996 kb on + strand, within RR42_RS00960 at 228.996 kb on + strand, within RR42_RS00960 at 229.181 kb on - strand at 229.181 kb on - strand at 229.181 kb on - strand at 229.345 kb on + strand, within RR42_RS00965 at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.495 kb on + strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.496 kb on - strand at 229.531 kb on + strand at 229.532 kb on - strand at 229.558 kb on + strand at 229.559 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Deoxyribonucleic from herring sperm 5 mg/mL remove 226,733 + RR42_RS00950 0.13 -0.3 226,734 - RR42_RS00950 0.13 -0.2 226,919 + RR42_RS00950 0.32 -1.5 226,964 + RR42_RS00950 0.37 +0.6 226,965 - RR42_RS00950 0.37 +1.4 226,965 - RR42_RS00950 0.37 -0.4 226,965 - RR42_RS00950 0.37 +1.9 227,276 + RR42_RS00950 0.70 +0.8 227,277 - RR42_RS00950 0.70 +2.4 227,440 + RR42_RS00950 0.88 +0.0 227,441 - RR42_RS00950 0.88 +1.1 227,614 + +0.2 227,615 - +3.3 227,693 - +3.8 227,805 - RR42_RS00955 0.14 +0.7 227,901 + RR42_RS00955 0.24 +3.5 227,921 + RR42_RS00955 0.26 +2.6 227,922 - RR42_RS00955 0.27 +2.2 227,922 - RR42_RS00955 0.27 +2.4 228,158 - RR42_RS00955 0.52 +3.1 228,158 - RR42_RS00955 0.52 +3.3 228,307 - RR42_RS00955 0.68 +2.4 228,307 - RR42_RS00955 0.68 +2.8 228,441 + RR42_RS00955 0.82 +4.2 228,441 + RR42_RS00955 0.82 +3.7 228,442 - RR42_RS00955 0.82 +4.0 228,442 - RR42_RS00955 0.82 +3.3 228,604 + +0.0 228,604 + -3.2 228,604 + -0.6 228,605 - +0.4 228,606 + +0.2 228,606 + +0.6 228,607 - +1.0 228,607 - +2.1 228,634 - +0.2 228,634 - -0.9 228,667 - -0.1 228,996 + RR42_RS00960 0.64 +1.0 228,996 + RR42_RS00960 0.64 -0.1 229,181 - -0.1 229,181 - -2.3 229,181 - +0.5 229,345 + RR42_RS00965 0.43 -1.2 229,495 + +0.3 229,495 + +1.0 229,495 + +2.8 229,495 + -0.2 229,496 - -1.3 229,496 - -1.2 229,496 - -1.0 229,496 - -3.0 229,496 - -0.3 229,496 - -0.6 229,531 + -0.2 229,532 - +1.8 229,558 + +0.9 229,559 - -0.3
Or see this region's nucleotide sequence