Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3529 and BT3530 are separated by 139 nucleotides BT3530 and BT3531 are separated by 58 nucleotides
BT3529: BT3529 - aldose 1-epimerase precursor (NCBI ptt file), at 4,563,352 to 4,564,485
BT3529
BT3530: BT3530 - putative alpha-1,2-mannosidase (NCBI ptt file), at 4,564,625 to 4,566,586
BT3530
BT3531: BT3531 - conserved hypothetical protein (NCBI ptt file), at 4,566,645 to 4,568,708
BT3531
Position (kb)
4564
4565
4566
4567 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5
6 at 4563.666 kb on + strand, within BT3529 at 4563.666 kb on + strand, within BT3529 at 4563.730 kb on + strand, within BT3529 at 4563.731 kb on - strand, within BT3529 at 4563.770 kb on - strand, within BT3529 at 4563.858 kb on + strand, within BT3529 at 4563.895 kb on - strand, within BT3529 at 4563.948 kb on + strand, within BT3529 at 4563.964 kb on + strand, within BT3529 at 4563.965 kb on - strand, within BT3529 at 4564.028 kb on - strand, within BT3529 at 4564.030 kb on + strand, within BT3529 at 4564.033 kb on - strand, within BT3529 at 4564.048 kb on - strand, within BT3529 at 4564.178 kb on + strand, within BT3529 at 4564.216 kb on - strand, within BT3529 at 4564.262 kb on + strand, within BT3529 at 4564.263 kb on - strand, within BT3529 at 4564.263 kb on - strand, within BT3529 at 4564.264 kb on + strand, within BT3529 at 4564.264 kb on + strand at 4564.270 kb on - strand, within BT3529 at 4564.290 kb on - strand, within BT3529 at 4564.291 kb on + strand, within BT3529 at 4564.422 kb on - strand at 4564.493 kb on + strand at 4564.495 kb on + strand at 4564.498 kb on + strand at 4564.500 kb on + strand at 4564.506 kb on + strand at 4564.507 kb on - strand at 4564.565 kb on - strand at 4564.595 kb on + strand at 4564.595 kb on + strand at 4564.595 kb on + strand at 4564.655 kb on + strand at 4564.655 kb on + strand at 4564.656 kb on - strand at 4564.698 kb on + strand at 4564.699 kb on - strand at 4564.699 kb on - strand at 4564.778 kb on + strand at 4564.834 kb on + strand, within BT3530 at 4564.942 kb on + strand, within BT3530 at 4564.942 kb on + strand, within BT3530 at 4564.942 kb on + strand, within BT3530 at 4564.951 kb on + strand, within BT3530 at 4564.997 kb on + strand, within BT3530 at 4565.000 kb on - strand, within BT3530 at 4565.062 kb on - strand, within BT3530 at 4565.062 kb on - strand, within BT3530 at 4565.062 kb on - strand, within BT3530 at 4565.066 kb on - strand, within BT3530 at 4565.066 kb on - strand, within BT3530 at 4565.108 kb on + strand, within BT3530 at 4565.145 kb on - strand, within BT3530 at 4565.153 kb on + strand, within BT3530 at 4565.164 kb on - strand, within BT3530 at 4565.218 kb on + strand, within BT3530 at 4565.420 kb on - strand, within BT3530 at 4565.420 kb on - strand, within BT3530 at 4565.438 kb on + strand, within BT3530 at 