Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03410 and PGA1_c03420 are separated by 121 nucleotides PGA1_c03420 and PGA1_c03430 overlap by 8 nucleotides PGA1_c03430 and PGA1_c03440 are separated by 119 nucleotides
PGA1_c03410: PGA1_c03410 - EAL domain-containing protein, at 327,954 to 328,796
_c03410
PGA1_c03420: PGA1_c03420 - putative C-5 sterol desaturase, at 328,918 to 329,847
_c03420
PGA1_c03430: PGA1_c03430 - hypothetical protein, at 329,840 to 330,280
_c03430
PGA1_c03440: PGA1_c03440 - disulfide interchange protein tlpA-like protein, at 330,400 to 330,987
_c03440
Position (kb) 328
329
330 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 327.921 kb on - strand at 327.940 kb on + strand at 327.940 kb on - strand at 327.942 kb on + strand at 327.943 kb on - strand at 327.950 kb on - strand at 327.958 kb on - strand at 327.960 kb on - strand at 327.961 kb on + strand at 327.991 kb on - strand at 328.002 kb on + strand at 328.010 kb on - strand at 328.010 kb on - strand at 328.010 kb on - strand at 328.010 kb on - strand at 328.012 kb on + strand at 328.026 kb on + strand at 328.034 kb on - strand at 328.070 kb on + strand, within PGA1_c03410 at 328.316 kb on + strand, within PGA1_c03410 at 328.321 kb on + strand, within PGA1_c03410 at 328.321 kb on + strand, within PGA1_c03410 at 328.324 kb on - strand, within PGA1_c03410 at 328.347 kb on + strand, within PGA1_c03410 at 328.350 kb on - strand, within PGA1_c03410 at 328.350 kb on - strand, within PGA1_c03410 at 328.441 kb on + strand, within PGA1_c03410 at 328.453 kb on - strand, within PGA1_c03410 at 328.463 kb on + strand, within PGA1_c03410 at 328.502 kb on + strand, within PGA1_c03410 at 328.504 kb on - strand, within PGA1_c03410 at 328.507 kb on + strand, within PGA1_c03410 at 328.517 kb on + strand, within PGA1_c03410 at 328.599 kb on + strand, within PGA1_c03410 at 328.614 kb on - strand, within PGA1_c03410 at 328.616 kb on + strand, within PGA1_c03410 at 328.633 kb on + strand, within PGA1_c03410 at 328.730 kb on + strand at 328.738 kb on - strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.798 kb on + strand at 328.806 kb on - strand at 328.806 kb on - strand at 328.806 kb on - strand at 328.806 kb on - strand at 328.806 kb on - strand at 328.807 kb on - strand at 328.816 kb on + strand at 328.816 kb on + strand at 328.816 kb on + strand at 328.816 kb on + strand at 328.816 kb on + strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.824 kb on - strand at 328.829 kb on - strand at 328.829 kb on - strand at 328.829 kb on - strand at 328.834 kb on - strand at 328.839 kb on - strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.841 kb on + strand at 328.849 kb on - strand at 328.849 kb on - strand at 328.915 kb on + strand at 328.916 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.918 kb on - strand at 328.921 kb on + strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.923 kb on - strand at 328.942 kb on - strand at 328.944 kb on + strand at 328.949 kb on + strand at 328.952 kb on - strand at 328.980 kb on + strand at 328.980 kb on + strand at 328.988 kb on - strand at 329.011 kb on + strand, within PGA1_c03420 at 329.099 kb on - strand, within PGA1_c03420 at 329.115 kb on + strand, within PGA1_c03420 at 329.135 kb on - strand, within PGA1_c03420 at 329.139 kb on + strand, within PGA1_c03420 at 329.143 kb on - strand, within PGA1_c03420 at 329.154 kb on - strand, within PGA1_c03420 at 329.169 kb on - strand, within PGA1_c03420 at 329.169 kb on - strand, within PGA1_c03420 at 329.203 kb