Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3982 and BT3983 are separated by 200 nucleotides BT3983 and BT3984 are separated by 16 nucleotides
BT3982: BT3982 - ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member (NCBI ptt file), at 5,183,985 to 5,185,403
BT3982
BT3983: BT3983 - putative outer membrane protein, probably involved in nutrient binding (NCBI ptt file), at 5,185,604 to 5,189,011
BT3983
BT3984: BT3984 - hypothetical protein (NCBI ptt file), at 5,189,028 to 5,190,641
BT3984
Position (kb) 5185
5186
5187
5188
5189
5190 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 5184.667 kb on - strand, within BT3982 at 5184.706 kb on + strand, within BT3982 at 5184.707 kb on - strand, within BT3982 at 5184.753 kb on + strand, within BT3982 at 5184.754 kb on - strand, within BT3982 at 5184.754 kb on - strand, within BT3982 at 5184.754 kb on - strand, within BT3982 at 5184.797 kb on + strand, within BT3982 at 5184.798 kb on - strand, within BT3982 at 5184.816 kb on + strand, within BT3982 at 5184.819 kb on - strand, within BT3982 at 5184.819 kb on - strand, within BT3982 at 5184.829 kb on + strand, within BT3982 at 5184.876 kb on - strand, within BT3982 at 5184.876 kb on - strand, within BT3982 at 5184.876 kb on - strand, within BT3982 at 5184.876 kb on - strand, within BT3982 at 5184.903 kb on + strand, within BT3982 at 5184.903 kb on + strand, within BT3982 at 5184.918 kb on + strand, within BT3982 at 5184.952 kb on + strand, within BT3982 at 5184.952 kb on + strand, within BT3982 at 5185.106 kb on - strand, within BT3982 at 5185.109 kb on + strand, within BT3982 at 5185.127 kb on + strand, within BT3982 at 5185.127 kb on + strand, within BT3982 at 5185.132 kb on + strand, within BT3982 at 5185.133 kb on - strand, within BT3982 at 5185.133 kb on - strand, within BT3982 at 5185.151 kb on + strand, within BT3982 at 5185.151 kb on + strand, within BT3982 at 5185.153 kb on + strand, within BT3982 at 5185.153 kb on + strand, within BT3982 at 5185.154 kb on - strand, within BT3982 at 5185.154 kb on - strand, within BT3982 at 5185.200 kb on + strand, within BT3982 at 5185.201 kb on - strand, within BT3982 at 5185.221 kb on + strand, within BT3982 at 5185.221 kb on + strand, within BT3982 at 5185.222 kb on - strand, within BT3982 at 5185.244 kb on + strand, within BT3982 at 5185.244 kb on - strand, within BT3982 at 5185.291 kb on - strand at 5185.291 kb on - strand at 5185.292 kb on + strand at 5185.293 kb on - strand at 5185.335 kb on + strand at 5185.336 kb on - strand at 5185.336 kb on - strand at 5185.369 kb on - strand at 5185.369 kb on - strand at 5185.446 kb on - strand at 5185.517 kb on - strand at 5185.543 kb on - strand at 5185.546 kb on + strand at 5185.547 kb on - strand at 5185.592 kb on - strand at 5185.726 kb on - strand at 5185.791 kb on - strand at 5185.830 kb on + strand at 5185.907 kb on - strand at 5186.001 kb on + strand, within BT3983 at 5186.002 kb on - strand, within BT3983 