4565.439 kb on - strand, within BT3530 at 4565.477 kb on + strand, within BT3530 at 4565.501 kb on + strand, within BT3530 at 4565.501 kb on + strand, within BT3530 at 4565.502 kb on - strand, within BT3530 at 4565.531 kb on - strand, within BT3530 at 4565.672 kb on - strand, within BT3530 at 4565.767 kb on + strand, within BT3530 at 4565.828 kb on + strand, within BT3530 at 4566.109 kb on + strand, within BT3530 at 4566.110 kb on - strand, within BT3530 at 4566.110 kb on - strand, within BT3530 at 4566.110 kb on - strand, within BT3530 at 4566.187 kb on - strand, within BT3530 at 4566.189 kb on + strand, within BT3530 at 4566.189 kb on + strand, within BT3530 at 4566.221 kb on - strand, within BT3530 at 4566.221 kb on - strand, within BT3530 at 4566.224 kb on + strand, within BT3530 at 4566.245 kb on - strand, within BT3530 at 4566.290 kb on + strand, within BT3530 at 4566.292 kb on + strand, within BT3530 at 4566.292 kb on + strand, within BT3530 at 4566.360 kb on - strand, within BT3530 at 4566.418 kb on - strand at 4566.418 kb on - strand at 4566.445 kb on - strand at 4566.449 kb on - strand at 4566.533 kb on - strand at 4566.573 kb on - strand at 4566.574 kb on + strand at 4566.578 kb on + strand at 4566.623 kb on + strand at 4566.665 kb on + strand at 4566.703 kb on + strand at 4566.745 kb on + strand at 4566.827 kb on + strand at 4567.027 kb on - strand, within BT3531 at 4567.081 kb on + strand, within BT3531 at 4567.082 kb on - strand, within BT3531 at 4567.107 kb on + strand, within BT3531 at 4567.185 kb on + strand, within BT3531 at 4567.186 kb on - strand, within BT3531 at 4567.186 kb on - strand, within BT3531 at 4567.189 kb on - strand, within BT3531 at 4567.194 kb on + strand, within BT3531 at 4567.194 kb on + strand, within BT3531 at 4567.234 kb on - strand, within BT3531 at 4567.281 kb on + strand, within BT3531 at 4567.282 kb on - strand, within BT3531 at 4567.333 kb on - strand, within BT3531 at 4567.335 kb on - strand, within BT3531 at 4567.471 kb on - strand, within BT3531 at 4567.473 kb on + strand, within BT3531 at 4567.473 kb on + strand, within BT3531 at 4567.476 kb on - strand, within BT3531 at 4567.512 kb on + strand, within BT3531 at 4567.512 kb on + strand, within BT3531 at 4567.513 kb on - strand, within BT3531 at 4567.519 kb on + strand, within BT3531 at 4567.519 kb on + strand, within BT3531 at 4567.520 kb on - strand, within BT3531
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 4,563,666 + BT3529 0.28 -3.3 4,563,666 + BT3529 0.28 -0.4 4,563,730 + BT3529 0.33 -0.0 4,563,731 - BT3529 0.33 -1.6 4,563,770 - BT3529 0.37 -2.4 4,563,858 + BT3529 0.45 -0.0 4,563,895 - BT3529 0.48 -1.6 4,563,948 + BT3529 0.53 -1.6 4,563,964 + BT3529 0.54 -3.6 4,563,965 - BT3529 0.54 -1.0 4,564,028 - BT3529 0.60 -1.3 4,564,030 + BT3529 0.60 -0.0 4,564,033 - BT3529 0.60 -2.8 4,564,048 - BT3529 