Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0986 and BT0987 are separated by 123 nucleotides BT0987 and BT0988 are separated by 163 nucleotides
BT0986: BT0986 - conserved hypothetical protein, putative DNA-binding protein (NCBI ptt file), at 1,212,438 to 1,215,755
BT0986
BT0987: BT0987 - putative cytochrome C-type biogenesis protein (NCBI ptt file), at 1,215,879 to 1,217,393
BT0987
BT0988: BT0988 - Mg2+ transport ATPase protein B (NCBI ptt file), at 1,217,557 to 1,220,208
BT0988
Position (kb)
1215
1216
1217
1218 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1214.889 kb on - strand, within BT0986 at 1215.195 kb on - strand, within BT0986 at 1215.393 kb on - strand, within BT0986 at 1215.529 kb on + strand at 1215.530 kb on - strand at 1215.666 kb on + strand at 1215.667 kb on - strand at 1215.758 kb on + strand at 1215.777 kb on + strand at 1215.806 kb on - strand at 1215.815 kb on - strand at 1215.832 kb on + strand at 1215.909 kb on + strand at 1215.950 kb on + strand at 1215.976 kb on - strand at 1216.011 kb on - strand at 1216.088 kb on - strand, within BT0987 at 1216.276 kb on + strand, within BT0987 at 1216.307 kb on + strand, within BT0987 at 1216.341 kb on + strand, within BT0987 at 1216.342 kb on - strand, within BT0987 at 1216.391 kb on + strand, within BT0987 at 1216.394 kb on - strand, within BT0987 at 1216.488 kb on + strand, within BT0987 at 1216.509 kb on - strand, within BT0987 at 1216.513 kb on + strand, within BT0987 at 1216.520 kb on - strand, within BT0987 at 1216.548 kb on - strand, within BT0987 at 1216.608 kb on - strand, within BT0987 at 1216.608 kb on - strand, within BT0987 at 1216.637 kb on - strand, within BT0987 at 1216.653 kb on + strand, within BT0987 at 1216.665 kb on - strand, within BT0987 at 1216.665 kb on - strand, within BT0987 at 1216.668 kb on + strand, within BT0987 at 1216.760 kb on - strand, within BT0987 at 1216.832 kb on - strand, within BT0987 at 1216.842 kb on - strand, within BT0987 at 1216.918 kb on + strand, within BT0987 at 1216.919 kb on - strand, within BT0987 at 1216.926 kb on + strand, within BT0987 at 1216.928 kb on + strand, within BT0987 at 1216.933 kb on + strand, within BT0987 at 1217.034 kb on + strand, within BT0987 at 1217.055 kb on - strand, within BT0987 at 1217.057 kb on - strand, within BT0987 at 1217.094 kb on + strand, within BT0987 at 1217.168 kb on + strand, within BT0987 at 1217.377 kb on + strand at 1217.411 kb on - strand at 1217.424 kb on + strand at 1217.462 kb on - strand at 1217.521 kb on - strand at 1217.522 kb on + strand at 1217.522 kb on + strand at 1217.523 kb on - strand at 1217.729 kb on - strand at 1217.772 kb on + strand at 1217.794 kb on + strand at 1217.794 kb on + strand at 1217.794 kb on + strand at 1217.822 kb on - strand at 1217.910 kb on + strand, within BT0988 at 1217.910 kb on + strand, within BT0988 at 1217.935 kb on - strand, within BT0988 at 1218.042 kb on + strand, within BT0988 at 1218.042 kb on + strand, within BT0988 at 1218.047 kb on + strand, within BT0988 at 1218.053 kb on - strand, within BT0988 at 1218.160 kb on + strand, within BT0988 at 1218.162 kb on + strand, within BT0988 at 1218.168 kb on + strand, within BT0988 at 1218.169 kb on - strand, within BT0988 at 1218.289 kb on + strand, within BT0988 at 1218.355 kb on - strand, within BT0988 at 1218.382 kb on + strand, within BT0988 at 1218.382 kb on + strand, within BT0988
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 1,214,889 - BT0986 0.74 -1.8 1,215,195 - BT0986 0.83 -2.7 1,215,393 - BT0986 0.89 -0.1 1,215,529 + -2.1 1,215,530 - -0.7 1,215,666 + -1.4 1,215,667 - -1.1 1,215,758 + -0.1 1,215,777 + -1.1 1,215,806 - -0.1 1,215,815 - +0.1 1,215,832 + -0.1 1,215,909 + -1.1 1,215,950 + -2.3 1,215,976 - -0.1 1,216,011 - -2.1 1,216,088 - BT0987 0.14 +0.7 1,216,276 + BT0987 0.26 +2.6 1,216,307 + BT0987 0.28 -2.9 1,216,341 + BT0987 0.30 -0.7 1,216,342 - BT0987 0.31 -2.3 1,216,391 + BT0987 0.34 -1.1 1,216,394 - BT0987 0.34 -1.7 1,216,488 + BT0987 0.40 +1.9 1,216,509 - BT0987 0.42 +0.9 1,216,513 + BT0987 0.42 +0.3 1,216,520 - BT0987 0.42 -0.1 1,216,548 - BT0987 0.44 -0.1 1,216,608 - BT0987 0.48 -2.7 1,216,608 - BT0987 0.48 -0.1 1,216,637 - BT0987 0.50 -0.1 1,216,653 + BT0987 0.51 -0.1 1,216,665 - BT0987 0.52 -1.8 1,216,665 - BT0987 0.52 -1.3 1,216,668 + BT0987 0.52 -0.1 1,216,760 - BT0987 0.58 -0.1 1,216,832 - BT0987 0.63 +1.0 1,216,842 - BT0987 0.64 -1.7 1,216,918 + BT0987 0.69 -0.1 1,216,919 - BT0987 0.69 -0.1 1,216,926 + BT0987 0.69 -0.1 1,216,928 + BT0987 0.69 -0.5 1,216,933 + BT0987 0.70 -0.7 1,217,034 + BT0987 0.76 -1.7 1,217,055 - BT0987 0.78 -1.1 1,217,057 - BT0987 0.78 -1.7 1,217,094 + BT0987 0.80 -0.8 1,217,168 + BT0987 0.85 -1.1 1,217,377 + -0.1 1,217,411 - -2.4 1,217,424 + -1.7 1,217,462 - -0.1 1,217,521 - +0.9 1,217,522 + -1.7 1,217,522 + -1.1 1,217,523 - -1.1 1,217,729 - -0.7 1,217,772 + -1.0 1,217,794 + -3.1 1,217,794 + -2.1 1,217,794 + -1.1 1,217,822 - -0.1 1,217,910 + BT0988 0.13 +0.2 1,217,910 + BT0988 0.13 +0.9 1,217,935 - BT0988 0.14 -0.1 1,218,042 + BT0988 0.18 -0.1 1,218,042 + BT0988 0.18 -1.7 1,218,047 + BT0988 0.18 -1.7 1,218,053 - BT0988 0.19 +0.9 1,218,160 + BT0988 0.23 -2.9 1,218,162 + BT0988 0.23 -0.1 1,218,168 + BT0988 0.23 -1.7 1,218,169 - BT0988 0.23 +0.7 1,218,289 + BT0988 0.28 -1.1 1,218,355 - BT0988 0.30 +0.9 1,218,382 + BT0988 0.31 -1.6 1,218,382 + BT0988 0.31 -0.7
Or see this region's nucleotide sequence