Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tRNA-Ser and BT4681 are separated by 828 nucleotides BT4681 and BT4682 are separated by 31 nucleotides
BT_t70: tRNA-Ser - tRNA-Ser (RefSeq), at 6,138,247 to 6,138,331
tRNA-Ser
BT4681: BT4681 - beta-hexosaminidase precursor (NCBI ptt file), at 6,139,160 to 6,140,770
BT4681
BT4682: BT4682 - conserved hypothetical protein (NCBI ptt file), at 6,140,802 to 6,143,240
BT4682
Position (kb)
6139
6140
6141 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 6138.490 kb on - strand at 6138.588 kb on - strand at 6138.600 kb on + strand at 6138.657 kb on - strand at 6138.663 kb on - strand at 6138.671 kb on + strand at 6138.694 kb on - strand at 6138.700 kb on + strand at 6138.701 kb on - strand at 6138.711 kb on + strand at 6138.788 kb on - strand at 6138.820 kb on + strand at 6138.865 kb on + strand at 6138.932 kb on - strand at 6138.936 kb on - strand at 6139.037 kb on + strand at 6139.061 kb on + strand at 6139.110 kb on + strand at 6139.136 kb on - strand at 6139.148 kb on - strand at 6139.149 kb on + strand at 6139.217 kb on - strand at 6139.321 kb on + strand, within BT4681 at 6139.337 kb on + strand, within BT4681 at 6139.349 kb on - strand, within BT4681 at 6139.576 kb on + strand, within BT4681 at 6139.577 kb on - strand, within BT4681 at 6139.635 kb on + strand, within BT4681 at 6139.763 kb on + strand, within BT4681 at 6139.764 kb on - strand, within BT4681 at 6139.764 kb on - strand, within BT4681 at 6139.815 kb on - strand, within BT4681 at 6139.841 kb on + strand, within BT4681 at 6139.900 kb on - strand, within BT4681 at 6139.949 kb on + strand, within BT4681 at 6139.949 kb on + strand, within BT4681 at 6139.976 kb on - strand, within BT4681 at 6139.976 kb on - strand, within BT4681 at 6139.976 kb on - strand, within BT4681 at 6139.993 kb on - strand, within BT4681 at 6140.005 kb on + strand, within BT4681 at 6140.006 kb on - strand, within BT4681 at 6140.067 kb on + strand, within BT4681 at 6140.100 kb on + strand, within BT4681 at 6140.100 kb on + strand, within BT4681 at 6140.104 kb on - strand, within BT4681 at 6140.194 kb on - strand, within BT4681 at 6140.337 kb on - strand, within BT4681 at 6140.337 kb on - strand, within BT4681 at 6140.368 kb on + strand, within BT4681 at 6140.368 kb on + strand, within BT4681 at 6140.369 kb on - strand, within BT4681 at 6140.390 kb on + strand, within BT4681 at 6140.412 kb on - strand, within BT4681 at 6140.426 kb on + strand, within BT4681 at 6140.471 kb on + strand, within BT4681 at 6140.472 kb on - strand, within BT4681 at 6140.472 kb on - strand, within BT4681 at 6140.537 kb on + strand, within BT4681 at 6140.554 kb on + strand, within BT4681 at 6140.554 kb on + strand, within BT4681 at 6140.634 kb on - strand at 6140.675 kb on - strand at 6140.675 kb on - strand at 6140.728 kb on - strand at 6140.753 kb on - strand at 6140.782 kb on + strand at 6140.782 kb on + strand at 6140.782 kb on + strand at 6140.783 kb on - strand at 6140.788 kb on - strand at 6140.795 kb on + strand at 6140.795 kb on + strand at 6140.841 kb on + strand at 6140.849 kb on - strand at 6141.070 kb on - strand, within BT4682 at 6141.072 kb on + strand, within BT4682 at 6141.207 kb on + strand, within BT4682 at 6141.357 kb on + strand, within BT4682 at 6141.427 kb on - strand, within BT4682 at 6141.607 kb on + strand, within BT4682 at 6141.663 kb on - strand, within BT4682
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 6,138,490 - -2.0 6,138,588 - -1.9 6,138,600 + -1.4 6,138,657 - -0.0 6,138,663 - +1.6 6,138,671 + -1.0 6,138,694 - -3.1 6,138,700 + -1.0 6,138,701 - -0.0 6,138,711 + -1.0 6,138,788 - -2.3 6,138,820 + -2.6 6,138,865 + -2.6 6,138,932 - -1.9 6,138,936 - -0.0 6,139,037 + +0.6 6,139,061 + +3.2 6,139,110 + -2.5 6,139,136 - -2.3 6,139,148 - -3.6 6,139,149 + -1.6 6,139,217 - -0.8 6,139,321 + BT4681 0.10 -0.0 6,139,337 + BT4681 0.11 -1.0 6,139,349 - BT4681 0.12 +0.6 6,139,576 + BT4681 0.26 +1.6 6,139,577 - BT4681 0.26 -0.0 6,139,635 + BT4681 0.29 -1.0 6,139,763 + BT4681 0.37 -0.0 6,139,764 - BT4681 0.37 -2.1 6,139,764 - BT4681 0.37 -0.2 6,139,815 - BT4681 0.41 -1.6 6,139,841 + BT4681 0.42 -1.0 6,139,900 - BT4681 0.46 -0.9 6,139,949 + BT4681 0.49 +0.4 6,139,949 + BT4681 0.49 +1.0 6,139,976 - BT4681 0.51 -0.0 6,139,976 - BT4681 0.51 -1.6 6,139,976 - BT4681 0.51 +1.0 6,139,993 - BT4681 0.52 -1.5 6,140,005 + BT4681 0.52 -3.1 6,140,006 - BT4681 0.53 +1.0 6,140,067 + BT4681 0.56 -0.0 6,140,100 + BT4681 0.58 -2.8 6,140,100 + BT4681 0.58 +0.3 6,140,104 - BT4681 0.59 -1.0 6,140,194 - BT4681 0.64 -1.2 6,140,337 - BT4681 0.73 -1.2 6,140,337 - BT4681 0.73 -3.6 6,140,368 + BT4681 0.75 +2.8 6,140,368 + BT4681 0.75 -1.6 6,140,369 - BT4681 0.75 -1.6 6,140,390 + BT4681 0.76 -2.0 6,140,412 - BT4681 0.78 -1.0 6,140,426 + BT4681 0.79 +1.0 6,140,471 + BT4681 0.81 -1.0 6,140,472 - BT4681 0.81 -0.9 6,140,472 - BT4681 0.81 -2.6 6,140,537 + BT4681 0.85 -2.3 6,140,554 + BT4681 0.87 -2.0 6,140,554 + BT4681 0.87 -1.6 6,140,634 - -0.0 6,140,675 - -0.0 6,140,675 - +1.6 6,140,728 - -2.0 6,140,753 - -0.0 6,140,782 + -0.9 6,140,782 + -2.2 6,140,782 + -2.4 6,140,783 - -1.0 6,140,788 - -1.0 6,140,795 + -2.3 6,140,795 + -2.2 6,140,841 + -0.6 6,140,849 - -0.0 6,141,070 - BT4682 0.11 -1.6 6,141,072 + BT4682 0.11 -1.6 6,141,207 + BT4682 0.17 +1.0 6,141,357 + BT4682 0.23 -2.6 6,141,427 - BT4682 0.26 +1.7 6,141,607 + BT4682 0.33 -2.0 6,141,663 - BT4682 0.35 +1.6
Or see this region's nucleotide sequence