on - strand, within PGA1_c03420 at 329.217 kb on + strand, within PGA1_c03420 at 329.217 kb on + strand, within PGA1_c03420 at 329.269 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.274 kb on + strand, within PGA1_c03420 at 329.284 kb on + strand, within PGA1_c03420 at 329.295 kb on + strand, within PGA1_c03420 at 329.295 kb on + strand, within PGA1_c03420 at 329.303 kb on - strand, within PGA1_c03420 at 329.305 kb on + strand, within PGA1_c03420 at 329.324 kb on + strand, within PGA1_c03420 at 329.344 kb on - strand, within PGA1_c03420 at 329.358 kb on - strand, within PGA1_c03420 at 329.388 kb on + strand, within PGA1_c03420 at 329.404 kb on + strand, within PGA1_c03420 at 329.412 kb on - strand, within PGA1_c03420 at 329.412 kb on - strand, within PGA1_c03420 at 329.441 kb on - strand, within PGA1_c03420 at 329.447 kb on + strand, within PGA1_c03420 at 329.514 kb on + strand, within PGA1_c03420 at 329.520 kb on - strand, within PGA1_c03420 at 329.522 kb on + strand, within PGA1_c03420 at 329.547 kb on + strand, within PGA1_c03420 at 329.552 kb on + strand, within PGA1_c03420 at 329.558 kb on - strand, within PGA1_c03420 at 329.558 kb on - strand, within PGA1_c03420 at 329.606 kb on + strand, within PGA1_c03420 at 329.629 kb on - strand, within PGA1_c03420 at 329.682 kb on + strand, within PGA1_c03420 at 329.714 kb on - strand, within PGA1_c03420 at 329.721 kb on - strand, within PGA1_c03420 at 329.833 kb on - strand at 329.857 kb on - strand at 329.871 kb on - strand at 329.871 kb on - strand at 329.871 kb on - strand at 329.882 kb on - strand at 329.884 kb on + strand, within PGA1_c03430 at 329.892 kb on - strand, within PGA1_c03430 at 329.921 kb on + strand, within PGA1_c03430 at 329.994 kb on + strand, within PGA1_c03430 at 329.994 kb on + strand, within PGA1_c03430 at 330.007 kb on + strand, within PGA1_c03430 at 330.020 kb on - strand, within PGA1_c03430 at 330.022 kb on + strand, within PGA1_c03430 at 330.030 kb on - strand, within PGA1_c03430 at 330.057 kb on + strand, within PGA1_c03430 at 330.065 kb on - strand, within PGA1_c03430 at 330.065 kb on - strand, within PGA1_c03430 at 330.067 kb on + strand, within PGA1_c03430 at 330.082 kb on - strand, within PGA1_c03430 at 330.094 kb on + strand, within PGA1_c03430 at 330.097 kb on - strand, within PGA1_c03430 at 330.103 kb on + strand, within PGA1_c03430 at 330.155 kb on + strand, within PGA1_c03430 at 330.171 kb on - strand, within PGA1_c03430 at 330.172 kb on - strand, within PGA1_c03430 at 330.196 kb on + strand, within PGA1_c03430 at 330.198 kb on - strand, within PGA1_c03430 at 330.250 kb on + strand at 330.287 kb on + strand at 330.332 kb on - strand at 330.333 kb on - strand at 330.367 kb on + strand at 330.375 kb on - strand at 330.385 kb on + strand at 330.393 kb on - strand at 330.402 kb on + strand at 330.402 kb on + strand at 330.405 kb on - strand at 330.801 kb on + strand, within PGA1_c03440
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 327,921 - +1.5 327,940 + +0.4 327,940 - +0.7 327,942 + -0.6 327,943 - -0.2 327,950 - -1.5 327,958 - +0.3 327,960 - -0.4 327,961 + +0.3 327,991 - -0.2 328,002 + -1.2 328,010 - -1.2 328,010 - +0.2 328,010 - -0.1 328,010 - -1.2 328,012 + -0.1 328,026 + +1.8 328,034 - -2.7 328,070 + PGA1_c03410 0.14 -1.9 328,316 + PGA1_c03410 0.43 -1.0 328,321 + PGA1_c03410 0.44 -0.6 328,321 + PGA1_c03410 0.44 -1.5 328,324 - PGA1_c03410 0.44 +0.2 328,347 + PGA1_c03410 0.47 -0.2 328,350 - PGA1_c03410 0.47 -0.5 328,350 - PGA1_c03410 0.47 -0.9 328,441 + PGA1_c03410 0.58 -0.4 328,453 - PGA1_c03410 0.59 -1.4 328,463 + PGA1_c03410 0.60 -0.9 328,502 + PGA1_c03410 0.65 -2.4 328,504 - PGA1_c03410 0.65 -0.2 328,507 + PGA1_c03410 0.66 -0.5 328,517 + PGA1_c03410 0.67 +0.2 328,599 + PGA1_c03410 0.77 -1.0 328,614 - PGA1_c03410 0.78 -1.4 328,616 + PGA1_c03410 0.79 -0.7 328,633 + PGA1_c03410 0.81 -0.3 328,730 + -0.9 328,738 - -1.3 328,798 + -0.7 328,798 + -0.4 328,798 + -1.8 328,798 + -0.4 328,798 + +0.1 328,798 + -2.4 328,806 - -1.0 328,806 - +0.7 328,806 - -0.2 328,806 - -0.9 328,806 - +0.5 328,807 - -2.8 328,816 + +0.2 328,816 + -2.4 328,816 + +0.2 328,816 + -0.4 328,816 + -1.6 328,824 - -1.1 328,824 - +0.1 328,824 - +0.0 328,824 - -1.1 328,824 - -0.9 328,824 - +1.0 328,824 - +0.3 328,824 - +0.9 328,829 - +0.6 328,829 - -2.3 328,829 - -1.3 328,834 - -0.3 328,839 - +2.1 328,841 + -0.5 328,841 + -0.2 328,841 + -0.2 328,841 + -1.9 328,849 - -2.6 328,849 - -0.2 328,915 + +0.0 328,916 - +0.1 328,918 - +1.1 328,918 - +0.7 328,918 - +1.1 328,918 - -0.2 328,921 + -1.7 328,923 - -0.7 328,923 - -0.3 328,923 - -0.4 328,923 - -0.5 328,923 - +0.4 328,942 - +1.1 328,944 + -1.5 328,949 + -2.5 328,952 - +0.9 328,980 + -1.1 328,980 + +0.1 328,988 - +0.6 329,011 + PGA1_c03420 0.10 +1.3 329,099 - PGA1_c03420 0.19 +0.8 329,115 + PGA1_c03420 0.21 -1.0 329,135 - PGA1_c03420 0.23 -0.1 329,139 + PGA1_c03420 0.24 -0.0 329,143 - PGA1_c03420 0.24 -0.9 329,154 - PGA1_c03420 0.25 -2.6 329,169 - PGA1_c03420 0.27 +0.5 329,169 - PGA1_c03420 0.27 -0.0 329,203 - PGA1_c03420 0.31 -1.7 329,217 + PGA1_c03420 0.32 -1.7 329,217 + PGA1_c03420 0.32 +0.9 329,269 + PGA1_c03420 0.38 -0.3 329,274 + PGA1_c03420 0.38 +0.2 329,274 + PGA1_c03420 0.38 +1.2 329,274 + PGA1_c03420 0.38 +1.6 329,284 + PGA1_c03420 0.39 +0.9 329,295 + PGA1_c03420 0.41 +1.0 329,295 + PGA1_c03420 0.41 -0.7 329,303 - PGA1_c03420 0.41 +1.1 329,305 + PGA1_c03420 0.42 +0.2 329,324 + PGA1_c03420 0.44 -0.3 329,344 - PGA1_c03420 0.46 -2.1 329,358 - PGA1_c03420 0.47 -1.7 329,388 + PGA1_c03420 0.51 -1.2 329,404 + PGA1_c03420 0.52 +0.6 329,412 - PGA1_c03420 0.53 -0.6 329,412 - PGA1_c03420 0.53 +0.6 329,441 - PGA1_c03420 0.56 +1.3 329,447 + PGA1_c03420 0.57 +0.7 329,514 + PGA1_c03420 0.64 +0.7 329,520 - PGA1_c03420 0.65 +0.5 329,522 + PGA1_c03420 0.65 -1.4 329,547 + PGA1_c03420 0.68 +1.0 329,552 + PGA1_c03420 0.68 -0.7 329,558 - PGA1_c03420 0.69 -0.2 329,558 - PGA1_c03420 0.69 -0.8 329,606 + PGA1_c03420 0.74 -1.7 329,629 - PGA1_c03420 0.76 +0.4 329,682 + PGA1_c03420 0.82 +0.5 329,714 - PGA1_c03420 0.86 -1.2 329,721 - PGA1_c03420 0.86 +0.5 329,833 - +0.8 329,857 - +0.9 329,871 - +0.8 329,871 - +1.5 329,871 - -1.7 329,882 - -0.5 329,884 + PGA1_c03430 0.10 +0.3 329,892 - PGA1_c03430 0.12 -0.4 329,921 + PGA1_c03430 0.18 +1.1 329,994 + PGA1_c03430 0.35 -0.8 329,994 + PGA1_c03430 0.35 -0.6 330,007 + PGA1_c03430 0.38 -0.8 330,020 - PGA1_c03430 0.41 +0.4 330,022 + PGA1_c03430 0.41 +0.3 330,030 - PGA1_c03430 0.43 +0.2 330,057 + PGA1_c03430 0.49 +0.4 330,065 - PGA1_c03430 0.51 -0.5 330,065 - PGA1_c03430 0.51 -0.2 330,067 + PGA1_c03430 0.51 -0.5 330,082 - PGA1_c03430 0.55 +0.3 330,094 + PGA1_c03430 0.58 -1.4 330,097 - PGA1_c03430 0.58 +0.5 330,103 + PGA1_c03430 0.60 -0.7 330,155 + PGA1_c03430 0.71 +0.5 330,171 - PGA1_c03430 0.75 +0.2 330,172 - PGA1_c03430 0.75 -0.5 330,196 + PGA1_c03430 0.81 -1.9 330,198 - PGA1_c03430 0.81 +1.1 330,250 + +0.4 330,287 + +0.4 330,332 - -1.9 330,333 - +0.4 330,367 + +0.5 330,375 - -1.8 330,385 + +0.4 330,393 - -0.1 330,402 + -0.7 330,402 + -0.5 330,405 - -1.9 330,801 + PGA1_c03440 0.68 -0.9
Or see this region's nucleotide sequence