Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0222 and BT0223 are separated by 70 nucleotides BT0223 and BT0224 are separated by 128 nucleotides BT0224 and BT0225 are separated by 41 nucleotides
BT0222: BT0222 - putative two-component system sensor kinase, but no kinase domain (NCBI ptt file), at 242,935 to 243,960
BT0222
BT0223: BT0223 - two-component system response regulator (NCBI ptt file), at 244,031 to 244,723
BT0223
BT0224: BT0224 - hypothetical protein (NCBI ptt file), at 244,852 to 245,256
BT0224
BT0225: BT0225 - hypothetical protein (NCBI ptt file), at 245,298 to 245,891
BT0225
Position (kb) 244
245 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 243.262 kb on - strand, within BT0222 at 243.267 kb on + strand, within BT0222 at 243.280 kb on - strand, within BT0222 at 243.282 kb on - strand, within BT0222 at 243.408 kb on + strand, within BT0222 at 243.409 kb on - strand, within BT0222 at 243.520 kb on + strand, within BT0222 at 243.553 kb on + strand, within BT0222 at 243.555 kb on + strand, within BT0222 at 243.558 kb on - strand, within BT0222 at 243.672 kb on + strand, within BT0222 at 243.673 kb on - strand, within BT0222 at 243.697 kb on - strand, within BT0222 at 243.697 kb on - strand, within BT0222 at 243.721 kb on - strand, within BT0222 at 243.800 kb on + strand, within BT0222 at 243.802 kb on + strand, within BT0222 at 243.802 kb on + strand, within BT0222 at 243.803 kb on - strand, within BT0222 at 243.803 kb on - strand, within BT0222 at 243.830 kb on + strand, within BT0222 at 243.831 kb on - strand, within BT0222 at 244.050 kb on + strand at 244.051 kb on - strand at 244.115 kb on + strand, within BT0223 at 244.116 kb on - strand, within BT0223 at 244.344 kb on + strand, within BT0223 at 244.446 kb on + strand, within BT0223 at 244.580 kb on + strand, within BT0223 at 244.594 kb on + strand, within BT0223 at 244.741 kb on + strand at 244.776 kb on + strand at 244.777 kb on - strand at 244.779 kb on - strand at 244.779 kb on - strand at 244.833 kb on + strand at 244.841 kb on - strand at 244.982 kb on - strand, within BT0224 at 244.982 kb on - strand, within BT0224 at 245.221 kb on + strand at 245.300 kb on - strand at 245.320 kb on - strand at 245.324 kb on - strand at 245.542 kb on - strand, within BT0225 at 245.542 kb on - strand, within BT0225 at 245.571 kb on + strand, within BT0225 at 245.695 kb on + strand, within BT0225
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 243,262 - BT0222 0.32 -3.6 243,267 + BT0222 0.32 -2.7 243,280 - BT0222 0.34 -1.3 243,282 - BT0222 0.34 -1.6 243,408 + BT0222 0.46 +0.3 243,409 - BT0222 0.46 +0.6 243,520 + BT0222 0.57 -1.0 243,553 + BT0222 0.60 -2.0 243,555 + BT0222 0.60 -2.6 243,558 - BT0222 0.61 +0.0 243,672 + BT0222 0.72 +1.0 243,673 - BT0222 0.72 -2.0 243,697 - BT0222 0.74 -1.6 243,697 - BT0222 0.74 -2.3 243,721 - BT0222 0.77 -1.6 243,800 + BT0222 0.84 -1.0 243,802 + BT0222 0.85 -3.4 243,802 + BT0222 0.85 -2.2 243,803 - BT0222 0.85 -1.6 243,803 - BT0222 0.85 -2.7 243,830 + BT0222 0.87 +2.8 243,831 - BT0222 0.87 +0.0 244,050 + -2.0 244,051 - -1.0 244,115 + BT0223 0.12 -3.3 244,116 - BT0223 0.12 -2.0 244,344 + BT0223 0.45 -2.6 244,446 + BT0223 0.60 -2.0 244,580 + BT0223 0.79 -1.6 244,594 + BT0223 0.81 -2.2 244,741 + -2.3 244,776 + -1.6 244,777 - -2.3 244,779 - -2.0 244,779 - +0.0 244,833 + +1.0 244,841 - -2.8 244,982 - BT0224 0.32 -1.0 244,982 - BT0224 0.32 +0.0 245,221 + +1.8 245,300 - -1.6 245,320 - +0.4 245,324 - -3.3 245,542 - BT0225 0.41 -1.0 245,542 - BT0225 0.41 -1.0 245,571 + BT0225 0.46 -2.6 245,695 + BT0225 0.67 +0.6
Or see this region's nucleotide sequence