Experiment: fumarate electron acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO2736 and bioD are separated by 82 nucleotides bioD and bioC are separated by 54 nucleotides bioC and bioF are separated by 120 nucleotides
SO2736: SO2736 - conserved hypothetical protein (NCBI ptt file), at 2,851,535 to 2,852,503
SO2736
SO2737: bioD - dethiobiotin synthase (NCBI ptt file), at 2,852,586 to 2,853,281
bioD
SO2738: bioC - biotin synthesis protein BioC (NCBI ptt file), at 2,853,336 to 2,854,163
bioC
SO2739: bioF - 8-amino-7-oxononanoate synthase (NCBI ptt file), at 2,854,284 to 2,855,489
bioF
Position (kb)
2853
2854
2855 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2852.340 kb on - strand, within SO2736 at 2852.346 kb on - strand, within SO2736 at 2852.412 kb on + strand at 2852.412 kb on + strand at 2852.412 kb on + strand at 2852.458 kb on - strand at 2852.505 kb on - strand at 2853.392 kb on - strand at 2853.393 kb on - strand at 2853.845 kb on - strand, within bioC at 2853.885 kb on - strand, within bioC at 2853.980 kb on - strand, within bioC at 2854.231 kb on - strand at 2854.277 kb on - strand at 2854.637 kb on - strand, within bioF at 2854.637 kb on - strand, within bioF at 2854.732 kb on - strand, within bioF at 2854.766 kb on - strand, within bioF at 2854.999 kb on - strand, within bioF at 2855.110 kb on - strand, within bioF at 2855.112 kb on - strand, within bioF
Per-strain Table
Position Strand Gene LocusTag Fraction fumarate electron acceptor remove 2,852,340 - SO2736 0.83 -0.4 2,852,346 - SO2736 0.84 -0.2 2,852,412 + +0.0 2,852,412 + -1.7 2,852,412 + -0.4 2,852,458 - +0.5 2,852,505 - +0.3 2,853,392 - +0.1 2,853,393 - +2.1 2,853,845 - bioC SO2738 0.61 +0.1 2,853,885 - bioC SO2738 0.66 +0.1 2,853,980 - bioC SO2738 0.78 -0.4 2,854,231 - +3.9 2,854,277 - +1.9 2,854,637 - bioF SO2739 0.29 -0.9 2,854,637 - bioF SO2739 0.29 -0.4 2,854,732 - bioF SO2739 0.37 +0.1 2,854,766 - bioF SO2739 0.40 -1.4 2,854,999 - bioF SO2739 0.59 -0.9 2,855,110 - bioF SO2739 0.68 +1.1 2,855,112 - bioF SO2739 0.69 +1.1
Or see this region's nucleotide sequence