Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4145 and BT4146 overlap by 1 nucleotides BT4146 and BT4147 are separated by 89 nucleotides BT4147 and BT4148 overlap by 50 nucleotides
BT4145: BT4145 - conserved hypothetical protein (NCBI ptt file), at 5,441,391 to 5,444,165
BT4145
BT4146: BT4146 - exo-poly-alpha-D-galacturonosidase precursor (NCBI ptt file), at 5,444,165 to 5,445,568
BT4146
BT4147: BT4147 - hypothetical protein (NCBI ptt file), at 5,445,658 to 5,446,248
BT4147
BT4148: BT4148 - hypothetical protein (NCBI ptt file), at 5,446,199 to 5,446,414
BT4148
Position (kb)
5444
5445
5446 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5443.346 kb on + strand, within BT4145 at 5443.411 kb on + strand, within BT4145 at 5443.448 kb on + strand, within BT4145 at 5443.469 kb on + strand, within BT4145 at 5443.518 kb on + strand, within BT4145 at 5443.523 kb on - strand, within BT4145 at 5443.576 kb on + strand, within BT4145 at 5443.576 kb on + strand, within BT4145 at 5443.576 kb on + strand, within BT4145 at 5443.646 kb on + strand, within BT4145 at 5443.646 kb on + strand, within BT4145 at 5443.646 kb on + strand, within BT4145 at 5443.769 kb on - strand, within BT4145 at 5443.954 kb on - strand at 5443.954 kb on - strand at 5443.960 kb on + strand at 5444.002 kb on + strand at 5444.003 kb on - strand at 5444.018 kb on - strand at 5444.145 kb on + strand at 5444.146 kb on - strand at 5444.180 kb on - strand at 5444.187 kb on + strand at 5444.187 kb on + strand at 5444.270 kb on + strand at 5444.292 kb on + strand at 5444.292 kb on + strand at 5444.398 kb on - strand, within BT4146 at 5444.400 kb on + strand, within BT4146 at 5444.400 kb on + strand, within BT4146 at 5444.400 kb on + strand, within BT4146 at 5444.401 kb on - strand, within BT4146 at 5444.403 kb on - strand, within BT4146 at 5444.405 kb on - strand, within BT4146 at 5444.491 kb on + strand, within BT4146 at 5444.609 kb on - strand, within BT4146 at 5444.618 kb on - strand, within BT4146 at 5444.645 kb on + strand, within BT4146 at 5444.832 kb on + strand, within BT4146 at 5444.945 kb on + strand, within BT4146 at 5444.990 kb on + strand, within BT4146 at 5445.020 kb on + strand, within BT4146 at 5445.353 kb on - strand, within BT4146 at 5445.401 kb on + strand, within BT4146 at 5445.502 kb on + strand at 5445.504 kb on + strand at 5445.551 kb on + strand at 5445.556 kb on - strand at 5445.645 kb on - strand at 5445.648 kb on - strand at 5445.676 kb on - strand at 5445.717 kb on - strand, within BT4147 at 5445.955 kb on + strand, within BT4147 at 5445.971 kb on - strand, within BT4147 at 5445.972 kb on + strand, within BT4147 at 5446.014 kb on + strand, within BT4147 at 5446.014 kb on + strand, within BT4147 at 5446.150 kb on - strand, within BT4147 at 5446.333 kb on + strand, within BT4148 at 5446.333 kb on + strand, within BT4148 at 5446.345 kb on + strand, within BT4148 at 5446.418 kb on - strand at 5446.452 kb on - strand at 5446.479 kb on + strand at 5446.481 kb on + strand at 5446.482 kb on - strand at 5446.482 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 5,443,346 + BT4145 0.70 -2.7 5,443,411 + BT4145 0.73 -0.9 5,443,448 + BT4145 0.74 +0.0 5,443,469 + BT4145 0.75 -0.2 5,443,518 + BT4145 0.77 +0.0 5,443,523 - BT4145 0.77 +1.6 5,443,576 + BT4145 0.79 -1.0 5,443,576 + BT4145 0.79 +0.3 5,443,576 + BT4145 0.79 -1.8 5,443,646 + BT4145 0.81 -1.6 5,443,646 + BT4145 0.81 -2.9 5,443,646 + BT4145 0.81 -1.8 5,443,769 - BT4145 0.86 +0.0 5,443,954 - -1.0 5,443,954 - -1.1 5,443,960 + -2.8 5,444,002 + -2.7 5,444,003 - +0.0 5,444,018 - -1.6 5,444,145 + -1.7 5,444,146 - -3.7 5,444,180 - -1.0 5,444,187 + -2.7 5,444,187 + -2.0 5,444,270 + -2.3 5,444,292 + +1.6 5,444,292 + -2.0 5,444,398 - BT4146 0.17 -1.6 5,444,400 + BT4146 0.17 +0.8 5,444,400 + BT4146 0.17 +1.0 5,444,400 + BT4146 0.17 -1.6 5,444,401 - BT4146 0.17 -1.0 5,444,403 - BT4146 0.17 -2.0 5,444,405 - BT4146 0.17 +3.0 5,444,491 + BT4146 0.23 -2.0 5,444,609 - BT4146 0.32 +1.0 5,444,618 - BT4146 0.32 -1.7 5,444,645 + BT4146 0.34 -2.8 5,444,832 + BT4146 0.48 -2.0 5,444,945 + BT4146 0.56 -2.1 5,444,990 + BT4146 0.59 -1.3 5,445,020 + BT4146 0.61 -2.6 5,445,353 - BT4146 0.85 -2.6 5,445,401 + BT4146 0.88 +2.3 5,445,502 + -2.2 5,445,504 + -3.1 5,445,551 + -2.3 5,445,556 - +0.0 5,445,645 - -0.9 5,445,648 - +1.5 5,445,676 - -1.7 5,445,717 - BT4147 0.10 -2.4 5,445,955 + BT4147 0.50 -2.3 5,445,971 - BT4147 0.53 -0.6 5,445,972 + BT4147 0.53 -2.3 5,446,014 + BT4147 0.60 +3.5 5,446,014 + BT4147 0.60 -1.0 5,446,150 - BT4147 0.83 -0.6 5,446,333 + BT4148 0.62 -1.0 5,446,333 + BT4148 0.62 +0.2 5,446,345 + BT4148 0.68 -0.7 5,446,418 - -1.6 5,446,452 - -0.7 5,446,479 + +0.0 5,446,481 + -1.0 5,446,482 - -1.6 5,446,482 - -0.7
Or see this region's nucleotide sequence