Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2454 and BT2455 are separated by 99 nucleotides BT2455 and BT2456 are separated by 2 nucleotides BT2456 and BT2457 are separated by 884 nucleotides
BT2454: BT2454 - hypothetical protein (NCBI ptt file), at 3,070,999 to 3,071,256
BT2454
BT2455: BT2455 - reverse transcriptase/maturase family protein (NCBI ptt file), at 3,071,356 to 3,072,045
BT2455
BT2456: BT2456 - putative reverse transcriptase/maturase (NCBI ptt file), at 3,072,048 to 3,072,536
BT2456
BT2457: BT2457 - putative purple acid phosphatase (NCBI ptt file), at 3,073,421 to 3,074,590
BT2457
Position (kb)
3072
3073 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3071.072 kb on + strand, within BT2454 at 3071.073 kb on - strand, within BT2454 at 3071.125 kb on - strand, within BT2454 at 3071.138 kb on - strand, within BT2454 at 3071.141 kb on + strand, within BT2454 at 3071.141 kb on + strand, within BT2454 at 3071.156 kb on + strand, within BT2454 at 3071.193 kb on + strand, within BT2454 at 3071.194 kb on - strand, within BT2454 at 3071.194 kb on - strand, within BT2454 at 3071.228 kb on - strand, within BT2454 at 3071.229 kb on + strand, within BT2454 at 3071.230 kb on - strand, within BT2454 at 3071.230 kb on - strand, within BT2454 at 3071.244 kb on - strand at 3071.255 kb on - strand at 3071.319 kb on + strand at 3071.343 kb on + strand at 3071.364 kb on + strand at 3071.364 kb on + strand at 3071.364 kb on + strand at 3071.434 kb on - strand, within BT2455 at 3071.434 kb on - strand, within BT2455 at 3071.439 kb on + strand, within BT2455 at 3071.439 kb on + strand, within BT2455 at 3071.439 kb on + strand, within BT2455 at 3071.439 kb on + strand, within BT2455 at 3071.456 kb on + strand, within BT2455 at 3071.513 kb on - strand, within BT2455 at 3071.539 kb on + strand, within BT2455 at 3071.540 kb on - strand, within BT2455 at 3071.548 kb on - strand, within BT2455 at 3071.548 kb on - strand, within BT2455 at 3071.571 kb on + strand, within BT2455 at 3071.574 kb on + strand, within BT2455 at 3071.574 kb on + strand, within BT2455 at 3071.574 kb on + strand, within BT2455 at 3071.583 kb on + strand, within BT2455 at 3071.592 kb on + strand, within BT2455 at 3071.599 kb on - strand, within BT2455 at 3071.599 kb on - strand, within BT2455 at 3071.678 kb on - strand, within BT2455 at 3071.690 kb on - strand, within BT2455 at 3071.690 kb on - strand, within BT2455 at 3071.722 kb on + strand, within BT2455 at 3071.722 kb on + strand, within BT2455 at 3071.746 kb on + strand, within BT2455 at 3071.838 kb on + strand, within BT2455 at 3071.839 kb on - strand, within BT2455 at 3071.858 kb on + strand, within BT2455 at 3071.858 kb on + strand, within BT2455 at 3071.866 kb on - strand, within BT2455 at 3071.870 kb on - strand, within BT2455 at 3071.887 kb on - strand, within BT2455 at 3071.892 kb on - strand, within BT2455 at 3071.899 kb on + strand, within BT2455 at 3071.899 kb on + strand, within BT2455 at 3071.900 kb on - strand, within BT2455 at 3071.901 kb on + strand, within BT2455 at 3071.927 kb on - strand, within BT2455 at 3071.939 kb on - strand, within BT2455 at 3071.941 kb on + strand, within BT2455 at 3071.942 kb on - strand, within BT2455 at 3071.966 kb on - strand, within BT2455 at 3071.992 kb on + strand at 3072.005 kb on + strand at 3072.006 kb on - strand at 3072.024 kb on + strand at 3072.171 kb on + strand, within BT2456 at 3072.171 kb on + strand, within BT2456 at 