Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02320 and RR42_RS02325 are separated by 12 nucleotides RR42_RS02325 and RR42_RS02330 are separated by 34 nucleotides
RR42_RS02320: RR42_RS02320 - trehalose phosphatase, at 520,119 to 520,904
_RS02320
RR42_RS02325: RR42_RS02325 - glucoamylase, at 520,917 to 522,755
_RS02325
RR42_RS02330: RR42_RS02330 - trehalose-6-phosphate synthase, at 522,790 to 524,190
_RS02330
Position (kb)
520
521
522
523 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 519.918 kb on - strand at 519.918 kb on - strand at 520.066 kb on + strand at 520.112 kb on + strand at 520.113 kb on - strand at 520.130 kb on - strand at 520.497 kb on + strand, within RR42_RS02320 at 520.646 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.698 kb on - strand, within RR42_RS02320 at 521.169 kb on + strand, within RR42_RS02325 at 521.169 kb on + strand, within RR42_RS02325 at 521.170 kb on - strand, within RR42_RS02325 at 521.235 kb on - strand, within RR42_RS02325 at 521.271 kb on + strand, within RR42_RS02325 at 521.272 kb on - strand, within RR42_RS02325 at 521.406 kb on + strand, within RR42_RS02325 at 521.407 kb on - strand, within RR42_RS02325 at 521.408 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.457 kb on + strand, within RR42_RS02325 at 521.458 kb on - strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.580 kb on + strand, within RR42_RS02325 at 521.581 kb on - strand, within RR42_RS02325 at 521.651 kb on + strand, within RR42_RS02325 at 521.651 kb on + strand, within RR42_RS02325 at 521.671 kb on - strand, within RR42_RS02325 at 521.671 kb on - strand, within RR42_RS02325 at 521.829 kb on + strand, within RR42_RS02325 at 521.889 kb on + strand, within RR42_RS02325 at 522.010 kb on - strand, within RR42_RS02325 at 522.010 kb on - strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.085 kb on - strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.264 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.473 kb on + strand, within RR42_RS02325 at 522.473 kb on + strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.700 kb on + strand at 522.700 kb on + strand at 522.701 kb on - strand at 522.790 kb on - strand at 522.800 kb on + strand at 522.801 kb on - strand at 523.030 kb on + strand, within RR42_RS02330 at 523.034 kb on - strand, within RR42_RS02330 at 523.034 kb on - strand, within RR42_RS02330 at 523.042 kb on + strand, within RR42_RS02330 at 523.075 kb on + strand, within RR42_RS02330 at 523.121 kb on - strand, within RR42_RS02330 at 523.198 kb on + strand, within RR42_RS02330 at 523.269 kb on + strand, within RR42_RS02330 at 523.342 kb on + strand, within RR42_RS02330 at 523.433 kb on - strand, within RR42_RS02330 at 523.540 kb on + strand, within RR42_RS02330 at 523.541 kb on - strand, within RR42_RS02330 at 523.561 kb on + strand, within RR42_RS02330 at 523.562 kb on - strand, within RR42_RS02330 at 523.603 kb on + strand, within RR42_RS02330 at 523.726 kb on + strand, within RR42_RS02330 at 523.727 kb on - strand, within RR42_RS02330
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 519,918 - -0.1 519,918 - -3.0 520,066 + -0.7 520,112 + -1.7 520,113 - -0.0 520,130 - -0.1 520,497 + RR42_RS02320 0.48 -1.1 520,646 + RR42_RS02320 0.67 -1.8 520,697 + RR42_RS02320 0.74 -2.6 520,697 + RR42_RS02320 0.74 -1.0 520,698 - RR42_RS02320 0.74 +0.4 521,169 + RR42_RS02325 0.14 +1.8 521,169 + RR42_RS02325 0.14 -2.6 521,170 - RR42_RS02325 0.14 +1.2 521,235 - RR42_RS02325 0.17 -0.2 521,271 + RR42_RS02325 0.19 -0.1 521,272 - RR42_RS02325 0.19 +0.1 521,406 + RR42_RS02325 0.27 -0.1 521,407 - RR42_RS02325 0.27 +0.6 521,408 + RR42_RS02325 0.27 -0.3 521,457 + RR42_RS02325 0.29 +1.3 521,457 + RR42_RS02325 0.29 +1.9 521,457 + RR42_RS02325 0.29 -0.3 521,457 + RR42_RS02325 0.29 +0.7 521,458 - RR42_RS02325 0.29 -1.9 521,580 + RR42_RS02325 0.36 -0.1 521,580 + RR42_RS02325 0.36 -0.7 521,580 + RR42_RS02325 0.36 +1.1 521,581 - RR42_RS02325 0.36 +0.2 521,651 + RR42_RS02325 0.40 +0.8 521,651 + RR42_RS02325 0.40 -0.5 521,671 - RR42_RS02325 0.41 -0.5 521,671 - RR42_RS02325 0.41 -1.0 521,829 + RR42_RS02325 0.50 -2.1 521,889 + RR42_RS02325 0.53 -0.7 522,010 - RR42_RS02325 0.59 -1.8 522,010 - RR42_RS02325 0.59 -0.5 522,019 + RR42_RS02325 0.60 +0.3 522,019 + RR42_RS02325 0.60 -1.1 522,019 + RR42_RS02325 0.60 -0.8 522,019 + RR42_RS02325 0.60 +1.1 522,085 - RR42_RS02325 0.64 -0.8 522,179 + RR42_RS02325 0.69 -0.6 522,179 + RR42_RS02325 0.69 +1.5 522,179 + RR42_RS02325 0.69 -0.0 522,264 + RR42_RS02325 0.73 +0.5 522,471 + RR42_RS02325 0.85 -0.9 522,471 + RR42_RS02325 0.85 -1.0 522,471 + RR42_RS02325 0.85 +0.9 522,472 - RR42_RS02325 0.85 -0.1 522,472 - RR42_RS02325 0.85 -1.0 522,472 - RR42_RS02325 0.85 -2.4 522,472 - RR42_RS02325 0.85 -3.1 522,473 + RR42_RS02325 0.85 -0.1 522,473 + RR42_RS02325 0.85 -0.2 522,474 - RR42_RS02325 0.85 +1.0 522,474 - RR42_RS02325 0.85 +1.3 522,474 - RR42_RS02325 0.85 -1.1 522,700 + -1.0 522,700 + -1.0 522,701 - +0.7 522,790 - +0.1 522,800 + +0.4 522,801 - +1.3 523,030 + RR42_RS02330 0.17 +0.9 523,034 - RR42_RS02330 0.17 +0.3 523,034 - RR42_RS02330 0.17 -0.7 523,042 + RR42_RS02330 0.18 +0.9 523,075 + RR42_RS02330 0.20 -1.2 523,121 - RR42_RS02330 0.24 +0.4 523,198 + RR42_RS02330 0.29 -0.7 523,269 + RR42_RS02330 0.34 -0.8 523,342 + RR42_RS02330 0.39 +0.1 523,433 - RR42_RS02330 0.46 -0.2 523,540 + RR42_RS02330 0.54 +0.5 523,541 - RR42_RS02330 0.54 -2.2 523,561 + RR42_RS02330 0.55 +0.0 523,562 - RR42_RS02330 0.55 -0.8 523,603 + RR42_RS02330 0.58 -0.2 523,726 + RR42_RS02330 0.67 -2.4 523,727 - RR42_RS02330 0.67 +1.5
Or see this region's nucleotide sequence