Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3912 and BT3913 are separated by 102 nucleotides BT3913 and BT3914 are separated by 11 nucleotides
BT3912: BT3912 - conserved hypothetical protein (NCBI ptt file), at 5,086,708 to 5,087,580
BT3912
BT3913: BT3913 - hypothetical protein (NCBI ptt file), at 5,087,683 to 5,088,264
BT3913
BT3914: BT3914 - hypothetical protein (NCBI ptt file), at 5,088,276 to 5,089,178
BT3914
Position (kb)
5087
5088
5089 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 5086.898 kb on + strand, within BT3912 at 5087.040 kb on - strand, within BT3912 at 5087.126 kb on - strand, within BT3912 at 5087.317 kb on - strand, within BT3912 at 5087.338 kb on - strand, within BT3912 at 5087.344 kb on + strand, within BT3912 at 5087.345 kb on - strand, within BT3912 at 5087.345 kb on - strand, within BT3912 at 5087.346 kb on + strand, within BT3912 at 5087.348 kb on + strand, within BT3912 at 5087.348 kb on + strand, within BT3912 at 5087.480 kb on + strand, within BT3912 at 5087.480 kb on + strand, within BT3912 at 5087.507 kb on - strand at 5087.676 kb on + strand at 5087.681 kb on + strand at 5087.682 kb on - strand at 5087.712 kb on - strand at 5087.714 kb on + strand at 5087.715 kb on - strand at 5087.717 kb on + strand at 5087.718 kb on - strand at 5087.813 kb on + strand, within BT3913 at 5087.815 kb on + strand, within BT3913 at 5087.816 kb on - strand, within BT3913 at 5087.860 kb on - strand, within BT3913 at 5087.882 kb on - strand, within BT3913 at 5087.883 kb on + strand, within BT3913 at 5087.883 kb on + strand, within BT3913 at 5087.884 kb on - strand, within BT3913 at 5087.963 kb on + strand, within BT3913 at 5088.056 kb on - strand, within BT3913 at 5088.098 kb on - strand, within BT3913 at 5088.098 kb on - strand, within BT3913 at 5088.099 kb on + strand, within BT3913 at 5088.117 kb on + strand, within BT3913 at 5088.118 kb on - strand, within BT3913 at 5088.118 kb on - strand, within BT3913 at 5088.146 kb on + strand, within BT3913 at 5088.146 kb on + strand, within BT3913 at 5088.148 kb on + strand, within BT3913 at 5088.149 kb on - strand, within BT3913 at 5088.149 kb on - strand, within BT3913 at 5088.149 kb on - strand, within BT3913 at 5088.149 kb on - strand, within BT3913 at 5088.200 kb on + strand, within BT3913 at 5088.292 kb on + strand at 5088.295 kb on + strand at 5088.295 kb on + strand at 5088.388 kb on + strand, within BT3914 at 5088.395 kb on - strand, within BT3914 at 5088.451 kb on - strand, within BT3914 at 5088.488 kb on + strand, within BT3914 at 5088.489 kb on - strand, within BT3914 at 5088.535 kb on - strand, within BT3914 at 5088.536 kb on + strand, within BT3914 at 5088.537 kb on - strand, within BT3914 at 5088.570 kb on - strand, within BT3914 at 5088.570 kb on - strand, within BT3914 at 5088.616 kb on - strand, within BT3914 at 5088.718 kb on - strand, within BT3914 at 5088.718 kb on - strand, within BT3914 at 5088.754 kb on - strand, within BT3914 at 5088.799 kb on + strand, within BT3914 at 5088.808 kb on + strand, within BT3914 at 5088.808 kb on + strand at 5088.809 kb on - strand, within BT3914 at 5088.814 