at 5186.012 kb on - strand, within BT3983 at 5186.065 kb on + strand, within BT3983 at 5186.065 kb on + strand, within BT3983 at 5186.066 kb on - strand, within BT3983 at 5186.088 kb on - strand, within BT3983 at 5186.088 kb on - strand, within BT3983 at 5186.090 kb on - strand, within BT3983 at 5186.102 kb on - strand, within BT3983 at 5186.133 kb on - strand, within BT3983 at 5186.271 kb on - strand, within BT3983 at 5186.271 kb on - strand, within BT3983 at 5186.359 kb on + strand, within BT3983 at 5186.362 kb on + strand, within BT3983 at 5186.395 kb on - strand, within BT3983 at 5186.395 kb on - strand, within BT3983 at 5186.395 kb on - strand, within BT3983 at 5186.398 kb on + strand, within BT3983 at 5186.403 kb on + strand, within BT3983 at 5186.450 kb on + strand, within BT3983 at 5186.488 kb on + strand, within BT3983 at 5186.524 kb on + strand, within BT3983 at 5186.546 kb on - strand at 5186.639 kb on - strand, within BT3983 at 5186.645 kb on - strand, within BT3983 at 5186.672 kb on - strand, within BT3983 at 5186.687 kb on - strand, within BT3983 at 5186.747 kb on - strand, within BT3983 at 5186.747 kb on - strand, within BT3983 at 5186.768 kb on - strand, within BT3983 at 5186.838 kb on - strand, within BT3983 at 5186.856 kb on + strand, within BT3983 at 5186.860 kb on + strand, within BT3983 at 5186.907 kb on - strand, within BT3983 at 5186.948 kb on - strand, within BT3983 at 5186.958 kb on - strand, within BT3983 at 5186.980 kb on - strand, within BT3983 at 5186.987 kb on - strand, within BT3983 at 5187.008 kb on - strand, within BT3983 at 5187.035 kb on + strand, within BT3983 at 5187.036 kb on - strand, within BT3983 at 5187.058 kb on + strand, within BT3983 at 5187.101 kb on + strand, within BT3983 at 5187.152 kb on + strand, within BT3983 at 5187.166 kb on + strand, within BT3983 at 5187.167 kb on - strand, within BT3983 at 5187.174 kb on - strand, within BT3983 at 5187.206 kb on - strand, within BT3983 at 5187.206 kb on - strand, within BT3983 at 5187.241 kb on + strand, within BT3983 at 5187.269 kb on + strand, within BT3983 at 5187.272 kb on - strand, within BT3983 at 5187.277 kb on - strand, within BT3983 at 5187.358 kb on + strand, within BT3983 at 5187.386 kb on - strand, within BT3983 at 5187.386 kb on - strand, within BT3983 at 5187.453 kb on - strand, within BT3983 at 5187.468 kb on + strand, within BT3983 at 5187.505 kb on - strand, within BT3983 at 5187.512 kb on - strand, within BT3983 at 5187.536 kb on - strand, within BT3983 at 5187.627 kb on - strand, within BT3983 at 5187.627 kb on - strand, within BT3983 at 5187.732 kb on - strand, within BT3983 at 5187.732 kb on - strand, within BT3983 at 5187.823 kb on + strand, within BT3983 at 5187.823 kb on + strand, within BT3983 at 5187.823 kb on + strand, within BT3983 at 5187.824 kb on - strand, within BT3983 at 5187.831 kb on + strand, within BT3983 at 5187.831 kb on + strand, within BT3983 at 5187.832 kb on - strand, within