Experiment: BHIS with Cephalothin 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0076 and BT0077 are separated by 76 nucleotides BT0077 and BT0078 are separated by 258 nucleotides BT0078 and BT0079 are separated by 298 nucleotides BT0079 and BT0080 are separated by 43 nucleotides
BT0076: BT0076 - transposase (NCBI ptt file), at 73,184 to 74,416
BT0076
BT0077: BT0077 - conserved hypothetical protein (NCBI ptt file), at 74,493 to 74,999
BT0077
BT0078: BT0078 - putative DNA repair protein (NCBI ptt file), at 75,258 to 75,710
BT0078
BT0079: BT0079 - putative anti-restriction protein (NCBI ptt file), at 76,009 to 76,542
BT0079
BT0080: BT0080 - hypothetical protein (NCBI ptt file), at 76,586 to 76,798
BT0080
Position (kb) 75
76 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 74.299 kb on - strand at 74.316 kb on - strand at 74.345 kb on - strand at 74.404 kb on - strand at 74.419 kb on - strand at 74.419 kb on - strand at 74.419 kb on - strand at 74.470 kb on - strand at 74.483 kb on + strand at 74.500 kb on + strand at 74.765 kb on + strand, within BT0077 at 74.769 kb on - strand, within BT0077 at 74.794 kb on + strand, within BT0077 at 74.795 kb on - strand, within BT0077 at 74.854 kb on - strand, within BT0077 at 74.930 kb on + strand, within BT0077 at 74.931 kb on - strand, within BT0077 at 74.938 kb on + strand, within BT0077 at 74.981 kb on + strand at 74.981 kb on + strand at 74.988 kb on + strand at 75.041 kb on + strand at 75.065 kb on + strand at 75.071 kb on - strand at 75.118 kb on - strand at 75.186 kb on + strand at 75.314 kb on - strand, within BT0078 at 75.338 kb on - strand, within BT0078 at 75.359 kb on + strand, within BT0078 at 75.359 kb on + strand, within BT0078 at 75.360 kb on - strand, within BT0078 at 75.378 kb on + strand, within BT0078 at 75.412 kb on - strand, within BT0078 at 75.458 kb on + strand, within BT0078 at 75.459 kb on - strand, within BT0078 at 75.488 kb on - strand, within BT0078 at 75.521 kb on - strand, within BT0078 at 75.554 kb on + strand, within BT0078 at 75.606 kb on - strand, within BT0078 at 75.640 kb on + strand, within BT0078 at 75.641 kb on - strand, within BT0078 at 75.739 kb on + strand at 75.767 kb on - strand at 75.774 kb on + strand at 75.774 kb on + strand at 75.823 kb on + strand at 75.824 kb on - strand at 75.826 kb on - strand at 75.839 kb on - strand at 75.874 kb on - strand at 75.894 kb on - strand at 75.986 kb on + strand at 76.020 kb on - strand at 76.042 kb on + strand at 76.043 kb on - strand at 76.043 kb on - strand at 76.054 kb on + strand at 76.064 kb on - strand, within BT0079 at 76.064 kb on - strand, within BT0079 at 76.095 kb on - strand, within BT0079 at 76.095 kb on - strand, within BT0079 at 76.329 kb on - strand, within BT0079 at 76.329 kb on - strand, within BT0079 at 76.329 kb on - strand, within BT0079 at 76.354 kb on - strand, within BT0079 at 76.388 kb on - strand, within BT0079 at 76.412 kb on - strand, within BT0079 at 76.459 kb on + strand, within BT0079 at 76.495 kb on - strand at 76.504 kb on + strand at 76.544 kb on + strand at 76.601 kb on - strand at 76.601 kb on - strand at 76.647 kb on - strand, within BT0080 at 76.687 kb on - strand, within BT0080 at 76.691 kb on - strand, within BT0080
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Cephalothin 0.2 mM remove 74,299 - +1.7 74,316 - -3.5 74,345 - -3.0 74,404 - -2.5 74,419 - +0.0 74,419 - -2.2 74,419 - -2.4 74,470 - +1.5 74,483 + +0.0 74,500 + +0.6 74,765 + BT0077 0.54 -1.1 74,769 - BT0077 0.54 -2.3 74,794 + BT0077 0.59 -2.3 74,795 - BT0077 0.60 -1.5 74,854 - BT0077 0.71 -1.0 74,930 + BT0077 0.86 -2.0 74,931 - BT0077 0.86 -2.3 74,938 + BT0077 0.88 -2.0 74,981 + -1.8 74,981 + -2.4 74,988 + +0.0 75,041 + -1.0 75,065 + +2.4 75,071 - +0.0 75,118 - -1.0 75,186 + +2.6 75,314 - BT0078 0.12 -2.4 75,338 - BT0078 0.18 +1.0 75,359 + BT0078 0.22 -2.5 75,359 + BT0078 0.22 +0.8 75,360 - BT0078 0.23 -1.5 75,378 + BT0078 0.26 +0.5 75,412 - BT0078 0.34 -1.8 75,458 + BT0078 0.44 +3.7 75,459 - BT0078 0.44 -2.9 75,488 - BT0078 0.51 +0.0 75,521 - BT0078 0.58 -0.5 75,554 + BT0078 0.65 -2.0 75,606 - BT0078 0.77 -1.4 75,640 + BT0078 0.84 -2.5 75,641 - BT0078 0.85 -1.2 75,739 + -1.7 75,767 - -1.7 75,774 + +0.0 75,774 + +0.0 75,823 + -1.1 75,824 - +0.8 75,826 - +4.2 75,839 - -1.5 75,874 - -2.3 75,894 - -1.5 75,986 + -0.5 76,020 - +0.0 76,042 + -1.8 76,043 - -2.3 76,043 - +0.3 76,054 + -1.0 76,064 - BT0079 0.10 -1.5 76,064 - BT0079 0.10 -2.3 76,095 - BT0079 0.16 +1.6 76,095 - BT0079 0.16 +2.7 76,329 - BT0079 0.60 -1.5 76,329 - BT0079 0.60 -1.5 76,329 - BT0079 0.60 -2.1 76,354 - BT0079 0.65 -1.5 76,388 - BT0079 0.71 -1.0 76,412 - BT0079 0.75 -1.3 76,459 + BT0079 0.84 -1.1 76,495 - -2.8 76,504 + -2.8 76,544 + +1.0 76,601 - -1.5 76,601 - -2.3 76,647 - BT0080 0.29 -2.5 76,687 - BT0080 0.47 -1.5 76,691 - BT0080 0.49 -1.3
Or see this region's nucleotide sequence