Experiment: BG11 with Chloride 15.625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbC and Synpcc7942_0059 are separated by 20 nucleotides Synpcc7942_0059 and Synpcc7942_0060 overlap by 22 nucleotides Synpcc7942_0060 and rfbG overlap by 4 nucleotides rfbG and rfbF overlap by 4 nucleotides
Synpcc7942_0058: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 60,301 to 60,846
rfbC
Synpcc7942_0059: Synpcc7942_0059 - hypothetical protein, at 60,867 to 61,112
_0059
Synpcc7942_0060: Synpcc7942_0060 - hypothetical protein, at 61,091 to 61,369
_0060
Synpcc7942_0061: rfbG - CDP-glucose 4,6-dehydratase, at 61,366 to 62,472
rfbG
Synpcc7942_0062: rfbF - glucose-1-phosphate cytidylyltransferase, at 62,469 to 63,242
rfbF
Position (kb) 61
62
63 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 60.378 kb on + strand, within rfbC at 60.386 kb on - strand, within rfbC at 60.396 kb on - strand, within rfbC at 60.436 kb on - strand, within rfbC at 60.468 kb on + strand, within rfbC at 60.476 kb on - strand, within rfbC at 60.496 kb on + strand, within rfbC at 60.506 kb on + strand, within rfbC at 60.512 kb on - strand, within rfbC at 60.514 kb on - strand, within rfbC at 60.514 kb on - strand, within rfbC at 60.514 kb on - strand, within rfbC at 60.516 kb on + strand, within rfbC at 60.555 kb on - strand, within rfbC at 60.573 kb on + strand, within rfbC at 60.587 kb on + strand, within rfbC at 60.587 kb on + strand, within rfbC at 60.589 kb on - strand, within rfbC at 60.595 kb on - strand, within rfbC at 60.595 kb on - strand, within rfbC at 60.621 kb on + strand, within rfbC at 60.629 kb on - strand, within rfbC at 60.666 kb on + strand, within rfbC at 60.682 kb on - strand, within rfbC at 60.688 kb on - strand, within rfbC at 60.696 kb on - strand, within rfbC at 60.704 kb on + strand, within rfbC at 60.712 kb on - strand, within rfbC at 60.716 kb on + strand, within rfbC at 60.724 kb on - strand, within rfbC at 60.724 kb on - strand, within rfbC at 60.724 kb on - strand, within rfbC at 60.813 kb on - strand at 60.818 kb on - strand at 60.835 kb on - strand at 60.953 kb on + strand, within Synpcc7942_0059 at 60.961 kb on - strand, within Synpcc7942_0059 at 60.975 kb on - strand, within Synpcc7942_0059 at 60.996 kb on - strand, within Synpcc7942_0059 at 61.050 kb on - strand, within Synpcc7942_0059 at 61.058 kb on - strand, within Synpcc7942_0059 at 61.083 kb on - strand, within Synpcc7942_0059 at 61.119 kb on + strand, within Synpcc7942_0060 at 61.208 kb on - strand, within Synpcc7942_0060 at 61.225 kb on + strand, within Synpcc7942_0060 at 61.270 kb on - strand, within Synpcc7942_0060 at 61.282 kb on + strand, within Synpcc7942_0060 at 61.290 kb on - strand, within Synpcc7942_0060 at 61.295 kb on - strand, within Synpcc7942_0060 at 61.317 kb on + strand, within Synpcc7942_0060 at 61.361 kb on - strand at 61.362 kb on - strand at 61.371 kb on - strand at 61.387 kb on + strand at 61.388 kb on - strand at 61.389 kb on + strand at 61.395 kb on - strand at 61.400 kb on + strand at 61.431 kb on - strand at 61.442 kb on - strand at 61.459 kb on - strand at 61.492 kb on + strand, within rfbG at 61.492 kb on + strand, within rfbG at 61.498 kb on + strand, within rfbG at 61.541 kb on - strand, within rfbG at 61.543 kb on + strand, within rfbG at 61.548 kb on + strand, within rfbG at 61.578 kb on + strand, within rfbG at 61.578 kb on + strand, within rfbG at 61.586 kb on - strand, within rfbG at 61.599 kb on - strand, within rfbG at 61.601 kb on + strand, within rfbG at 61.609 kb on - strand, within rfbG at 61.634 kb on - strand, within rfbG at 61.635 kb on + strand, within rfbG at 61.658 kb on - strand, within rfbG at 61.667 kb on + strand, within rfbG at 61.693 kb on + strand, within rfbG at 61.703 kb on - strand, within rfbG at 61.723 kb on + strand, within rfbG at 61.733 kb on + strand, within rfbG at 61.733 kb on - strand, within rfbG at 61.764 kb on + strand, within rfbG at 61.764 kb on + strand, within rfbG at 61.812 kb on - strand, within rfbG at 61.812 kb on - strand, within rfbG at 61.814 kb on + strand, within rfbG at 61.845 kb on - strand, within rfbG at 61.847 kb on + strand, within rfbG at 61.850 kb on + strand, within rfbG at 61.855 kb on - strand, within rfbG at 61.855 kb on - strand, within rfbG at 61.954 kb on + strand, within rfbG at 61.960 kb on + strand, within rfbG at 61.960 kb on + strand, within rfbG at 61.970 kb on + strand, within rfbG at 61.970 kb on + strand, within rfbG at 61.978 kb on - strand, within rfbG at 61.982 kb on + strand, within rfbG at 61.984 kb on + strand, within rfbG at 61.984 kb on + strand, within rfbG at 61.990 kb on + strand, within rfbG at 61.990 kb on - strand, within rfbG at 61.990 kb on - strand, within rfbG at 61.992 kb on - strand, within rfbG at 62.025 kb on + strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.031 kb on - strand, within rfbG at 62.060 kb on - strand, within rfbG at 62.060 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.086 kb on - strand, within rfbG at 62.096 kb on + strand, within rfbG at 62.115 kb on + strand, within rfbG at 62.130 kb on + strand, within rfbG at 62.147 kb on + strand, within rfbG at 62.170 kb on - strand, within rfbG at 62.176 kb on + strand, within rfbG at 62.184 kb on - strand, within rfbG at 62.184 kb on - strand, within rfbG at 62.195 kb on + strand, within rfbG at 62.203 kb on - strand, within rfbG at 62.205 kb on - strand, within rfbG at 62.205 kb on - strand, within rfbG at 62.231 kb on - strand, within rfbG at 62.236 kb on + strand, within rfbG at 62.249 kb on + strand, within rfbG at 62.284 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand at 62.314 kb on + strand, within rfbG at 62.314 kb on + strand, within rfbG at 62.322 kb on - strand, within rfbG at 62.329 kb on - strand, within rfbG at 62.338 kb on + strand, within rfbG at 62.355 kb on + strand, within rfbG at 62.378 kb on - strand at 62.378 kb on - strand at 62.434 kb on + strand at 62.434 kb on + strand at 62.434 kb on + strand at 62.440 kb on - strand at 62.440 kb on - strand at 62.440 kb on - strand at 62.442 kb on - strand at 62.442 kb on - strand at 62.442 kb on - strand at 62.450 kb on - strand at 62.459 kb on + strand at 62.480 kb on + strand at 62.488 kb on - strand at 62.488 kb on - strand at 62.503 kb on + strand at 62.511 kb on - strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.513 kb on + strand at 62.522 kb on - strand at 62.531 kb on + strand at 62.547 kb on - strand, within