Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4200 and BT4201 are separated by 101 nucleotides BT4201 and BT4202 are separated by 114 nucleotides BT4202 and BT4203 overlap by 28 nucleotides
BT4200: BT4200 - conserved hypothetical protein (NCBI ptt file), at 5,534,649 to 5,536,781
BT4200
BT4201: BT4201 - conserved hypothetical protein (NCBI ptt file), at 5,536,883 to 5,539,324
BT4201
BT4202: BT4202 - hypothetical protein (NCBI ptt file), at 5,539,439 to 5,539,882
BT4202
BT4203: BT4203 - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file), at 5,539,855 to 5,540,781
BT4203
Position (kb) 5536
5537
5538
5539
5540 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 5535.889 kb on - strand, within BT4200 at 5535.939 kb on - strand, within BT4200 at 5535.940 kb on + strand, within BT4200 at 5535.940 kb on + strand, within BT4200 at 5535.966 kb on - strand, within BT4200 at 5535.996 kb on + strand, within BT4200 at 5535.996 kb on + strand, within BT4200 at 5536.041 kb on - strand, within BT4200 at 5536.061 kb on + strand, within BT4200 at 5536.135 kb on - strand, within BT4200 at 5536.217 kb on + strand, within BT4200 at 5536.264 kb on - strand, within BT4200 at 5536.321 kb on - strand, within BT4200 at 5536.356 kb on - strand, within BT4200 at 5536.394 kb on + strand, within BT4200 at 5536.418 kb on - strand, within BT4200 at 5536.418 kb on - strand, within BT4200 at 5536.420 kb on + strand, within BT4200 at 5536.437 kb on - strand, within BT4200 at 5536.500 kb on - strand, within BT4200 at 5536.568 kb on - strand at 5536.585 kb on + strand at 5536.586 kb on - strand at 5536.593 kb on - strand at 5536.608 kb on - strand at 5536.625 kb on + strand at 5536.626 kb on - strand at 5536.628 kb on + strand at 5536.632 kb on + strand at 5536.633 kb on - strand at 5536.784 kb on + strand at 5536.794 kb on + strand at 5536.797 kb on - strand at 5536.797 kb on - strand at 5536.797 kb on - strand at 5536.801 kb on - strand at 5536.828 kb on + strand at 5536.855 kb on + strand at 5536.869 kb on - strand at 5536.904 kb on - strand at 5536.938 kb on - strand at 5536.960 kb on - strand at 5537.038 kb on - strand at 5537.098 kb on - strand at 5537.207 kb on - strand, within BT4201 at 5537.209 kb on - strand, within BT4201 at 5537.232 kb on - strand, within BT4201 at 5537.248 kb on - strand, within BT4201 at 5537.250 kb on + strand, within BT4201 at 5537.251 kb on - strand, within BT4201 at 5537.414 kb on - strand, within BT4201 at 5537.453 kb on - strand, within BT4201 at 5537.453 kb on - strand, within BT4201 at 5537.459 kb on - strand, within BT4201 at 5537.486 kb on - strand, within BT4201 at 5537.487 kb on + strand, within BT4201 at 5537.488 kb on - strand, within BT4201 at 5537.488 kb on - strand, within BT4201 at 5537.546 kb on - strand, within BT4201 at 5537.665 kb on + strand, within BT4201 at 5537.709 kb on - strand, within BT4201 at 5537.715 kb on - strand, within BT4201 at 5537.715 kb on - strand, within BT4201 at 5537.715 kb on - strand, within BT4201 at 5537.715 kb on - strand, within BT4201 at 5537.715 kb on - strand, within BT4201 at 5537.863 kb on + strand, within BT4201 at 5537.895 kb on + strand, within BT4201 