Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0184 and BT0185 are separated by 101 nucleotides BT0185 and BT0186 are separated by 9 nucleotides BT0186 and BT0187 are separated by 95 nucleotides BT0187 and BT0188 are separated by 192 nucleotides
BT0184: BT0184 - uridine kinase (uridine monophosphokinase) (NCBI ptt file), at 194,157 to 194,774
BT0184
BT0185: BT0185 - conserved hypothetical protein (NCBI ptt file), at 194,876 to 195,175
BT0185
BT0186: BT0186 - thiol:disulfide interchange protein dsbD precursor (NCBI ptt file), at 195,185 to 197,245
BT0186
BT0187: BT0187 - conserved hypothetical protein (NCBI ptt file), at 197,341 to 198,045
BT0187
BT0188: BT0188 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 198,238 to 198,840
BT0188
Position (kb) 195
196
197
198 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 194.189 kb on - strand at 194.418 kb on - strand, within BT0184 at 194.451 kb on - strand, within BT0184 at 194.451 kb on - strand, within BT0184 at 194.524 kb on + strand, within BT0184 at 194.648 kb on + strand at 194.680 kb on - strand, within BT0184 at 194.683 kb on - strand, within BT0184 at 194.686 kb on - strand, within BT0184 at 194.766 kb on + strand at 194.820 kb on + strand at 194.845 kb on + strand at 194.874 kb on + strand at 194.899 kb on + strand at 195.089 kb on + strand, within BT0185 at 195.130 kb on + strand, within BT0185 at 197.256 kb on + strand at 197.307 kb on - strand at 197.310 kb on + strand at 197.310 kb on + strand at 197.351 kb on - strand at 197.513 kb on + strand, within BT0187 at 197.639 kb on - strand, within BT0187 at 197.653 kb on - strand, within BT0187 at 197.667 kb on + strand, within BT0187 at 197.693 kb on - strand, within BT0187 at 197.740 kb on - strand, within BT0187 at 197.744 kb on + strand, within BT0187 at 197.745 kb on - strand, within BT0187 at 197.748 kb on + strand, within BT0187 at 197.807 kb on - strand, within BT0187 at 198.121 kb on + strand at 198.122 kb on - strand at 198.210 kb on + strand at 198.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 194,189 - +0.4 194,418 - BT0184 0.42 -2.9 194,451 - BT0184 0.48 +0.0 194,451 - BT0184 0.48 -1.3 194,524 + BT0184 0.59 +0.0 194,648 + -1.6 194,680 - BT0184 0.85 -2.8 194,683 - BT0184 0.85 -1.6 194,686 - BT0184 0.86 -2.0 194,766 + -2.3 194,820 + +3.3 194,845 + +1.0 194,874 + -1.6 194,899 + -1.0 195,089 + BT0185 0.71 -2.0 195,130 + BT0185 0.85 -1.0 197,256 + -2.1 197,307 - -2.0 197,310 + -0.3 197,310 + -1.0 197,351 - +1.2 197,513 + BT0187 0.24 +4.2 197,639 - BT0187 0.42 +0.0 197,653 - BT0187 0.44 +4.0 197,667 + BT0187 0.46 +0.0 197,693 - BT0187 0.50 +0.6 197,740 - BT0187 0.57 -2.3 197,744 + BT0187 0.57 -2.6 197,745 - BT0187 0.57 +0.0 197,748 + BT0187 0.58 -1.0 197,807 - BT0187 0.66 +0.0 198,121 + -1.0 198,122 - +0.9 198,210 + +0.0 198,242 - +0.0
Or see this region's nucleotide sequence