3072.171 kb on + strand, within BT2456 at 3072.203 kb on + strand, within BT2456 at 3072.203 kb on + strand, within BT2456 at 3072.204 kb on - strand, within BT2456 at 3072.204 kb on - strand, within BT2456 at 3072.204 kb on - strand, within BT2456 at 3072.207 kb on + strand, within BT2456 at 3072.208 kb on - strand, within BT2456 at 3072.233 kb on - strand, within BT2456 at 3072.233 kb on - strand, within BT2456 at 3072.258 kb on - strand, within BT2456 at 3072.258 kb on - strand, within BT2456 at 3072.311 kb on + strand, within BT2456 at 3072.311 kb on + strand, within BT2456 at 3072.312 kb on - strand, within BT2456 at 3072.312 kb on - strand, within BT2456 at 3072.312 kb on - strand, within BT2456 at 3072.348 kb on + strand, within BT2456 at 3072.348 kb on + strand at 3072.348 kb on + strand, within BT2456 at 3072.348 kb on + strand, within BT2456 at 3072.349 kb on - strand, within BT2456 at 3072.393 kb on + strand, within BT2456 at 3072.394 kb on - strand, within BT2456 at 3072.394 kb on - strand, within BT2456 at 3072.394 kb on - strand, within BT2456 at 3072.446 kb on + strand, within BT2456 at 3072.446 kb on + strand, within BT2456 at 3072.448 kb on + strand, within BT2456 at 3072.448 kb on + strand, within BT2456 at 3072.449 kb on - strand, within BT2456 at 3072.526 kb on - strand at 3072.558 kb on + strand at 3072.568 kb on + strand at 3072.568 kb on + strand at 3072.569 kb on - strand at 3072.569 kb on - strand at 3072.573 kb on - strand at 3072.604 kb on + strand at 3072.604 kb on + strand at 3072.612 kb on - strand at 3072.662 kb on + strand at 3072.663 kb on - strand at 3072.706 kb on + strand at 3072.723 kb on - strand at 3072.725 kb on - strand at 3072.737 kb on + strand at 3072.755 kb on + strand at 3072.755 kb on + strand at 3072.756 kb on - strand at 3072.759 kb on + strand at 3072.760 kb on - strand at 3072.760 kb on - strand at 3072.760 kb on - strand at 3072.787 kb on + strand at 3072.799 kb on + strand at 3072.800 kb on - strand at 3072.800 kb on - strand at 3072.806 kb on + strand at 3072.809 kb on + strand at 3072.856 kb on + strand at 3072.871 kb on + strand at 3072.909 kb on + strand at 3072.928 kb on + strand at 3072.929 kb on - strand at 3072.929 kb on - strand at 3072.937 kb on + strand at 3072.943 kb on - strand at 3073.014 kb on + strand at 3073.108 kb on + strand at 3073.108 kb on + strand at 3073.108 kb on + strand at 3073.109 kb on - strand at 3073.109 kb on - strand at 3073.135 kb on + strand at 3073.142 kb on - strand at 3073.252 kb on - strand at 3073.252 kb on - strand at 3073.272 kb on - strand at 3073.289 kb on + strand at 3073.333 kb on - strand at 3073.333 kb on - strand at 3073.367 kb on + strand at 3073.407 kb on + strand at 3073.408 kb on - strand at 3073.408 kb on - strand at 3073.408 kb on - strand at 3073.430 kb on + strand at 3073.475 kb on + strand at 3073.504 kb on + strand at 3073.505 kb on - strand at 3073.520 kb on - strand at 3073.529 kb on - strand at 3073.529 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 3,071,072 + BT2454 0.28 -2.0 3,071,073 - BT2454 0.29 -1.0 3,071,125 - BT2454 0.49 -1.3 3,071,138 - BT2454 0.54 -2.8 3,071,141 + BT2454 0.55 -1.6 3,071,141 + BT2454 0.55 +0.0 3,071,156 + BT2454 0.61 -2.7 3,071,193 + BT2454 0.75 +0.0 3,071,194 - BT2454 0.76 +1.0 3,071,194 - BT2454 0.76 -2.1 3,071,228 - BT2454 0.89 -2.8 3,071,229 + BT2454 0.89 -1.3 3,071,230 - BT2454 0.90 -1.3 3,071,230 - BT2454 0.90 +0.0 3,071,244 - -4.1 3,071,255 - -2.0 3,071,319 + -3.3 3,071,343 + -1.0 3,071,364 + -0.5 3,071,364 + -4.2 3,071,364 + -2.6 3,071,434 - BT2455 0.11 +0.4 3,071,434 - BT2455 0.11 -3.2 3,071,439 + BT2455 0.12 -1.7 3,071,439 + BT2455 0.12 -1.0 3,071,439 + BT2455 0.12 -1.6 3,071,439 + BT2455 0.12 +0.2 3,071,456 + BT2455 0.14 -1.4 3,071,513 - BT2455 0.23 -1.0 3,071,539 + BT2455 0.27 -1.9 3,071,540 - BT2455 0.27 -3.0 3,071,548 - BT2455 0.28 -1.0 3,071,548 - BT2455 0.28 -1.1 3,071,571 + BT2455 0.31 +0.8 3,071,574 + BT2455 0.32 -1.6 3,071,574 + BT2455 0.32 -2.6 3,071,574 + BT2455 0.32 -1.0 3,071,583 + BT2455 0.33 -3.2 3,071,592 + BT2455 0.34 +3.2 3,071,599 - BT2455 0.35 -0.6 3,071,599 - BT2455 0.35 -1.4 3,071,678 - BT2455 0.47 -1.2 3,071,690 - BT2455 0.48 +0.0 3,071,690 - BT2455 0.48 -2.3 3,071,722 + BT2455 0.53 -2.9 3,071,722 + BT2455 0.53 -1.0 3,071,746 + BT2455 0.57 -2.2 3,071,838 + BT2455 0.70 -1.7 3,071,839 - BT2455 0.70 -2.1 3,071,858 + BT2455 0.73 -1.6 3,071,858 + BT2455 0.73 +0.0 3,071,866 - BT2455 0.74 -2.4 3,071,870 - BT2455 0.74 +0.0 3,071,887 - BT2455 0.77 -3.2 3,071,892 - BT2455 0.78 +0.8 3,071,899 + BT2455 0.79 +0.0 3,071,899 + BT2455 0.79 -2.0 3,071,900 - BT2455 0.79 -0.6 3,071,901 + BT2455 0.79 -2.3 3,071,927 - BT2455 0.83 -0.4 3,071,939 - BT2455 0.84 -1.8 3,071,941 + BT2455 0.85 -0.2 3,071,942 - BT2455 0.85 -1.9 3,071,966 - BT2455 0.88 -2.2 3,071,992 + -0.7 3,072,005 + +0.6 3,072,006 - -1.0 3,072,024 + -1.5 3,072,171 + BT2456 0.25 +1.6 3,072,171 + BT2456 0.25 -1.0 3,072,171 + BT2456 0.25 -0.0 3,072,203 + BT2456 0.32 -1.6 3,072,203 + BT2456 0.32 -2.1 3,072,204 - BT2456 0.32 -3.2 3,072,204 - BT2456 0.32 -1.5 3,072,204 - BT2456 0.32 -3.7 3,072,207 + BT2456 0.33 -2.3 3,072,208 - BT2456 0.33 -2.3 3,072,233 - BT2456 0.38 -1.0 3,072,233 - BT2456 0.38 -2.7 3,072,258 - BT2456 0.43 -2.0 3,072,258 - BT2456 0.43 -2.6 3,072,311 + BT2456 0.54 -1.0 3,072,311 + BT2456 0.54 -2.7 3,072,312 - BT2456 0.54 -2.0 3,072,312 - BT2456 0.54 -0.0 3,072,312 - BT2456 0.54 -2.6 3,072,348 + BT2456 0.61 -0.0 3,072,348 + -0.0 3,072,348 + BT2456 0.61 -1.3 3,072,348 + BT2456 0.61 -0.8 3,072,349 - BT2456 0.62 -1.0 3,072,393 + BT2456 0.71 -3.0 3,072,394 - BT2456 0.71 -2.3 3,072,394 - BT2456 0.71 -1.0 3,072,394 - BT2456 0.71 -3.3 3,072,446 + BT2456 0.81 -2.3 3,072,446 + BT2456 0.81 -3.5 3,072,448 + BT2456 0.82 +0.4 3,072,448 + BT2456 0.82 -1.6 3,072,449 - BT2456 0.82 -1.3 3,072,526 - +0.6 3,072,558 + +0.6 3,072,568 + -0.0 3,072,568 + -3.3 3,072,569 - -2.0 3,072,569 - -2.0 3,072,573 - +3.0 3,072,604 + -2.8 3,072,604 + -0.0 3,072,612 - -1.4 3,072,662 + -3.7 3,072,663 - +1.0 3,072,706 + -2.8 3,072,723 - -1.3 3,072,725 - -3.4 3,072,737 + -0.7 3,072,755 + -1.6 3,072,755 + -0.0 3,072,756 - -0.3 3,072,759 + -0.0 3,072,760 - -0.0 3,072,760 - -1.6 3,072,760 - -2.0 3,072,787 + -4.0 3,072,799 + +1.6 3,072,800 - -3.5 3,072,800 - -1.6 3,072,806 + -1.7 3,072,809 + +1.4 3,072,856 + -2.7 3,072,871 + -3.0 3,072,909 + -3.0 3,072,928 + -2.9 3,072,929 - -1.0 3,072,929 - -3.2 3,072,937 + -2.0 3,072,943 - -0.0 3,073,014 + +1.0 3,073,108 + -2.3 3,073,108 + +1.1 3,073,108 + -2.3 3,073,109 - -2.6 3,073,109 - -1.2 3,073,135 + -2.7 3,073,142 - -3.0 3,073,252 - -1.1 3,073,252 - -1.3 3,073,272 - -2.0 3,073,289 + -1.0 3,073,333 - -1.5 3,073,333 - -1.6 3,073,367 + -1.4 3,073,407 + +1.7 3,073,408 - -2.4 3,073,408 - -0.0 3,073,408 - -0.4 3,073,430 + +0.6 3,073,475 + -0.0 3,073,504 + -2.6 3,073,505 - -0.6 3,073,520 - -1.6 3,073,529 - -0.0 3,073,529 - -1.9
Or see this region's nucleotide sequence