Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS09345

Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS09335 and RR42_RS09340 are separated by 100 nucleotidesRR42_RS09340 and RR42_RS09345 are separated by 141 nucleotidesRR42_RS09345 and RR42_RS09350 are separated by 303 nucleotides RR42_RS09335: RR42_RS09335 - DNA polymerase II, at 1,974,110 to 1,976,485 _RS09335 RR42_RS09340: RR42_RS09340 - lysine transporter LysE, at 1,976,586 to 1,977,218 _RS09340 RR42_RS09345: RR42_RS09345 - limonene-1,2-epoxide hydrolase, at 1,977,360 to 1,977,803 _RS09345 RR42_RS09350: RR42_RS09350 - AraC family transcriptional regulator, at 1,978,107 to 1,978,682 _RS09350 Position (kb) 1977 1978Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1976.602 kb on + strandat 1976.603 kb on - strandat 1976.603 kb on - strandat 1976.777 kb on + strand, within RR42_RS09340at 1976.783 kb on + strand, within RR42_RS09340at 1976.783 kb on + strand, within RR42_RS09340at 1976.784 kb on - strand, within RR42_RS09340at 1976.804 kb on + strand, within RR42_RS09340at 1976.827 kb on + strand, within RR42_RS09340at 1976.828 kb on - strand, within RR42_RS09340at 1976.828 kb on - strand, within RR42_RS09340at 1976.828 kb on - strand, within RR42_RS09340at 1976.859 kb on - strand, within RR42_RS09340at 1976.865 kb on - strand, within RR42_RS09340at 1976.963 kb on - strand, within RR42_RS09340at 1976.963 kb on - strand, within RR42_RS09340at 1976.963 kb on - strand, within RR42_RS09340at 1976.972 kb on - strand, within RR42_RS09340at 1977.008 kb on - strand, within RR42_RS09340at 1977.059 kb on - strand, within RR42_RS09340at 1977.239 kb on - strandat 1977.242 kb on + strandat 1977.242 kb on + strandat 1977.242 kb on + strandat 1977.242 kb on + strandat 1977.293 kb on + strandat 1977.294 kb on - strandat 1977.329 kb on + strandat 1977.344 kb on + strandat 1977.345 kb on - strandat 1977.387 kb on - strandat 1977.418 kb on + strand, within RR42_RS09345at 1977.418 kb on + strand, within RR42_RS09345at 1977.419 kb on - strand, within RR42_RS09345at 1977.419 kb on - strand, within RR42_RS09345at 1977.419 kb on - strand, within RR42_RS09345at 1977.419 kb on - strand, within RR42_RS09345at 1977.419 kb on - strand, within RR42_RS09345at 1977.520 kb on + strand, within RR42_RS09345at 1977.679 kb on + strand, within RR42_RS09345at 1977.680 kb on - strand, within RR42_RS09345at 1977.680 kb on - strand, within RR42_RS09345at 1977.680 kb on - strand, within RR42_RS09345at 1977.684 kb on + strand, within RR42_RS09345at 1977.684 kb on + strand, within RR42_RS09345at 1977.685 kb on - strand, within RR42_RS09345at 1977.685 kb on - strand, within RR42_RS09345at 1977.798 kb on - strandat 1977.798 kb on - strandat 1977.977 kb on - strandat 1977.983 kb on - strandat 1977.992 kb on + strandat 1978.011 kb on + strandat 1978.011 kb on + strandat 1978.012 kb on - strandat 1978.040 kb on + strandat 1978.040 kb on + strandat 1978.040 kb on + strandat 1978.041 kb on - strandat 