Experiment: Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02305 and RR42_RS02310 are separated by 78 nucleotides RR42_RS02310 and RR42_RS02315 are separated by 15 nucleotides RR42_RS02315 and RR42_RS02320 are separated by 243 nucleotides
RR42_RS02305: RR42_RS02305 - porin, at 516,087 to 517,421
_RS02305
RR42_RS02310: RR42_RS02310 - NADPH-dependent 7-cyano-7-deazaguanine reductase, at 517,500 to 518,333
_RS02310
RR42_RS02315: RR42_RS02315 - threonine dehydratase, at 518,349 to 519,875
_RS02315
RR42_RS02320: RR42_RS02320 - trehalose phosphatase, at 520,119 to 520,904
_RS02320
Position (kb) 518
519
520 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 517.445 kb on + strand at 517.587 kb on - strand, within RR42_RS02310 at 517.700 kb on - strand, within RR42_RS02310 at 517.705 kb on - strand, within RR42_RS02310 at 517.870 kb on + strand, within RR42_RS02310 at 517.870 kb on + strand, within RR42_RS02310 at 517.871 kb on - strand, within RR42_RS02310 at 518.369 kb on - strand at 518.369 kb on - strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.397 kb on + strand at 518.398 kb on - strand at 518.398 kb on - strand at 518.402 kb on - strand at 518.402 kb on - strand at 518.516 kb on + strand, within RR42_RS02315 at 518.587 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.632 kb on + strand, within RR42_RS02315 at 518.757 kb on - strand, within RR42_RS02315 at 518.785 kb on + strand, within RR42_RS02315 at 518.995 kb on + strand, within RR42_RS02315 at 519.264 kb on + strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.265 kb on - strand, within RR42_RS02315 at 519.296 kb on - strand, within RR42_RS02315 at 519.478 kb on + strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.479 kb on - strand, within RR42_RS02315 at 519.491 kb on - strand, within RR42_RS02315 at 519.703 kb on + strand, within RR42_RS02315 at 519.797 kb on - strand at 519.797 kb on - strand at 519.826 kb on + strand at 519.826 kb on + strand at 519.856 kb on + strand at 519.856 kb on + strand at 519.857 kb on - strand at 519.918 kb on - strand at 519.918 kb on - strand at 520.066 kb on + strand at 520.112 kb on + strand at 520.113 kb on - strand at 520.130 kb on - strand at 520.497 kb on + strand, within RR42_RS02320 at 520.646 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.697 kb on + strand, within RR42_RS02320 at 520.698 kb on - strand, within RR42_RS02320
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source 2-Deoxyadenosine 5-monophosphate 5 mM remove 517,445 + +0.9 517,587 - RR42_RS02310 0.10 -1.3 517,700 - RR42_RS02310 0.24 -3.1 517,705 - RR42_RS02310 0.25 -1.0 517,870 + RR42_RS02310 0.44 -2.3 517,870 + RR42_RS02310 0.44 -1.9 517,871 - RR42_RS02310 0.44 -1.5 518,369 - -2.4 518,369 - +0.9 518,397 + -0.2 518,397 + +1.3 518,397 + +0.4 518,398 - -0.3 518,398 - +1.6 518,402 - -0.5 518,402 - -1.3 518,516 + RR42_RS02315 0.11 -2.3 518,587 + RR42_RS02315 0.16 +1.1 518,632 + RR42_RS02315 0.19 +0.0 518,632 + RR42_RS02315 0.19 -0.3 518,632 + RR42_RS02315 0.19 -0.1 518,757 - RR42_RS02315 0.27 +0.6 518,785 + RR42_RS02315 0.29 -3.0 518,995 + RR42_RS02315 0.42 -0.6 519,264 + RR42_RS02315 0.60 -1.7 519,265 - RR42_RS02315 0.60 -1.1 519,265 - RR42_RS02315 0.60 +0.4 519,296 - RR42_RS02315 0.62 -1.3 519,478 + RR42_RS02315 0.74 -1.7 519,479 - RR42_RS02315 0.74 +1.6 519,479 - RR42_RS02315 0.74 +0.5 519,479 - RR42_RS02315 0.74 -2.1 519,479 - RR42_RS02315 0.74 -0.5 519,491 - RR42_RS02315 0.75 +0.5 519,703 + RR42_RS02315 0.89 -0.2 519,797 - -0.9 519,797 - -1.6 519,826 + -3.4 519,826 + -2.0 519,856 + +0.4 519,856 + +0.5 519,857 - -0.2 519,918 - -0.1 519,918 - -3.0 520,066 + -0.7 520,112 + -1.7 520,113 - -0.0 520,130 - -0.1 520,497 + RR42_RS02320 0.48 -1.1 520,646 + RR42_RS02320 0.67 -1.8 520,697 + RR42_RS02320 0.74 -2.6 520,697 + RR42_RS02320 0.74 -1.0 520,698 - RR42_RS02320 0.74 +0.4
Or see this region's nucleotide sequence