Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS31980 and RR42_RS31985 are separated by 11 nucleotides RR42_RS31985 and RR42_RS31990 overlap by 17 nucleotides RR42_RS31990 and RR42_RS31995 are separated by 99 nucleotides RR42_RS31995 and RR42_RS32000 are separated by 58 nucleotides
RR42_RS31980: RR42_RS31980 - 3-ketosteroid-9-alpha-hydroxylase, at 2,552,726 to 2,553,754
_RS31980
RR42_RS31985: RR42_RS31985 - short-chain dehydrogenase, at 2,553,766 to 2,554,533
_RS31985
RR42_RS31990: RR42_RS31990 - delta 4, 5-alpha steroid dehydrogenase, at 2,554,517 to 2,556,223
_RS31990
RR42_RS31995: RR42_RS31995 - flavin reductase, at 2,556,323 to 2,556,841
_RS31995
RR42_RS32000: RR42_RS32000 - 3-oxosteroid 1-dehydrogenase, at 2,556,900 to 2,558,642
_RS32000
Position (kb)
2554
2555
2556
2557 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2554.011 kb on - strand, within RR42_RS31985 at 2554.011 kb on - strand, within RR42_RS31985 at 2554.080 kb on - strand, within RR42_RS31985 at 2554.126 kb on - strand, within RR42_RS31985 at 2554.126 kb on - strand, within RR42_RS31985 at 2554.126 kb on - strand, within RR42_RS31985 at 2554.860 kb on + strand, within RR42_RS31990 at 2554.941 kb on + strand, within RR42_RS31990 at 2554.941 kb on + strand, within RR42_RS31990 at 2554.942 kb on - strand, within RR42_RS31990 at 2554.942 kb on - strand, within RR42_RS31990 at 2554.942 kb on - strand, within RR42_RS31990 at 2554.942 kb on - strand, within RR42_RS31990 at 2555.370 kb on + strand, within RR42_RS31990 at 2555.410 kb on + strand, within RR42_RS31990 at 2555.411 kb on - strand, within RR42_RS31990 at 2555.808 kb on + strand, within RR42_RS31990 at 2555.812 kb on - strand, within RR42_RS31990 at 2555.817 kb on + strand, within RR42_RS31990 at 2555.818 kb on - strand, within RR42_RS31990 at 2555.818 kb on - strand, within RR42_RS31990 at 2555.974 kb on - strand, within RR42_RS31990 at 2556.043 kb on - strand, within RR42_RS31990 at 2556.378 kb on + strand, within RR42_RS31995 at 2556.378 kb on + strand, within RR42_RS31995 at 2556.545 kb on - strand, within RR42_RS31995 at 2556.805 kb on + strand at 2556.806 kb on - strand at 2556.829 kb on + strand at 2556.830 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2556.858 kb on - strand at 2557.211 kb on - strand, within RR42_RS32000 at 2557.211 kb on - strand, within RR42_RS32000 at 2557.214 kb on + strand, within RR42_RS32000 at 2557.215 kb on - strand, within RR42_RS32000
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 2,554,011 - RR42_RS31985 0.32 -0.1 2,554,011 - RR42_RS31985 0.32 +0.5 2,554,080 - RR42_RS31985 0.41 +0.7 2,554,126 - RR42_RS31985 0.47 -0.9 2,554,126 - RR42_RS31985 0.47 +1.2 2,554,126 - RR42_RS31985 0.47 -0.2 2,554,860 + RR42_RS31990 0.20 +0.7 2,554,941 + RR42_RS31990 0.25 +0.1 2,554,941 + RR42_RS31990 0.25 +1.0 2,554,942 - RR42_RS31990 0.25 +0.6 2,554,942 - RR42_RS31990 0.25 +0.0 2,554,942 - RR42_RS31990 0.25 -0.1 2,554,942 - RR42_RS31990 0.25 -0.5 2,555,370 + RR42_RS31990 0.50 +1.3 2,555,410 + RR42_RS31990 0.52 -1.2 2,555,411 - RR42_RS31990 0.52 -0.5 2,555,808 + RR42_RS31990 0.76 +0.7 2,555,812 - RR42_RS31990 0.76 -1.2 2,555,817 + RR42_RS31990 0.76 -1.6 2,555,818 - RR42_RS31990 0.76 +0.8 2,555,818 - RR42_RS31990 0.76 -0.7 2,555,974 - RR42_RS31990 0.85 -1.9 2,556,043 - RR42_RS31990 0.89 -1.3 2,556,378 + RR42_RS31995 0.11 -0.2 2,556,378 + RR42_RS31995 0.11 -3.2 2,556,545 - RR42_RS31995 0.43 -1.3 2,556,805 + -0.1 2,556,806 - -0.1 2,556,829 + -0.0 2,556,830 - +0.8 2,556,858 - -0.2 2,556,858 - -3.1 2,556,858 - -0.8 2,556,858 - +0.7 2,557,211 - RR42_RS32000 0.18 +1.0 2,557,211 - RR42_RS32000 0.18 +0.5 2,557,214 + RR42_RS32000 0.18 -1.5 2,557,215 - RR42_RS32000 0.18 +0.2
Or see this region's nucleotide sequence