BT3983 at 5187.838 kb on - strand, within BT3983 at 5187.863 kb on - strand, within BT3983 at 5187.913 kb on + strand, within BT3983 at 5187.931 kb on + strand, within BT3983 at 5187.931 kb on + strand, within BT3983 at 5187.932 kb on - strand, within BT3983 at 5187.934 kb on - strand, within BT3983 at 5187.934 kb on - strand, within BT3983 at 5187.938 kb on - strand, within BT3983 at 5187.958 kb on + strand, within BT3983 at 5187.959 kb on - strand, within BT3983 at 5187.999 kb on + strand, within BT3983 at 5188.000 kb on - strand, within BT3983 at 5188.001 kb on + strand, within BT3983 at 5188.001 kb on + strand, within BT3983 at 5188.043 kb on - strand, within BT3983 at 5188.043 kb on - strand, within BT3983 at 5188.061 kb on - strand, within BT3983 at 5188.089 kb on - strand, within BT3983 at 5188.089 kb on - strand, within BT3983 at 5188.135 kb on - strand, within BT3983 at 5188.143 kb on - strand, within BT3983 at 5188.151 kb on - strand, within BT3983 at 5188.155 kb on - strand, within BT3983 at 5188.155 kb on - strand, within BT3983 at 5188.214 kb on - strand, within BT3983 at 5188.246 kb on + strand, within BT3983 at 5188.247 kb on - strand, within BT3983 at 5188.247 kb on - strand, within BT3983 at 5188.268 kb on - strand, within BT3983 at 5188.324 kb on + strand, within BT3983 at 5188.467 kb on + strand, within BT3983 at 5188.467 kb on + strand, within BT3983 at 5188.468 kb on - strand, within BT3983 at 5188.468 kb on - strand, within BT3983 at 5188.490 kb on - strand, within BT3983 at 5188.503 kb on + strand, within BT3983 at 5188.512 kb on + strand, within BT3983 at 5188.605 kb on + strand, within BT3983 at 5188.606 kb on - strand, within BT3983 at 5188.679 kb on + strand at 5188.679 kb on + strand at 5188.680 kb on - strand at 5188.690 kb on - strand at 5188.707 kb on + strand at 5188.708 kb on - strand at 5188.731 kb on + strand at 5188.781 kb on - strand at 5188.814 kb on - strand at 5188.814 kb on - strand at 5188.961 kb on - strand at 5188.982 kb on - strand at 5189.024 kb on - strand at 5189.036 kb on + strand at 5189.152 kb on - strand at 5189.363 kb on - strand, within BT3984 at 5189.453 kb on + strand, within BT3984 at 5189.453 kb on + strand, within BT3984 at 5189.556 kb on - strand, within BT3984 at 5189.583 kb on - strand, within BT3984 at 5189.635 kb on - strand, within BT3984 at 5189.636 kb on + strand, within BT3984 at 5189.638 kb on + strand, within BT3984 at 5189.638 kb on + strand, within BT3984 at 5189.642 kb on + strand, within BT3984 at 5189.643 kb on - strand, within BT3984 at 5189.654 kb on - strand, within BT3984 at 5189.654 kb on - strand, within BT3984 at 5189.718 kb on - strand, within BT3984 at 5189.838 kb on + strand, within BT3984 at 5189.838 kb on + strand, within BT3984 at 5189.838 kb on + strand, within BT3984 at 5189.838 kb on + strand, within BT3984 at 5189.864 kb on - strand, within BT3984 at 5189.940 kb on - strand, within BT3984 at 5190.006 kb on + strand, within BT3984 at 5190.006 kb on + strand, within BT3984 at 5190.007 kb on - strand, within BT3984
