Experiment: Putrescine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c19150 and PGA1_c19160 are separated by 58 nucleotides PGA1_c19160 and PGA1_c19170 are separated by 85 nucleotides PGA1_c19170 and PGA1_c19180 are separated by 233 nucleotides
PGA1_c19150: PGA1_c19150 - sarcosine oxidase subunit alpha, at 1,987,077 to 1,990,109
_c19150
PGA1_c19160: PGA1_c19160 - sarcosine oxidase 2 subunit delta, at 1,990,168 to 1,990,497
_c19160
PGA1_c19170: PGA1_c19170 - sarcosine oxidase subunit beta, at 1,990,583 to 1,991,827
_c19170
PGA1_c19180: PGA1_c19180 - cytochrome c-type biogenesis protein CycH, at 1,992,061 to 1,993,317
_c19180
Position (kb)
1990
1991
1992 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1989.631 kb on + strand, within PGA1_c19150 at 1989.633 kb on + strand, within PGA1_c19150 at 1989.639 kb on - strand, within PGA1_c19150 at 1989.662 kb on + strand, within PGA1_c19150 at 1989.705 kb on - strand, within PGA1_c19150 at 1989.756 kb on - strand, within PGA1_c19150 at 1989.791 kb on + strand, within PGA1_c19150 at 1989.800 kb on - strand, within PGA1_c19150 at 1989.912 kb on - strand at 1989.970 kb on + strand at 1989.970 kb on + strand at 1990.013 kb on + strand at 1990.026 kb on - strand at 1990.031 kb on - strand at 1990.069 kb on - strand at 1990.069 kb on - strand at 1990.122 kb on + strand at 1990.238 kb on + strand, within PGA1_c19160 at 1990.244 kb on - strand, within PGA1_c19160 at 1990.244 kb on - strand, within PGA1_c19160 at 1990.244 kb on - strand, within PGA1_c19160 at 1990.244 kb on - strand, within PGA1_c19160 at 1990.244 kb on - strand, within PGA1_c19160 at 1990.244 kb on - strand, within PGA1_c19160 at 1990.244 kb on - strand, within PGA1_c19160 at 1990.256 kb on - strand, within PGA1_c19160 at 1990.277 kb on - strand, within PGA1_c19160 at 1990.277 kb on - strand, within PGA1_c19160 at 1990.277 kb on - strand, within PGA1_c19160 at 1990.277 kb on - strand at 1990.303 kb on - strand, within PGA1_c19160 at 1990.303 kb on - strand, within PGA1_c19160 at 1990.323 kb on - strand, within PGA1_c19160 at 1990.335 kb on - strand, within PGA1_c19160 at 1990.349 kb on + strand, within PGA1_c19160 at 1990.349 kb on + strand, within PGA1_c19160 at 1990.349 kb on + strand, within PGA1_c19160 at 1990.349 kb on + strand, within PGA1_c19160 at 1990.357 kb on - strand, within PGA1_c19160 at 1990.357 kb on - strand, within PGA1_c19160 at 1990.357 kb on - strand, within PGA1_c19160 at 1990.402 kb on + strand, within PGA1_c19160 at 1990.497 kb on - strand at 1990.497 kb on - strand at 1990.497 kb on - strand at 1990.497 kb on - strand at 1990.507 kb on - strand at 1990.517 kb on - strand at 1990.541 kb on - strand at 1990.652 kb on - strand at 1990.682 kb on - strand at 1990.720 kb on + strand, within PGA1_c19170 at 1990.736 kb on + strand, within PGA1_c19170 at 1990.744 kb on - strand, within PGA1_c19170 at 1990.853 kb on - strand, within PGA1_c19170 at 1990.871 kb on - strand, within PGA1_c19170 at 1990.923 kb on - strand, within PGA1_c19170 at 1990.923 kb on - strand, within PGA1_c19170 at 1990.941 kb on - strand, within PGA1_c19170 at 1990.995 kb on + strand, within PGA1_c19170 at 1991.003 kb on - strand, within PGA1_c19170 at 1991.003 kb on - strand, within PGA1_c19170 at 1991.025 kb on + strand, within PGA1_c19170 at 1991.043 kb on - strand, within PGA1_c19170 at 1991.056 kb on + strand, within PGA1_c19170 at 1991.216 kb on + strand, within PGA1_c19170 at 1991.226 kb on - strand, within PGA1_c19170 at 1991.270 kb on - strand, within PGA1_c19170 at 1991.431 kb on + strand, within PGA1_c19170 at 1991.454 kb on - strand, within PGA1_c19170 at 1991.457 kb on + strand, within PGA1_c19170 at 1991.546 kb on + strand, within PGA1_c19170 at 1991.595 kb on - strand, within PGA1_c19170 at 1991.612 kb on - strand, within PGA1_c19170 at 1991.778 kb on + strand at 1991.860 kb on - strand at 1991.868 kb on - strand at 1991.876 kb on + strand at 1991.884 kb on - strand at 1991.922 kb on - strand at 1991.978 kb on + strand at 1992.031 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Putrescine (N) remove 1,989,631 + PGA1_c19150 0.84 -1.2 1,989,633 + PGA1_c19150 0.84 -2.6 1,989,639 - PGA1_c19150 0.84 -3.4 1,989,662 + PGA1_c19150 0.85 -2.1 1,989,705 - PGA1_c19150 0.87 -2.4 1,989,756 - PGA1_c19150 0.88 -1.0 1,989,791 + PGA1_c19150 0.89 -1.8 1,989,800 - PGA1_c19150 0.90 -1.1 1,989,912 - -2.0 1,989,970 + -2.6 1,989,970 + -1.6 1,990,013 + -2.4 1,990,026 - -0.5 1,990,031 - -1.8 1,990,069 - -0.6 1,990,069 - -1.3 1,990,122 + +0.1 1,990,238 + PGA1_c19160 0.21 -2.0 1,990,244 - PGA1_c19160 0.23 -1.8 1,990,244 - PGA1_c19160 0.23 -1.3 1,990,244 - PGA1_c19160 0.23 -0.1 1,990,244 - PGA1_c19160 0.23 -1.3 1,990,244 - PGA1_c19160 0.23 -2.2 1,990,244 - PGA1_c19160 0.23 +0.9 1,990,244 - PGA1_c19160 0.23 +0.1 1,990,256 - PGA1_c19160 0.27 -1.3 1,990,277 - PGA1_c19160 0.33 -1.9 1,990,277 - PGA1_c19160 0.33 -0.6 1,990,277 - PGA1_c19160 0.33 -2.0 1,990,277 - -1.3 1,990,303 - PGA1_c19160 0.41 -2.2 1,990,303 - PGA1_c19160 0.41 -2.6 1,990,323 - PGA1_c19160 0.47 -1.8 1,990,335 - PGA1_c19160 0.51 -3.6 1,990,349 + PGA1_c19160 0.55 -1.0 1,990,349 + PGA1_c19160 0.55 -3.7 1,990,349 + PGA1_c19160 0.55 -1.3 1,990,349 + PGA1_c19160 0.55 -1.6 1,990,357 - PGA1_c19160 0.57 -2.4 1,990,357 - PGA1_c19160 0.57 -0.6 1,990,357 - PGA1_c19160 0.57 -1.2 1,990,402 + PGA1_c19160 0.71 -2.2 1,990,497 - -1.4 1,990,497 - -1.0 1,990,497 - -0.1 1,990,497 - -1.6 1,990,507 - +0.3 1,990,517 - +0.2 1,990,541 - -0.9 1,990,652 - -1.7 1,990,682 - -2.2 1,990,720 + PGA1_c19170 0.11 -2.6 1,990,736 + PGA1_c19170 0.12 -2.5 1,990,744 - PGA1_c19170 0.13 -1.6 1,990,853 - PGA1_c19170 0.22 -2.7 1,990,871 - PGA1_c19170 0.23 -2.2 1,990,923 - PGA1_c19170 0.27 -2.3 1,990,923 - PGA1_c19170 0.27 -2.1 1,990,941 - PGA1_c19170 0.29 -1.3 1,990,995 + PGA1_c19170 0.33 +0.7 1,991,003 - PGA1_c19170 0.34 -2.4 1,991,003 - PGA1_c19170 0.34 -0.1 1,991,025 + PGA1_c19170 0.36 -0.6 1,991,043 - PGA1_c19170 0.37 -1.9 1,991,056 + PGA1_c19170 0.38 -2.5 1,991,216 + PGA1_c19170 0.51 -2.9 1,991,226 - PGA1_c19170 0.52 -3.5 1,991,270 - PGA1_c19170 0.55 -1.3 1,991,431 + PGA1_c19170 0.68 -2.1 1,991,454 - PGA1_c19170 0.70 -2.6 1,991,457 + PGA1_c19170 0.70 -3.0 1,991,546 + PGA1_c19170 0.77 -1.5 1,991,595 - PGA1_c19170 0.81 -2.0 1,991,612 - PGA1_c19170 0.83 -1.8 1,991,778 + -2.4 1,991,860 - -1.8 1,991,868 - -3.0 1,991,876 + -2.0 1,991,884 - -0.1 1,991,922 - +0.5 1,991,978 + +0.8 1,992,031 + -0.0
Or see this region's nucleotide sequence