Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31990 and RR42_RS31995 are separated by 99 nucleotides RR42_RS31995 and RR42_RS32000 are separated by 58 nucleotides RR42_RS32000 and RR42_RS32005 are separated by 19 nucleotides
RR42_RS31990: RR42_RS31990 - delta 4, 5-alpha steroid dehydrogenase, at 2,554,517 to 2,556,223
_RS31990
RR42_RS31995: RR42_RS31995 - flavin reductase, at 2,556,323 to 2,556,841
_RS31995
RR42_RS32000: RR42_RS32000 - 3-oxosteroid 1-dehydrogenase, at 2,556,900 to 2,558,642
_RS32000
RR42_RS32005: RR42_RS32005 - flavin-dependent monooxygenase, at 2,558,662 to 2,559,855
_RS32005
Position (kb)
2556
2557
2558
2559 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2555.974 kb on - strand, within RR42_RS31990 at 2556.043 kb on - strand, within RR42_RS31990 at 2556.378 kb on + strand, within RR42_RS31995 at 2556.378 kb on + strand, within RR42_RS31995 at 2556.545 kb on - strand, within RR42_RS31995 at 2556.805 kb on + strand at 2556.806 kb on - strand at 2556.829 kb on + strand at 2556.830 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2557.211 kb on - strand, within RR42_RS32000 at 2557.211 kb on - strand, within RR42_RS32000 at 2557.214 kb on + strand, within RR42_RS32000 at 2557.215 kb on - strand, within RR42_RS32000 at 2557.502 kb on - strand, within RR42_RS32000 at 2557.526 kb on - strand, within RR42_RS32000 at 2557.766 kb on - strand, within RR42_RS32000 at 2557.998 kb on - strand, within RR42_RS32000 at 2557.998 kb on - strand, within RR42_RS32000 at 2558.291 kb on - strand, within RR42_RS32000 at 2558.318 kb on - strand, within RR42_RS32000 at 2558.348 kb on - strand, within RR42_RS32000 at 2558.416 kb on + strand, within RR42_RS32000 at 2558.417 kb on - strand, within RR42_RS32000 at 2558.668 kb on - strand at 2558.741 kb on + strand at 2558.799 kb on - strand, within RR42_RS32005 at 2558.799 kb on - strand, within RR42_RS32005 at 2559.068 kb on + strand, within RR42_RS32005 at 2559.068 kb on + strand, within RR42_RS32005 at 2559.158 kb on + strand, within RR42_RS32005 at 2559.318 kb on - strand, within RR42_RS32005 at 2559.347 kb on + strand, within RR42_RS32005 at 2559.489 kb on + strand, within RR42_RS32005 at 2559.491 kb on + strand, within RR42_RS32005 at 2559.492 kb on - strand, within RR42_RS32005 at 2559.510 kb on + strand, within RR42_RS32005 at 2559.593 kb on + strand, within RR42_RS32005 at 2559.594 kb on - strand, within RR42_RS32005
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 2,555,974 - RR42_RS31990 0.85 -1.9 2,556,043 - RR42_RS31990 0.89 -1.3 2,556,378 + RR42_RS31995 0.11 -0.2 2,556,378 + RR42_RS31995 0.11 -3.2 2,556,545 - RR42_RS31995 0.43 -1.3 2,556,805 + -0.1 2,556,806 - -0.1 2,556,829 + -0.0 2,556,830 - +0.8 2,556,858 - -0.2 2,556,858 - -3.1 2,556,858 - -0.8 2,556,858 - +0.7 2,557,211 - RR42_RS32000 0.18 +1.0 2,557,211 - RR42_RS32000 0.18 +0.5 2,557,214 + RR42_RS32000 0.18 -1.5 2,557,215 - RR42_RS32000 0.18 +0.2 2,557,502 - RR42_RS32000 0.35 +0.7 2,557,526 - RR42_RS32000 0.36 -0.7 2,557,766 - RR42_RS32000 0.50 +1.0 2,557,998 - RR42_RS32000 0.63 +0.2 2,557,998 - RR42_RS32000 0.63 +0.1 2,558,291 - RR42_RS32000 0.80 +1.8 2,558,318 - RR42_RS32000 0.81 -0.7 2,558,348 - RR42_RS32000 0.83 -1.1 2,558,416 + RR42_RS32000 0.87 -1.8 2,558,417 - RR42_RS32000 0.87 -0.2 2,558,668 - +0.4 2,558,741 + -0.4 2,558,799 - RR42_RS32005 0.11 -1.5 2,558,799 - RR42_RS32005 0.11 +1.1 2,559,068 + RR42_RS32005 0.34 -2.5 2,559,068 + RR42_RS32005 0.34 +0.7 2,559,158 + RR42_RS32005 0.42 -0.2 2,559,318 - RR42_RS32005 0.55 -0.8 2,559,347 + RR42_RS32005 0.57 -1.3 2,559,489 + RR42_RS32005 0.69 +1.9 2,559,491 + RR42_RS32005 0.69 -0.5 2,559,492 - RR42_RS32005 0.70 -2.4 2,559,510 + RR42_RS32005 0.71 -0.0 2,559,593 + RR42_RS32005 0.78 -1.2 2,559,594 - RR42_RS32005 0.78 -0.5
Or see this region's nucleotide sequence