Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2020 and BT2021 are separated by 658 nucleotides BT2021 and BT2022 are separated by 227 nucleotides BT2022 and BT2023 overlap by 28 nucleotides
BT2020: BT2020 - putative phosphate/sulphate permeases (NCBI ptt file), at 2,534,972 to 2,537,224
BT2020
BT2021: BT2021 - conserved hypothetical protein, putative non-specific DNA-binding protein (NCBI ptt file), at 2,537,883 to 2,538,410
BT2021
BT2022: BT2022 - hypothetical protein (NCBI ptt file), at 2,538,638 to 2,539,081
BT2022
BT2023: BT2023 - two-component system sensor histidine kinase (NCBI ptt file), at 2,539,054 to 2,541,216
BT2023
Position (kb)
2537
2538
2539 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2536.961 kb on + strand, within BT2020 at 2536.962 kb on - strand, within BT2020 at 2537.115 kb on + strand at 2537.115 kb on + strand at 2537.120 kb on - strand at 2537.165 kb on + strand at 2537.166 kb on - strand at 2537.188 kb on - strand at 2537.278 kb on - strand at 2537.286 kb on - strand at 2537.290 kb on - strand at 2537.399 kb on - strand at 2537.407 kb on - strand at 2537.455 kb on + strand at 2537.456 kb on - strand at 2537.456 kb on - strand at 2537.460 kb on + strand at 2537.475 kb on + strand at 2537.476 kb on - strand at 2537.517 kb on + strand at 2537.566 kb on + strand at 2537.576 kb on + strand at 2537.580 kb on + strand at 2537.581 kb on - strand at 2537.584 kb on - strand at 2537.588 kb on + strand at 2537.595 kb on - strand at 2537.598 kb on + strand at 2537.638 kb on + strand at 2537.643 kb on + strand at 2537.644 kb on - strand at 2537.644 kb on - strand at 2537.703 kb on + strand at 2537.704 kb on - strand at 2537.792 kb on - strand at 2537.805 kb on - strand at 2537.837 kb on - strand at 2537.859 kb on + strand at 2538.021 kb on + strand, within BT2021 at 2538.193 kb on - strand, within BT2021 at 2538.347 kb on - strand, within BT2021 at 2538.349 kb on + strand, within BT2021 at 2538.350 kb on - strand, within BT2021 at 2538.388 kb on + strand at 2538.389 kb on - strand at 2538.389 kb on - strand at 2538.411 kb on - strand at 2538.411 kb on - strand at 2538.483 kb on + strand at 2538.508 kb on - strand at 2538.516 kb on + strand at 2538.516 kb on + strand at 2538.563 kb on + strand at 2538.564 kb on - strand at 2538.564 kb on - strand at 2538.585 kb on - strand at 2538.655 kb on - strand at 2538.656 kb on + strand at 2538.750 kb on + strand, within BT2022 at 2538.778 kb on - strand, within BT2022 at 2538.910 kb on + strand, within BT2022 at 2539.277 kb on + strand, within BT2023 at 2539.277 kb on + strand, within BT2023 at 2539.277 kb on + strand, within BT2023
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 2,536,961 + BT2020 0.88 -3.5 2,536,962 - BT2020 0.88 -1.6 2,537,115 + +0.0 2,537,115 + -1.2 2,537,120 - -0.6 2,537,165 + -1.8 2,537,166 - -2.0 2,537,188 - -1.0 2,537,278 - -0.5 2,537,286 - +0.0 2,537,290 - -3.2 2,537,399 - -1.8 2,537,407 - +0.0 2,537,455 + -2.4 2,537,456 - +0.0 2,537,456 - +1.0 2,537,460 + -1.8 2,537,475 + -2.3 2,537,476 - -2.0 2,537,517 + -0.7 2,537,566 + +1.0 2,537,576 + -2.0 2,537,580 + -2.4 2,537,581 - -2.0 2,537,584 - -2.0 2,537,588 + -3.3 2,537,595 - -2.0 2,537,598 + +0.2 2,537,638 + -2.6 2,537,643 + +1.0 2,537,644 - -2.3 2,537,644 - +0.0 2,537,703 + -2.3 2,537,704 - +0.3 2,537,792 - -1.4 2,537,805 - -1.6 2,537,837 - -2.6 2,537,859 + -2.0 2,538,021 + BT2021 0.26 -4.8 2,538,193 - BT2021 0.59 -2.3 2,538,347 - BT2021 0.88 +0.0 2,538,349 + BT2021 0.88 +0.0 2,538,350 - BT2021 0.88 -2.8 2,538,388 + -0.4 2,538,389 - -0.4 2,538,389 - -4.0 2,538,411 - -2.8 2,538,411 - -3.2 2,538,483 + -2.4 2,538,508 - -1.0 2,538,516 + -3.2 2,538,516 + +0.0 2,538,563 + -1.0 2,538,564 - -1.6 2,538,564 - -2.4 2,538,585 - -1.0 2,538,655 - +0.6 2,538,656 + -3.0 2,538,750 + BT2022 0.25 +1.6 2,538,778 - BT2022 0.32 +0.0 2,538,910 + BT2022 0.61 -3.2 2,539,277 + BT2023 0.10 -1.0 2,539,277 + BT2023 0.10 -1.6 2,539,277 + BT2023 0.10 -3.7
Or see this region's nucleotide sequence