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 5,184,667 - BT3982 0.48 -1.2 5,184,706 + BT3982 0.51 +0.9 5,184,707 - BT3982 0.51 -2.1 5,184,753 + BT3982 0.54 +1.1 5,184,754 - BT3982 0.54 +1.1 5,184,754 - BT3982 0.54 -2.1 5,184,754 - BT3982 0.54 -1.3 5,184,797 + BT3982 0.57 -2.3 5,184,798 - BT3982 0.57 -2.3 5,184,816 + BT3982 0.59 +0.5 5,184,819 - BT3982 0.59 -1.1 5,184,819 - BT3982 0.59 -1.4 5,184,829 + BT3982 0.59 -1.9 5,184,876 - BT3982 0.63 +3.9 5,184,876 - BT3982 0.63 -1.1 5,184,876 - BT3982 0.63 -2.5 5,184,876 - BT3982 0.63 -1.6 5,184,903 + BT3982 0.65 -2.1 5,184,903 + BT3982 0.65 -1.1 5,184,918 + BT3982 0.66 -1.4 5,184,952 + BT3982 0.68 +0.5 5,184,952 + BT3982 0.68 -1.0 5,185,106 - BT3982 0.79 +2.5 5,185,109 + BT3982 0.79 -3.4 5,185,127 + BT3982 0.80 -2.1 5,185,127 + BT3982 0.80 +2.5 5,185,132 + BT3982 0.81 -1.6 5,185,133 - BT3982 0.81 -1.6 5,185,133 - BT3982 0.81 -1.7 5,185,151 + BT3982 0.82 -2.1 5,185,151 + BT3982 0.82 -1.1 5,185,153 + BT3982 0.82 -0.8 5,185,153 + BT3982 0.82 -0.5 5,185,154 - BT3982 0.82 -3.1 5,185,154 - BT3982 0.82 +0.9 5,185,200 + BT3982 0.86 -2.3 5,185,201 - BT3982 0.86 -3.1 5,185,221 + BT3982 0.87 -1.1 5,185,221 + BT3982 0.87 -3.5 5,185,222 - BT3982 0.87 -1.4 5,185,244 + BT3982 0.89 -1.3 5,185,244 - BT3982 0.89 +0.9 5,185,291 - -1.6 5,185,291 - -1.5 5,185,292 + -1.1 5,185,293 - -2.1 5,185,335 + -1.1 5,185,336 - -3.1 5,185,336 - +0.9 5,185,369 - +0.9 5,185,369 - -2.4 5,185,446 - -1.1 5,185,517 - -1.7 5,185,543 - -1.1 5,185,546 + -1.2 5,185,547 - -1.9 5,185,592 - -0.5 5,185,726 - +2.3 5,185,791 - +3.1 5,185,830 + -1.1 5,185,907 - -2.8 5,186,001 + BT3983 0.12 -1.1 5,186,002 - BT3983 0.12 -0.6 5,186,012 - BT3983 0.12 -0.1 5,186,065 + BT3983 0.14 -0.4 5,186,065 + BT3983 0.14 -1.9 5,186,066 - BT3983 0.14 -2.4 5,186,088 - BT3983 0.14 -1.8 5,186,088 - BT3983 0.14 -2.0 5,186,090 - BT3983 0.14 -1.4 5,186,102 - BT3983 0.15 -2.9 5,186,133 - BT3983 0.16 -2.5 5,186,271 - BT3983 0.20 -2.3 5,186,271 - BT3983 0.20 -2.6 5,186,359 + BT3983 0.22 -1.6 5,186,362 + BT3983 0.22 -0.5 5,186,395 - BT3983 0.23 -1.9 5,186,395 - BT3983 0.23 -2.9 5,186,395 - BT3983 0.23 -1.1 5,186,398 + BT3983 0.23 -0.8 5,186,403 + BT3983 0.23 -2.1 5,186,450 + BT3983 0.25 -0.6 5,186,488 + BT3983 0.26 -0.1 5,186,524 + BT3983 0.27 -2.4 5,186,546 - -1.1 5,186,639 - BT3983 0.30 +0.0 5,186,645 - BT3983 0.31 -2.4 5,186,672 - BT3983 0.31 -2.5 5,186,687 - BT3983 0.32 -2.6 5,186,747 - BT3983 0.34 -3.0 5,186,747 - BT3983 0.34 -2.6 5,186,768 - BT3983 0.34 -1.1 5,186,838 - BT3983 0.36 -1.4 5,186,856 + BT3983 0.37 -2.6 5,186,860 + BT3983 0.37 -1.1 5,186,907 - BT3983 0.38 -3.3 5,186,948 - BT3983 0.39 -4.0 5,186,958 - BT3983 0.40 -1.5 5,186,980 - BT3983 0.40 -2.4 5,186,987 - BT3983 0.41 -2.3 5,187,008 - BT3983 0.41 -1.1 5,187,035 + BT3983 0.42 -1.9 5,187,036 - BT3983 0.42 -2.9 5,187,058 + BT3983 0.43 -1.6 5,187,101 + BT3983 0.44 -2.4 