at 5537.935 kb on - strand, within BT4201 at 5537.943 kb on - strand, within BT4201 at 5537.978 kb on - strand, within BT4201 at 5537.978 kb on - strand, within BT4201 at 5537.998 kb on + strand, within BT4201 at 5538.050 kb on - strand, within BT4201 at 5538.058 kb on - strand, within BT4201 at 5538.073 kb on + strand, within BT4201 at 5538.086 kb on - strand, within BT4201 at 5538.119 kb on - strand, within BT4201 at 5538.119 kb on - strand, within BT4201 at 5538.127 kb on - strand, within BT4201 at 5538.127 kb on - strand, within BT4201 at 5538.199 kb on + strand, within BT4201 at 5538.200 kb on - strand, within BT4201 at 5538.206 kb on - strand, within BT4201 at 5538.208 kb on - strand, within BT4201 at 5538.211 kb on - strand, within BT4201 at 5538.211 kb on - strand, within BT4201 at 5538.263 kb on - strand, within BT4201 at 5538.268 kb on + strand, within BT4201 at 5538.312 kb on - strand, within BT4201 at 5538.326 kb on - strand, within BT4201 at 5538.421 kb on - strand, within BT4201 at 5538.479 kb on - strand, within BT4201 at 5538.547 kb on - strand, within BT4201 at 5538.578 kb on + strand, within BT4201 at 5538.637 kb on + strand, within BT4201 at 5538.654 kb on - strand, within BT4201 at 5538.788 kb on - strand, within BT4201 at 5538.866 kb on - strand, within BT4201 at 5538.878 kb on + strand, within BT4201 at 5538.968 kb on - strand, within BT4201 at 5538.971 kb on + strand, within BT4201 at 5538.977 kb on - strand, within BT4201 at 5538.994 kb on + strand, within BT4201 at 5539.015 kb on + strand, within BT4201 at 5539.015 kb on + strand, within BT4201 at 5539.016 kb on - strand, within BT4201 at 5539.016 kb on - strand, within BT4201 at 5539.074 kb on + strand, within BT4201 at 5539.171 kb on - strand at 5539.189 kb on + strand at 5539.189 kb on + strand at 5539.190 kb on - strand at 5539.190 kb on - strand at 5539.190 kb on - strand at 5539.195 kb on - strand at 5539.287 kb on + strand at 5539.301 kb on - strand at 5539.301 kb on - strand at 5539.330 kb on + strand at 5539.331 kb on - strand at 5539.572 kb on - strand, within BT4202 at 5539.573 kb on + strand, within BT4202 at 5539.573 kb on + strand, within BT4202 at 5539.574 kb on - strand, within BT4202 at 5539.578 kb on + strand, within BT4202 at 5539.579 kb on - strand, within BT4202 at 5539.599 kb on - strand, within BT4202 at 5539.616 kb on + strand, within BT4202 at 5539.617 kb on - strand, within BT4202 at 5539.704 kb on - strand, within BT4202 at 5539.780 kb on + strand, within BT4202 at 5539.835 kb on - strand, within BT4202 at 5539.835 kb on - strand, within BT4202 at 5539.835 kb on - strand at 5539.905 kb on - strand at 5539.912 kb on + strand at 5540.260 kb on - strand, within BT4203