0.61 -1.4 4,564,178 + BT3529 0.73 +0.0 4,564,216 - BT3529 0.76 -1.0 4,564,262 + BT3529 0.80 -2.4 4,564,263 - BT3529 0.80 -3.0 4,564,263 - BT3529 0.80 +1.0 4,564,264 + BT3529 0.80 -1.4 4,564,264 + -1.6 4,564,270 - BT3529 0.81 -2.4 4,564,290 - BT3529 0.83 -2.6 4,564,291 + BT3529 0.83 -1.6 4,564,422 - -1.6 4,564,493 + -2.2 4,564,495 + -2.5 4,564,498 + -0.0 4,564,500 + -3.4 4,564,506 + -0.0 4,564,507 - -3.2 4,564,565 - -2.6 4,564,595 + -0.0 4,564,595 + -3.0 4,564,595 + -0.7 4,564,655 + +0.1 4,564,655 + -1.0 4,564,656 - -2.4 4,564,698 + -4.5 4,564,699 - -0.9 4,564,699 - -1.3 4,564,778 + +0.2 4,564,834 + BT3530 0.11 -2.4 4,564,942 + BT3530 0.16 -0.0 4,564,942 + BT3530 0.16 -0.0 4,564,942 + BT3530 0.16 -2.6 4,564,951 + BT3530 0.17 -1.8 4,564,997 + BT3530 0.19 -2.0 4,565,000 - BT3530 0.19 +1.0 4,565,062 - BT3530 0.22 -0.0 4,565,062 - BT3530 0.22 -1.0 4,565,062 - BT3530 0.22 -2.0 4,565,066 - BT3530 0.22 -0.0 4,565,066 - BT3530 0.22 -0.0 4,565,108 + BT3530 0.25 -0.6 4,565,145 - BT3530 0.27 -1.1 4,565,153 + BT3530 0.27 +1.0 4,565,164 - BT3530 0.27 -1.0 4,565,218 + BT3530 0.30 -1.6 4,565,420 - BT3530 0.41 -2.8 4,565,420 - BT3530 0.41 -1.4 4,565,438 + BT3530 0.41 -0.8 4,565,439 - BT3530 0.41 -0.0 4,565,477 + BT3530 0.43 -1.0 4,565,501 + BT3530 0.45 +1.6 4,565,501 + BT3530 0.45 -0.5 4,565,502 - BT3530 0.45 -3.0 4,565,531 - BT3530 0.46 -0.8 4,565,672 - BT3530 0.53 -1.6 4,565,767 + BT3530 0.58 +1.6 4,565,828 + BT3530 0.61 +1.0 4,566,109 + BT3530 0.76 -0.0 4,566,110 - BT3530 0.76 -1.0 4,566,110 - BT3530 0.76 -1.6 4,566,110 - BT3530 0.76 -3.1 4,566,187 - BT3530 0.80 -5.2 4,566,189 + BT3530 0.80 -0.8 4,566,189 + BT3530 0.80 +5.8 4,566,221 - BT3530 0.81 -2.6 4,566,221 - BT3530 0.81 -0.2 4,566,224 + BT3530 0.81 -1.2 4,566,245 - BT3530 0.83 -1.2 4,566,290 + BT3530 0.85 -0.0 4,566,292 + BT3530 0.85 +1.9 4,566,292 + BT3530 0.85 -2.4 4,566,360 - BT3530 0.88 -0.4 4,566,418 - -2.6 4,566,418 - -2.6 4,566,445 - -1.0 4,566,449 - +0.2 4,566,533 - -1.0 4,566,573 - +1.0 4,566,574 + -2.0 4,566,578 + +0.6 4,566,623 + +1.8 4,566,665 + -3.4 4,566,703 + -2.6 4,566,745 + -1.6 4,566,827 + -2.4 4,567,027 - BT3531 0.19 -2.5 4,567,081 + BT3531 0.21 -1.6 4,567,082 - BT3531 0.21 -0.5 4,567,107 + BT3531 0.22 +2.0 4,567,185 + BT3531 0.26 -1.5 4,567,186 - BT3531 0.26 -1.5 4,567,186 - BT3531 0.26 -1.4 4,567,189 - BT3531 0.26 -0.0 4,567,194 + BT3531 0.27 -1.9 4,567,194 + BT3531 0.27 -2.0 4,567,234 - BT3531 0.29 -3.0 4,567,281 + BT3531 0.31 -1.6 4,567,282 - BT3531 0.31 -1.6 4,567,333 - BT3531 0.33 -0.5 4,567,335 - BT3531 0.33 -1.8 4,567,471 - BT3531 0.40 -0.6 4,567,473 + BT3531 0.40 -0.3 4,567,473 + BT3531 0.40 -2.2 4,567,476 - BT3531 0.40 -2.8 4,567,512 + BT3531 0.42 -1.4 4,567,512 + BT3531 0.42 -1.9 4,567,513 - BT3531 0.42 -0.0 4,567,519 + BT3531 0.42 +1.0 4,567,519 + BT3531 0.42 -1.5 4,567,520 - BT3531 0.42 -2.8
Or see this region's nucleotide sequence