5,187,152 + BT3983 0.45 -0.6 5,187,166 + BT3983 0.46 +0.4 5,187,167 - BT3983 0.46 -4.1 5,187,174 - BT3983 0.46 -0.8 5,187,206 - BT3983 0.47 -2.6 5,187,206 - BT3983 0.47 -0.9 5,187,241 + BT3983 0.48 +0.5 5,187,269 + BT3983 0.49 -1.1 5,187,272 - BT3983 0.49 -1.6 5,187,277 - BT3983 0.49 -0.6 5,187,358 + BT3983 0.51 -0.1 5,187,386 - BT3983 0.52 -3.2 5,187,386 - BT3983 0.52 -1.2 5,187,453 - BT3983 0.54 -2.1 5,187,468 + BT3983 0.55 +0.9 5,187,505 - BT3983 0.56 +1.4 5,187,512 - BT3983 0.56 -2.4 5,187,536 - BT3983 0.57 -2.3 5,187,627 - BT3983 0.59 -2.6 5,187,627 - BT3983 0.59 -2.4 5,187,732 - BT3983 0.62 -3.2 5,187,732 - BT3983 0.62 +4.1 5,187,823 + BT3983 0.65 +0.9 5,187,823 + BT3983 0.65 -2.1 5,187,823 + BT3983 0.65 -1.6 5,187,824 - BT3983 0.65 -4.1 5,187,831 + BT3983 0.65 -0.8 5,187,831 + BT3983 0.65 -2.6 5,187,832 - BT3983 0.65 -2.4 5,187,838 - BT3983 0.66 -1.1 5,187,863 - BT3983 0.66 -1.4 5,187,913 + BT3983 0.68 -2.3 5,187,931 + BT3983 0.68 -3.2 5,187,931 + BT3983 0.68 -2.2 5,187,932 - BT3983 0.68 -2.8 5,187,934 - BT3983 0.68 -0.5 5,187,934 - BT3983 0.68 -0.1 5,187,938 - BT3983 0.68 -1.3 5,187,958 + BT3983 0.69 -2.1 5,187,959 - BT3983 0.69 -0.1 5,187,999 + BT3983 0.70 -2.1 5,188,000 - BT3983 0.70 -2.4 5,188,001 + BT3983 0.70 -1.1 5,188,001 + BT3983 0.70 -3.5 5,188,043 - BT3983 0.72 -3.2 5,188,043 - BT3983 0.72 -0.1 5,188,061 - BT3983 0.72 -1.1 5,188,089 - BT3983 0.73 -0.9 5,188,089 - BT3983 0.73 +0.9 5,188,135 - BT3983 0.74 -1.9 5,188,143 - BT3983 0.75 -3.9 5,188,151 - BT3983 0.75 -1.8 5,188,155 - BT3983 0.75 -1.1 5,188,155 - BT3983 0.75 -1.1 5,188,214 - BT3983 0.77 -0.1 5,188,246 + BT3983 0.78 -1.1 5,188,247 - BT3983 0.78 -1.8 5,188,247 - BT3983 0.78 -3.5 5,188,268 - BT3983 0.78 +0.9 5,188,324 + BT3983 0.80 -1.1 5,188,467 + BT3983 0.84 -0.1 5,188,467 + BT3983 0.84 -0.1 5,188,468 - BT3983 0.84 -2.9 5,188,468 - BT3983 0.84 -2.2 5,188,490 - BT3983 0.85 -3.4 5,188,503 + BT3983 0.85 -1.4 5,188,512 + BT3983 0.85 -0.6 5,188,605 + BT3983 0.88 -3.1 5,188,606 - BT3983 0.88 -0.6 5,188,679 + -1.6 5,188,679 + -3.1 5,188,680 - -1.4 5,188,690 - -2.4 5,188,707 + -3.9 5,188,708 - +1.7 5,188,731 + -0.1 5,188,781 - -0.1 5,188,814 - -1.4 5,188,814 - -0.1 5,188,961 - -1.6 5,188,982 - -1.6 5,189,024 - -0.6 5,189,036 + -0.4 5,189,152 - -2.9 5,189,363 - BT3984 0.21 -2.1 5,189,453 + BT3984 0.26 -1.1 5,189,453 + BT3984 0.26 -3.1 5,189,556 - BT3984 0.33 -1.0 5,189,583 - BT3984 0.34 -1.6 5,189,635 - BT3984 0.38 -3.1 5,189,636 + BT3984 0.38 -1.1 5,189,638 + BT3984 0.38 -0.7 5,189,638 + BT3984 0.38 -0.1 5,189,642 + BT3984 0.38 -2.4 5,189,643 - BT3984 0.38 -1.1 5,189,654 - BT3984 0.39 -2.4 5,189,654 - BT3984 0.39 -4.8 5,189,718 - BT3984 0.43 +0.5 5,189,838 + BT3984 0.50 -2.1 5,189,838 + BT3984 0.50 -1.1 5,189,838 + BT3984 0.50 -1.1 5,189,838 + BT3984 0.50 -0.1 5,189,864 - BT3984 0.52 +0.2 5,189,940 - BT3984 0.57 -1.3 5,190,006 + BT3984 0.61 -0.1 5,190,006 + BT3984 0.61 -0.9 5,190,007 - BT3984 0.61 -0.6
Or see this region's nucleotide sequence