1978.041 kb on - strandat 1978.046 kb on - strandat 1978.046 kb on - strandat 1978.049 kb on - strandat 1978.095 kb on + strandat 1978.138 kb on + strandat 1978.196 kb on - strand, within RR42_RS09350at 1978.227 kb on + strand, within RR42_RS09350at 1978.252 kb on - strand, within RR42_RS09350at 1978.306 kb on - strand, within RR42_RS09350at 1978.473 kb on + strand, within RR42_RS09350at 1978.473 kb on + strand, within RR42_RS09350at 1978.474 kb on - strand, within RR42_RS09350at 1978.482 kb on + strand, within RR42_RS09350at 1978.482 kb on + strand, within RR42_RS09350at 1978.483 kb on - strand, within RR42_RS09350at 1978.540 kb on + strand, within RR42_RS09350at 1978.637 kb on - strandat 1978.789 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
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1,976,602 + +0.2
1,976,603 - -1.2
1,976,603 - -3.4
1,976,777 + RR42_RS09340 0.30 +1.6
1,976,783 + RR42_RS09340 0.31 -0.3
1,976,783 + RR42_RS09340 0.31 +0.3
1,976,784 - RR42_RS09340 0.31 +1.0
1,976,804 + RR42_RS09340 0.34 -0.8
1,976,827 + RR42_RS09340 0.38 -0.1
1,976,828 - RR42_RS09340 0.38 -0.1
1,976,828 - RR42_RS09340 0.38 +0.4
1,976,828 - RR42_RS09340 0.38 -2.5
1,976,859 - RR42_RS09340 0.43 -1.2
1,976,865 - RR42_RS09340 0.44 +0.2
1,976,963 - RR42_RS09340 0.60 +0.4
1,976,963 - RR42_RS09340 0.60 -0.5
1,976,963 - RR42_RS09340 0.60 +0.2
1,976,972 - RR42_RS09340 0.61 +1.5
1,977,008 - RR42_RS09340 0.67 +0.2
1,977,059 - RR42_RS09340 0.75 -0.4
1,977,239 - -0.6
1,977,242 + -0.8
1,977,242 + +1.3
1,977,242 + -0.5
1,977,242 + -0.3
1,977,293 + +1.4
1,977,294 - +0.2
1,977,329 + -2.5
1,977,344 + -0.4
1,977,345 - +1.4
1,977,387 - -1.1
1,977,418 + RR42_RS09345 0.13 +0.0
1,977,418 + RR42_RS09345 0.13 +2.5
1,977,419 - RR42_RS09345 0.13 -0.9
1,977,419 - RR42_RS09345 0.13 +2.6
1,977,419 - RR42_RS09345 0.13 -0.5
1,977,419 - RR42_RS09345 0.13 +0.7
1,977,419 - RR42_RS09345 0.13 -0.8
1,977,520 + RR42_RS09345 0.36 -0.2
1,977,679 + RR42_RS09345 0.72 -0.1
1,977,680 - RR42_RS09345 0.72 +0.7
1,977,680 - RR42_RS09345 0.72 +0.6
1,977,680 - RR42_RS09345 0.72 -0.9
1,977,684 + RR42_RS09345 0.73 -0.7
1,977,684 + RR42_RS09345 0.73 +1.1
1,977,685 - RR42_RS09345 0.73 +0.9
1,977,685 - RR42_RS09345 0.73 +1.1
1,977,798 - +0.4
1,977,798 - -0.9
1,977,977 - -2.8
1,977,983 - -0.5
1,977,992 + -2.4
1,978,011 + -1.6
1,978,011 + +0.4
1,978,012 - +0.2
1,978,040 + -0.2
1,978,040 + -2.5
1,978,040 + +0.6
1,978,041 - +0.4
1,978,041 - -1.6
1,978,046 - -0.9
1,978,046 - -1.4
1,978,049 - -0.6
1,978,095 + -0.1
1,978,138 + +2.9
1,978,196 - RR42_RS09350 0.15 -1.4
1,978,227 + RR42_RS09350 0.21 +0.9
1,978,252 - RR42_RS09350 0.25 -0.8
1,978,306 - RR42_RS09350 0.35 -2.2
1,978,473 + RR42_RS09350 0.64 -0.6
1,978,473 + RR42_RS09350 0.64 -0.3
1,978,474 - RR42_RS09350 0.64 -0.8
1,978,482 + RR42_RS09350 0.65 +0.9
1,978,482 + RR42_RS09350 0.65 -1.6
1,978,483 - RR42_RS09350 0.65 +0.2
1,978,540 + RR42_RS09350 0.75 +0.3
1,978,637 - +0.1
1,978,789 + -0.6

Or see this region's nucleotide sequence