Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15475 and RR42_RS15480 overlap by 4 nucleotides RR42_RS15480 and RR42_RS15485 are separated by 385 nucleotides RR42_RS15485 and RR42_RS15490 are separated by 146 nucleotides RR42_RS15490 and RR42_RS15495 are separated by 325 nucleotides
RR42_RS15475: RR42_RS15475 - protein tolQ, at 3,343,232 to 3,343,915
_RS15475
RR42_RS15480: RR42_RS15480 - acyl-CoA thioesterase, at 3,343,912 to 3,344,316
_RS15480
RR42_RS15485: RR42_RS15485 - serine hydroxymethyltransferase, at 3,344,702 to 3,345,949
_RS15485
RR42_RS15490: RR42_RS15490 - NrdR family transcriptional regulator, at 3,346,096 to 3,346,563
_RS15490
RR42_RS15495: RR42_RS15495 - hypothetical protein, at 3,346,889 to 3,347,170
_RS15495
Position (kb)
3344
3345
3346 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3343.786 kb on + strand, within RR42_RS15475 at 3343.795 kb on + strand, within RR42_RS15475 at 3343.795 kb on + strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.796 kb on - strand, within RR42_RS15475 at 3343.797 kb on + strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.798 kb on - strand, within RR42_RS15475 at 3343.974 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.123 kb on + strand, within RR42_RS15480 at 3344.124 kb on - strand, within RR42_RS15480 at 3344.124 kb on - strand, within RR42_RS15480 at 3344.241 kb on - strand, within RR42_RS15480 at 3344.257 kb on + strand, within RR42_RS15480 at 3344.258 kb on - strand, within RR42_RS15480 at 3344.286 kb on - strand at 3344.329 kb on - strand at 3344.371 kb on + strand at 3344.371 kb on + strand at 3344.371 kb on + strand at 3344.372 kb on - strand at 3344.380 kb on + strand at 3344.380 kb on + strand at 3344.381 kb on - strand at 3344.381 kb on - strand at 3344.512 kb on + strand at 3344.608 kb on + strand at 3346.077 kb on + strand at 3346.276 kb on + strand, within RR42_RS15490 at 3346.278 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.465 kb on + strand, within RR42_RS15490 at 3346.466 kb on - strand, within RR42_RS15490 at 3346.466 kb on - strand, within RR42_RS15490 at 3346.487 kb on - strand, within RR42_RS15490 at 3346.604 kb on - strand at 3346.604 kb on - strand at 3346.650 kb on - strand at 3346.799 kb on + strand at 3346.799 kb on + strand at 3346.799 kb on + strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.800 kb on - strand at 3346.857 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 3,343,786 + RR42_RS15475 0.81 +0.0 3,343,795 + RR42_RS15475 0.82 -1.6 3,343,795 + RR42_RS15475 0.82 -0.3 3,343,796 - RR42_RS15475 0.82 +1.9 3,343,796 - RR42_RS15475 0.82 +1.2 3,343,796 - RR42_RS15475 0.82 +0.1 3,343,796 - RR42_RS15475 0.82 +0.3 3,343,796 - RR42_RS15475 0.82 -0.5 3,343,796 - RR42_RS15475 0.82 -0.3 3,343,797 + RR42_RS15475 0.83 +1.7 3,343,798 - RR42_RS15475 0.83 +0.3 3,343,798 - RR42_RS15475 0.83 -1.0 3,343,798 - RR42_RS15475 0.83 -1.1 3,343,798 - RR42_RS15475 0.83 +0.6 3,343,974 + RR42_RS15480 0.15 +0.0 3,344,123 + RR42_RS15480 0.52 -2.6 3,344,123 + RR42_RS15480 0.52 +0.0 3,344,123 + RR42_RS15480 0.52 +1.8 3,344,124 - RR42_RS15480 0.52 +0.8 3,344,124 - RR42_RS15480 0.52 +0.6 3,344,241 - RR42_RS15480 0.81 -0.8 3,344,257 + RR42_RS15480 0.85 -1.1 3,344,258 - RR42_RS15480 0.85 +1.2 3,344,286 - +0.6 3,344,329 - +1.2 3,344,371 + -1.6 3,344,371 + +0.6 3,344,371 + +1.2 3,344,372 - -2.8 3,344,380 + -0.3 3,344,380 + +0.5 3,344,381 - +0.8 3,344,381 - +1.1 3,344,512 + -1.0 3,344,608 + -1.8 3,346,077 + -1.8 3,346,276 + RR42_RS15490 0.38 -1.6 3,346,278 + RR42_RS15490 0.39 +0.6 3,346,465 + RR42_RS15490 0.79 -1.3 3,346,465 + RR42_RS15490 0.79 +0.9 3,346,465 + RR42_RS15490 0.79 +2.2 3,346,465 + RR42_RS15490 0.79 +3.2 3,346,465 + RR42_RS15490 0.79 +0.2 3,346,466 - RR42_RS15490 0.79 -0.8 3,346,466 - RR42_RS15490 0.79 +0.6 3,346,487 - RR42_RS15490 0.84 -2.5 3,346,604 - -1.8 3,346,604 - +1.2 3,346,650 - -0.1 3,346,799 + -1.8 3,346,799 + -0.1 3,346,799 + +1.2 3,346,800 - -0.3 3,346,800 - -4.2 3,346,800 - +0.7 3,346,800 - -0.9 3,346,800 - -1.9 3,346,800 - -1.8 3,346,800 - +0.5 3,346,800 - +2.8 3,346,800 - +0.4 3,346,800 - +0.2 3,346,857 + +0.4
Or see this region's nucleotide sequence