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 5,535,889 - BT4200 0.58 -3.2 5,535,939 - BT4200 0.60 -2.0 5,535,940 + BT4200 0.61 +0.0 5,535,940 + BT4200 0.61 +0.0 5,535,966 - BT4200 0.62 -1.7 5,535,996 + BT4200 0.63 +0.0 5,535,996 + BT4200 0.63 -2.0 5,536,041 - BT4200 0.65 +0.0 5,536,061 + BT4200 0.66 +1.0 5,536,135 - BT4200 0.70 -0.6 5,536,217 + BT4200 0.74 +2.0 5,536,264 - BT4200 0.76 -2.3 5,536,321 - BT4200 0.78 +0.0 5,536,356 - BT4200 0.80 -1.0 5,536,394 + BT4200 0.82 -0.7 5,536,418 - BT4200 0.83 -1.0 5,536,418 - BT4200 0.83 -1.6 5,536,420 + BT4200 0.83 -2.6 5,536,437 - BT4200 0.84 -0.6 5,536,500 - BT4200 0.87 -3.2 5,536,568 - -0.2 5,536,585 + +2.0 5,536,586 - -2.0 5,536,593 - -1.7 5,536,608 - +2.8 5,536,625 + -1.3 5,536,626 - -4.3 5,536,628 + -2.2 5,536,632 + -2.8 5,536,633 - -1.6 5,536,784 + -0.8 5,536,794 + -2.0 5,536,797 - +0.0 5,536,797 - -1.6 5,536,797 - -3.0 5,536,801 - +1.1 5,536,828 + +0.0 5,536,855 + -3.1 5,536,869 - +0.0 5,536,904 - +1.2 5,536,938 - -0.9 5,536,960 - -3.3 5,537,038 - -3.0 5,537,098 - -1.6 5,537,207 - BT4201 0.13 -1.6 5,537,209 - BT4201 0.13 +0.0 5,537,232 - BT4201 0.14 +1.6 5,537,248 - BT4201 0.15 -2.0 5,537,250 + BT4201 0.15 +0.0 5,537,251 - BT4201 0.15 +0.0 5,537,414 - BT4201 0.22 -3.3 5,537,453 - BT4201 0.23 -0.6 5,537,453 - BT4201 0.23 -0.3 5,537,459 - BT4201 0.24 -2.9 5,537,486 - BT4201 0.25 -1.8 5,537,487 + BT4201 0.25 -2.1 5,537,488 - BT4201 0.25 -0.6 5,537,488 - BT4201 0.25 -1.9 5,537,546 - BT4201 0.27 -2.0 5,537,665 + BT4201 0.32 -2.3 5,537,709 - BT4201 0.34 -0.5 5,537,715 - BT4201 0.34 -1.0 5,537,715 - BT4201 0.34 +0.0 5,537,715 - BT4201 0.34 -3.0 5,537,715 - BT4201 0.34 -2.3 5,537,715 - BT4201 0.34 -1.2 5,537,863 + BT4201 0.40 +1.0 5,537,895 + BT4201 0.41 -0.6 5,537,935 - BT4201 0.43 -2.8 5,537,943 - BT4201 0.43 -2.3 5,537,978 - BT4201 0.45 +0.0 5,537,978 - BT4201 0.45 -2.3 5,537,998 + BT4201 0.46 -2.6 5,538,050 - BT4201 0.48 -2.0 5,538,058 - BT4201 0.48 +2.8 5,538,073 + BT4201 0.49 -1.6 5,538,086 - BT4201 0.49 -1.0 5,538,119 - BT4201 0.51 -1.2 5,538,119 - BT4201 0.51 +0.0 5,538,127 - BT4201 0.51 -2.4 5,538,127 - BT4201 0.51 -3.0 5,538,199 + BT4201 0.54 +1.6 5,538,200 - BT4201 0.54 -1.3 5,538,206 - BT4201 0.54 -1.1 5,538,208 - BT4201 0.54 -1.0 5,538,211 - BT4201 0.54 +3.7 5,538,211 - BT4201 0.54 +1.0 5,538,263 - BT4201 0.57 -3.2 5,538,268 + BT4201 0.57 -1.0 5,538,312 - BT4201 0.59 -0.6 5,538,326 - BT4201 0.59 -0.6 5,538,421 - BT4201 0.63 -2.6 5,538,479 - BT4201 0.65 +0.0 5,538,547 - BT4201 0.68 +0.0 5,538,578 + BT4201 0.69 -2.0 5,538,637 + BT4201 0.72 +0.0 5,538,654 - BT4201 0.73 -2.5 5,538,788 - BT4201 0.78 -1.0 5,538,866 - BT4201 0.81 -1.0 5,538,878 + BT4201 0.82 -1.0 5,538,968 - BT4201 0.85 -2.1 5,538,971 + BT4201 0.86 +3.0 5,538,977 - BT4201 0.86 -1.6 5,538,994 + BT4201 0.86 -3.5 5,539,015 + BT4201 0.87 +1.5 5,539,015 + BT4201 0.87 -2.5 5,539,016 - BT4201 0.87 -2.3 5,539,016 - BT4201 0.87 -2.3 5,539,074 + BT4201 0.90 +1.6 5,539,171 - -2.6 5,539,189 + -2.0 5,539,189 + -2.0 5,539,190 - -0.4 5,539,190 - +0.0 5,539,190 - -2.8 5,539,195 - -2.4 5,539,287 + -2.0 5,539,301 - +0.0 5,539,301 - -1.3 5,539,330 + -1.0 5,539,331 - +0.8 5,539,572 - BT4202 0.30 -3.2 5,539,573 + BT4202 0.30 -1.8 5,539,573 + BT4202 0.30 -1.4 5,539,574 - BT4202 0.30 -1.2 5,539,578 + BT4202 0.31 -1.4 5,539,579 - BT4202 0.32 +0.5 5,539,599 - BT4202 0.36 +1.0 5,539,616 + BT4202 0.40 +0.0 5,539,617 - BT4202 0.40 +2.0 5,539,704 - BT4202 0.60 -2.2 5,539,780 + BT4202 0.77 -0.5 5,539,835 - BT4202 0.89 -1.6 5,539,835 - BT4202 0.89 -0.7 5,539,835 - -1.0 5,539,905 - +1.0 5,539,912 + +0.0 5,540,260 - BT4203 0.44 -1.0
Or see this region's nucleotide sequence