rfbF at 62.578 kb on - strand, within rfbF at 62.588 kb on - strand, within rfbF at 62.740 kb on - strand, within rfbF at 62.741 kb on + strand, within rfbF at 62.753 kb on + strand, within rfbF at 62.755 kb on - strand, within rfbF at 62.756 kb on - strand, within rfbF at 62.939 kb on - strand, within rfbF at 62.964 kb on - strand, within rfbF at 63.181 kb on - strand at 63.286 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BG11 with Chloride 15.625 mM remove 60,378 + rfbC Synpcc7942_0058 0.14 -0.2 60,386 - rfbC Synpcc7942_0058 0.16 -0.4 60,396 - rfbC Synpcc7942_0058 0.17 +1.6 60,436 - rfbC Synpcc7942_0058 0.25 +0.6 60,468 + rfbC Synpcc7942_0058 0.31 +0.3 60,476 - rfbC Synpcc7942_0058 0.32 +0.4 60,496 + rfbC Synpcc7942_0058 0.36 +1.1 60,506 + rfbC Synpcc7942_0058 0.38 -1.4 60,512 - rfbC Synpcc7942_0058 0.39 -0.9 60,514 - rfbC Synpcc7942_0058 0.39 -1.1 60,514 - rfbC Synpcc7942_0058 0.39 +0.1 60,514 - rfbC Synpcc7942_0058 0.39 +0.0 60,516 + rfbC Synpcc7942_0058 0.39 -1.1 60,555 - rfbC Synpcc7942_0058 0.47 -0.7 60,573 + rfbC Synpcc7942_0058 0.50 -1.2 60,587 + rfbC Synpcc7942_0058 0.52 -1.4 60,587 + rfbC Synpcc7942_0058 0.52 +1.2 60,589 - rfbC Synpcc7942_0058 0.53 -1.4 60,595 - rfbC Synpcc7942_0058 0.54 -2.0 60,595 - rfbC Synpcc7942_0058 0.54 -0.3 60,621 + rfbC Synpcc7942_0058 0.59 -1.6 60,629 - rfbC Synpcc7942_0058 0.60 -0.4 60,666 + rfbC Synpcc7942_0058 0.67 +0.3 60,682 - rfbC Synpcc7942_0058 0.70 -1.7 60,688 - rfbC Synpcc7942_0058 0.71 -0.8 60,696 - rfbC Synpcc7942_0058 0.72 -1.4 60,704 + rfbC Synpcc7942_0058 0.74 +1.1 60,712 - rfbC Synpcc7942_0058 0.75 -0.8 60,716 + rfbC Synpcc7942_0058 0.76 -2.4 60,724 - rfbC Synpcc7942_0058 0.77 +0.2 60,724 - rfbC Synpcc7942_0058 0.77 -0.8 60,724 - rfbC Synpcc7942_0058 0.77 -0.0 60,813 - -1.8 60,818 - -0.4 60,835 - -1.4 60,953 + Synpcc7942_0059 0.35 +0.5 60,961 - Synpcc7942_0059 0.38 +2.1 60,975 - Synpcc7942_0059 0.44 +0.4 60,996 - Synpcc7942_0059 0.52 +0.1 61,050 - Synpcc7942_0059 0.74 -1.7 61,058 - Synpcc7942_0059 0.78 +1.5 61,083 - Synpcc7942_0059 0.88 +0.1 61,119 + Synpcc7942_0060 0.10 -1.1 61,208 - Synpcc7942_0060 0.42 +2.1 61,225 + Synpcc7942_0060 0.48 -0.7 61,270 - Synpcc7942_0060 0.64 -0.2 61,282 + Synpcc7942_0060 0.68 -0.4 61,290 - Synpcc7942_0060 0.71 -0.6 61,295 - Synpcc7942_0060 0.73 -2.3 61,317 + Synpcc7942_0060 0.81 +1.1 61,361 - +1.0 61,362 - +0.6 61,371 - +3.2 61,387 + -1.3 61,388 - -2.8 61,389 + -2.4 61,395 - +2.3 61,400 + -1.0 61,431 - -2.5 61,442 - -0.2 61,459 - +1.5 61,492 + rfbG Synpcc7942_0061 0.11 +1.0 61,492 + rfbG Synpcc7942_0061 0.11 +0.5 61,498 + rfbG Synpcc7942_0061 0.12 +0.1 61,541 - rfbG Synpcc7942_0061 0.16 -0.2 61,543 + rfbG Synpcc7942_0061 0.16 -0.7 61,548 + rfbG Synpcc7942_0061 0.16 +2.9 61,578 + rfbG Synpcc7942_0061 0.19 -0.6 61,578 + rfbG Synpcc7942_0061 0.19 +0.5 61,586 - rfbG Synpcc7942_0061 0.20 +0.2 61,599 - rfbG Synpcc7942_0061 0.21 -0.7 61,601 + rfbG Synpcc7942_0061 0.21 +0.6 61,609 - rfbG Synpcc7942_0061 0.22 -1.4 61,634 - rfbG Synpcc7942_0061 0.24 +0.4 61,635 + rfbG Synpcc7942_0061 0.24 +0.8 61,658 - rfbG Synpcc7942_0061 0.26 -0.4 61,667 + rfbG Synpcc7942_0061 0.27 +1.1 61,693 + rfbG Synpcc7942_0061 0.30 -1.2 61,703 - rfbG Synpcc7942_0061 0.30 +1.6 61,723 + rfbG Synpcc7942_0061 0.32 -0.3 61,733 + rfbG Synpcc7942_0061 0.33 +1.6 61,733 - rfbG Synpcc7942_0061 0.33 +1.1 61,764 + rfbG Synpcc7942_0061 0.36 +0.8 61,764 + rfbG Synpcc7942_0061 0.36 +0.1 61,812 - rfbG Synpcc7942_0061 0.40 +0.0 61,812 - rfbG Synpcc7942_0061 0.40 -1.7 61,814 + rfbG Synpcc7942_0061 0.40 -0.4 61,845 - rfbG Synpcc7942_0061 0.43 -0.4 61,847 + rfbG Synpcc7942_0061 0.43 -0.7 61,850 + rfbG Synpcc7942_0061 0.44 -1.1 61,855 - rfbG Synpcc7942_0061 0.44 -0.7 61,855 - rfbG Synpcc7942_0061 0.44 +1.0 61,954 + rfbG Synpcc7942_0061 0.53 -2.1 61,960 + rfbG Synpcc7942_0061 0.54 +0.2 61,960 + rfbG Synpcc7942_0061 0.54 -0.6 61,970 + rfbG Synpcc7942_0061 0.55 +0.3 61,970 + rfbG Synpcc7942_0061 0.55 -2.6 61,978 - rfbG Synpcc7942_0061 0.55 +0.9 61,982 + rfbG Synpcc7942_0061 0.56 -0.1 61,984 + rfbG Synpcc7942_0061 0.56 -0.2 61,984 + rfbG Synpcc7942_0061 0.56 +1.0 61,990 + rfbG Synpcc7942_0061 0.56 +1.9 61,990 - rfbG Synpcc7942_0061 0.56 -1.6 61,990 - rfbG Synpcc7942_0061 0.56 +0.6 61,992 - rfbG Synpcc7942_0061 0.57 +0.6 62,025 + rfbG Synpcc7942_0061 0.60 -0.3 62,031 - rfbG Synpcc7942_0061 0.60 +0.2 62,031 - rfbG Synpcc7942_0061 0.60 +0.3 62,031 - rfbG Synpcc7942_0061 0.60 -1.2 62,060 - rfbG Synpcc7942_0061 0.63 -0.3 62,060 - rfbG Synpcc7942_0061 0.63 +0.7 62,086 - rfbG Synpcc7942_0061 0.65 -1.4 62,086 - rfbG Synpcc7942_0061 0.65 -0.4 62,086 - rfbG Synpcc7942_0061 0.65 -0.1 62,086 - rfbG Synpcc7942_0061 0.65 +0.1 62,096 + rfbG Synpcc7942_0061 0.66 +0.1 62,115 + rfbG Synpcc7942_0061 0.68 -2.1 62,130 + rfbG Synpcc7942_0061 0.69 -1.6 62,147 + rfbG Synpcc7942_0061 0.71 -1.2 62,170 - rfbG Synpcc7942_0061 0.73 +0.6 62,176 + rfbG Synpcc7942_0061 0.73 -0.4 62,184 - rfbG Synpcc7942_0061 0.74 -0.7 62,184 - rfbG Synpcc7942_0061 0.74 -0.1 62,195 + rfbG Synpcc7942_0061 0.75 -1.5 62,203 - rfbG Synpcc7942_0061 0.76 -1.1 62,205 - rfbG Synpcc7942_0061 0.76 -1.0 62,205 - rfbG Synpcc7942_0061 0.76 +0.9 62,231 - rfbG Synpcc7942_0061 0.78 -0.7 62,236 + rfbG Synpcc7942_0061 0.79 -0.8 62,249 + rfbG Synpcc7942_0061 0.80 +0.8 62,284 + rfbG Synpcc7942_0061 0.83 -0.9 62,314 + rfbG Synpcc7942_0061 0.86 +0.5 62,314 + rfbG Synpcc7942_0061 0.86 +0.3 62,314 + rfbG Synpcc7942_0061 0.86 -0.2 62,314 + +1.1 62,314 + rfbG Synpcc7942_0061 0.86 -0.6 62,314 + rfbG Synpcc7942_0061 0.86 -0.7 62,322 - rfbG Synpcc7942_0061 0.86 +1.2 62,329 - rfbG Synpcc7942_0061 0.87 -0.4 62,338 + rfbG Synpcc7942_0061 0.88 +0.1 62,355 + rfbG Synpcc7942_0061 0.89 -0.1 62,378 - -1.1 62,378 - +2.2 62,434 + -0.7 62,434 + +0.0 62,434 + -0.5 62,440 - +0.4 62,440 - -1.4 62,440 - -2.2 62,442 - +0.3 62,442 - -1.8 62,442 - +0.4 62,450 - +0.9 62,459 + -0.8 62,480 + -0.8 62,488 - -0.9 62,488 - -1.2 62,503 + -0.3 62,511 - +0.2 62,513 + +0.2 62,513 + +0.1 62,513 + +0.8 62,513 + +0.1 62,513 + -1.9 62,522 - -0.3 62,531 + -1.5 62,547 - rfbF Synpcc7942_0062 0.10 -0.5 62,578 - rfbF Synpcc7942_0062 0.14 -2.1 62,588 - rfbF Synpcc7942_0062 0.15 -0.2 62,740 - rfbF Synpcc7942_0062 0.35 -0.6 62,741 + rfbF Synpcc7942_0062 0.35 -1.6 62,753 + rfbF Synpcc7942_0062 0.37 -1.4 62,755 - rfbF Synpcc7942_0062 0.37 +0.8 62,756 - rfbF Synpcc7942_0062 0.37 -0.4 62,939 - rfbF Synpcc7942_0062 0.61 -0.8 62,964 - rfbF Synpcc7942_0062 0.64 -0.9 63,181 - -0.5 63,286 + -0.7
Or see this region's nucleotide sequence