kb on - strand, within BT3914 at 5088.815 kb on + strand, within BT3914 at 5088.816 kb on - strand, within BT3914 at 5088.837 kb on + strand, within BT3914 at 5088.838 kb on - strand, within BT3914 at 5088.838 kb on - strand, within BT3914 at 5088.893 kb on + strand, within BT3914 at 5088.894 kb on - strand, within BT3914 at 5088.917 kb on - strand, within BT3914 at 5088.934 kb on - strand, within BT3914 at 5088.938 kb on - strand, within BT3914 at 5088.965 kb on + strand, within BT3914 at 5088.970 kb on - strand, within BT3914 at 5088.970 kb on - strand, within BT3914 at 5089.025 kb on + strand, within BT3914 at 5089.172 kb on + strand at 5089.184 kb on + strand at 5089.184 kb on + strand at 5089.185 kb on - strand at 5089.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 5,086,898 + BT3912 0.22 -0.1 5,087,040 - BT3912 0.38 +0.5 5,087,126 - BT3912 0.48 -1.1 5,087,317 - BT3912 0.70 -0.1 5,087,338 - BT3912 0.72 -1.3 5,087,344 + BT3912 0.73 -1.1 5,087,345 - BT3912 0.73 -1.6 5,087,345 - BT3912 0.73 -2.1 5,087,346 + BT3912 0.73 +0.7 5,087,348 + BT3912 0.73 -0.1 5,087,348 + BT3912 0.73 -2.6 5,087,480 + BT3912 0.88 -0.1 5,087,480 + BT3912 0.88 -0.1 5,087,507 - -1.6 5,087,676 + -0.1 5,087,681 + -3.9 5,087,682 - -1.1 5,087,712 - -1.6 5,087,714 + -2.9 5,087,715 - -1.8 5,087,717 + -1.6 5,087,718 - -4.4 5,087,813 + BT3913 0.22 -2.4 5,087,815 + BT3913 0.23 -4.6 5,087,816 - BT3913 0.23 +0.6 5,087,860 - BT3913 0.30 -2.4 5,087,882 - BT3913 0.34 -1.6 5,087,883 + BT3913 0.34 +0.7 5,087,883 + BT3913 0.34 -1.1 5,087,884 - BT3913 0.35 +2.5 5,087,963 + BT3913 0.48 +2.3 5,088,056 - BT3913 0.64 -2.1 5,088,098 - BT3913 0.71 -2.1 5,088,098 - BT3913 0.71 -1.6 5,088,099 + BT3913 0.71 -3.4 5,088,117 + BT3913 0.75 -1.1 5,088,118 - BT3913 0.75 +1.5 5,088,118 - BT3913 0.75 -0.8 5,088,146 + BT3913 0.80 +0.8 5,088,146 + BT3913 0.80 -0.7 5,088,148 + BT3913 0.80 +0.0 5,088,149 - BT3913 0.80 -1.1 5,088,149 - BT3913 0.80 -0.1 5,088,149 - BT3913 0.80 -0.1 5,088,149 - BT3913 0.80 -0.1 5,088,200 + BT3913 0.89 -1.1 5,088,292 + -1.1 5,088,295 + -3.2 5,088,295 + -1.5 5,088,388 + BT3914 0.12 -1.1 5,088,395 - BT3914 0.13 -1.4 5,088,451 - BT3914 0.19 -2.3 5,088,488 + BT3914 0.23 -0.1 5,088,489 - BT3914 0.24 -2.6 5,088,535 - BT3914 0.29 -1.1 5,088,536 + BT3914 0.29 -2.9 5,088,537 - BT3914 0.29 -0.1 5,088,570 - BT3914 0.33 +0.5 5,088,570 - BT3914 0.33 -1.0 5,088,616 - BT3914 0.38 +0.7 5,088,718 - BT3914 0.49 -3.4 5,088,718 - BT3914 0.49 -1.1 5,088,754 - BT3914 0.53 -2.4 5,088,799 + BT3914 0.58 -1.1 5,088,808 + BT3914 0.59 -2.9 5,088,808 + -1.1 5,088,809 - BT3914 0.59 -3.1 5,088,814 - BT3914 0.60 +0.9 5,088,815 + BT3914 0.60 -2.4 5,088,816 - BT3914 0.60 -3.1 5,088,837 + BT3914 0.62 -2.3 5,088,838 - BT3914 0.62 -1.1 5,088,838 - BT3914 0.62 -2.2 5,088,893 + BT3914 0.68 -1.1 5,088,894 - BT3914 0.68 -2.6 5,088,917 - BT3914 0.71 -1.1 5,088,934 - BT3914 0.73 -2.1 5,088,938 - BT3914 0.73 +1.5 5,088,965 + BT3914 0.76 -3.9 5,088,970 - BT3914 0.77 +1.5 5,088,970 - BT3914 0.77 -3.4 5,089,025 + BT3914 0.83 -1.2 5,089,172 + +0.9 5,089,184 + -3.4 5,089,184 + -2.6 5,089,185 - +0.3 5,089,228 + -0.1
Or see this region's nucleotide sequence